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Elisabeth Carolle Ngo Bell, Marie-Anne Vandenhende, Sabrina Caldato, Aurélie Saunier, Pantxika Bellecave, Camille Tumiotto, Véronique Avettand-Fenoel, Mojgan Hessamfar, Philippe Morlat, Fabrice Bonnet, High decay of blood HIV reservoir when tenofovir/emtricitabine/elvitegravir/cobicistat is initiated during the acute primary HIV infection, Journal of Antimicrobial Chemotherapy, Volume 72, Issue 9, September 2017, Pages 2681–2683, https://doi.org/10.1093/jac/dkx166
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Sir,
Early treatment during primary HIV infection (PHI) brings benefits by reducing the circulating viral load and the size of the reservoirs.1 The French guidelines2 propose to treat PHI with a triple antiretroviral combination including two NRTIs plus a third agent: an integrase strand transfer inhibitor (INSTI) or a PI. Among INSTIs, dolutegravir was chosen because of its bioavailability, virological power, genetic barrier and limited drug interactions although there is no clinical trial evaluating it in this context.
The use of the combined form of abacavir/lamivudine/dolutegravir to treat PHI is limited due to the need for human leucocyte antigen (HLA) typing to exclude the abacavir sensitivity conferred by HLA B*57:01. Data on the use of tenofovir/emtricitabine/elvitegravir/cobicistat in this indication are also limited. This elvitegravir combination presents the advantage of a single tablet without risk of hypersensitivity and the cobicistat pharmacokinetic boost allows rapid acquisition of elvitegravir plasma levels compatible with optimal virological efficacy.
We report a case series of patients with PHI treated with tenofovir/emtricitabine/elvitegravir/cobicistat; we monitored blood total HIV DNA load, a biomarker estimating the viral reservoir.3
Six patients were included from April 2014 to August 2015. The plasma HIV RNA load was initially measured by a real-time HIV-1 RT-PCR method (mp2000 Abbott®) with a detection limit of 40 HIV RNA copies/mL and then by a different method (Cobas 6800 Roche®) with a detection limit of 20 HIV RNA copies/mL. The level of total HIV DNA was quantified in whole blood cells by ultrasensitive real-time PCR targeting the long terminal repeat region using the ANRS consensus technique (Biocentric®, France).4 Results are expressed as number of HIV-1 DNA copies/106 PBMCs, with a detection threshold of 8 copies/106 PBMCs. Follow-up was performed up to 18 months.
The six patients were MSM, with a median age of 41 years (range = 27–52). Four of them were Fiebig stage 25 and the two other patients were Fiebig stage 3 and 4, respectively. At initiation of ART, the median HIV RNA was 6.95 log10 (IQR = 6.18 log10–7 log10) with a median CD4 count of 355 cells/mm3 (IQR = 278–500). The median level of HIV DNA was 3.84 log10 copies/106 PBMCs (IQR = 2.73–3.97). The ART was started a median of 4.5 days (IQR = 4–16.5) after the diagnosis of the PHI. The status of optimal viro-immunological responder (OVIR) was defined as HIV RNA <50 copies/mL and HIV DNA <2.3 log10 copies/106 PBMCs, as well as a normalization of immune reconstitution markers: absolute CD4+ T lymphocyte count >500 cells/mm3, percentage of CD4 T cells >30% and CD4+/CD8+ ratio >1.6 Five patients obtained OVIR status after a median of 84 days (IQR = 28–336) of treatment. All patients had undetectable HIV RNA at a median of 75 days (IQR = 30–120). The median gain of CD4 at the 12th month was 432 cells/mm3 (IQR = 203–640) and the median decrease in HIV RNA at the 1st month was 5.03 log10 (IQR = 4.58 log10–5.3 log10). Two patients reached undetectable blood HIV DNA before the 6th month, but had subsequently low levels of HIV DNA (1.43 and 1.23 log10 copies/106 PBMCs for one patient and 1.86 log10 copies/106 PBMCs for the other patient). The clinical and viro-immunological characteristics of all six patients are summarized in Table 1.
Patient . | Age (years) . | Sex . | Delay of treatment (days) . | Origin . | Fiebig stage . | Length of treatment (months) . | HIV RNA copies/mL . | HIV DNA copies/106 PBMCs . | CD4 cells/mm3 . |
---|---|---|---|---|---|---|---|---|---|
1 | 47 | male | 4 | France | 2 | D0 | 1 543 000 | 270 | 500 |
M1 | 46 | 59 | 1144 | ||||||
M2 | <40 | 37 | 1129 | ||||||
M4 | <40 | <8 | 1012 | ||||||
M6 | <40 | 27 | NA | ||||||
M12 | <20 | 17 | 1317 | ||||||
M18 | <20 | <8 | 1183 | ||||||
2 | 39 | male | NA | France | 2 | D0 | 10 000 000 | NA | 177 |
M2 | 74 | 264 | 395 | ||||||
M4 | <20 | 107 | NA | ||||||
M8 | 24 | 90 | NA | ||||||
M12 | 36 | 155 | 380 | ||||||
M18 | <20 | 8 | 561 | ||||||
3 | 43 | male | 28 | France | 4 | D0 | 86 053 | NA | 532 |
M1 | <40 | 129 | 662 | ||||||
M4 | <20 | 109 | 1484 | ||||||
M8 | <20 | <70 | NA | ||||||
M12 | 69 | 182 | 991 | ||||||
M18 | <20 | 63 | 1104 | ||||||
4 | 34 | male | 4 | France | 2 | D0 | 10 000 000 | NA | 278 |
M1 | 51 | 9 | NA | ||||||
M3 | 43 | 43 | NA | ||||||
M7 | <20 | 191 | 762 | ||||||
M12 | <20 | 210 | 683 | ||||||
M18 | <20 | <8 | 635 | ||||||
5 | 27 | male | NA | France | 2 | D0 | 8 469 000 | 9386 | 297 |
M1 | 221 | 373 | 658 | ||||||
M3 | 0 | 82 | 851 | ||||||
M7 | 0 | 286 | 799 | ||||||
M12 | 29 | 34 | 937 | ||||||
M18 | 34 | 137 | 619 | ||||||
6 | 52 | male | 5 | France | 3 | D0 | 9 740 000 | 6947 | 413 |
M1 | <20 | <8 | 586 | ||||||
M3 | <20 | 74 | 618 | ||||||
M6 | <20 | NA | 517 | ||||||
M12 | <20 | <8 | 526 |
Patient . | Age (years) . | Sex . | Delay of treatment (days) . | Origin . | Fiebig stage . | Length of treatment (months) . | HIV RNA copies/mL . | HIV DNA copies/106 PBMCs . | CD4 cells/mm3 . |
---|---|---|---|---|---|---|---|---|---|
1 | 47 | male | 4 | France | 2 | D0 | 1 543 000 | 270 | 500 |
M1 | 46 | 59 | 1144 | ||||||
M2 | <40 | 37 | 1129 | ||||||
M4 | <40 | <8 | 1012 | ||||||
M6 | <40 | 27 | NA | ||||||
M12 | <20 | 17 | 1317 | ||||||
M18 | <20 | <8 | 1183 | ||||||
2 | 39 | male | NA | France | 2 | D0 | 10 000 000 | NA | 177 |
M2 | 74 | 264 | 395 | ||||||
M4 | <20 | 107 | NA | ||||||
M8 | 24 | 90 | NA | ||||||
M12 | 36 | 155 | 380 | ||||||
M18 | <20 | 8 | 561 | ||||||
3 | 43 | male | 28 | France | 4 | D0 | 86 053 | NA | 532 |
M1 | <40 | 129 | 662 | ||||||
M4 | <20 | 109 | 1484 | ||||||
M8 | <20 | <70 | NA | ||||||
M12 | 69 | 182 | 991 | ||||||
M18 | <20 | 63 | 1104 | ||||||
4 | 34 | male | 4 | France | 2 | D0 | 10 000 000 | NA | 278 |
M1 | 51 | 9 | NA | ||||||
M3 | 43 | 43 | NA | ||||||
M7 | <20 | 191 | 762 | ||||||
M12 | <20 | 210 | 683 | ||||||
M18 | <20 | <8 | 635 | ||||||
5 | 27 | male | NA | France | 2 | D0 | 8 469 000 | 9386 | 297 |
M1 | 221 | 373 | 658 | ||||||
M3 | 0 | 82 | 851 | ||||||
M7 | 0 | 286 | 799 | ||||||
M12 | 29 | 34 | 937 | ||||||
M18 | 34 | 137 | 619 | ||||||
6 | 52 | male | 5 | France | 3 | D0 | 9 740 000 | 6947 | 413 |
M1 | <20 | <8 | 586 | ||||||
M3 | <20 | 74 | 618 | ||||||
M6 | <20 | NA | 517 | ||||||
M12 | <20 | <8 | 526 |
D0, day 0, M, month; NA, not available.
Patient . | Age (years) . | Sex . | Delay of treatment (days) . | Origin . | Fiebig stage . | Length of treatment (months) . | HIV RNA copies/mL . | HIV DNA copies/106 PBMCs . | CD4 cells/mm3 . |
---|---|---|---|---|---|---|---|---|---|
1 | 47 | male | 4 | France | 2 | D0 | 1 543 000 | 270 | 500 |
M1 | 46 | 59 | 1144 | ||||||
M2 | <40 | 37 | 1129 | ||||||
M4 | <40 | <8 | 1012 | ||||||
M6 | <40 | 27 | NA | ||||||
M12 | <20 | 17 | 1317 | ||||||
M18 | <20 | <8 | 1183 | ||||||
2 | 39 | male | NA | France | 2 | D0 | 10 000 000 | NA | 177 |
M2 | 74 | 264 | 395 | ||||||
M4 | <20 | 107 | NA | ||||||
M8 | 24 | 90 | NA | ||||||
M12 | 36 | 155 | 380 | ||||||
M18 | <20 | 8 | 561 | ||||||
3 | 43 | male | 28 | France | 4 | D0 | 86 053 | NA | 532 |
M1 | <40 | 129 | 662 | ||||||
M4 | <20 | 109 | 1484 | ||||||
M8 | <20 | <70 | NA | ||||||
M12 | 69 | 182 | 991 | ||||||
M18 | <20 | 63 | 1104 | ||||||
4 | 34 | male | 4 | France | 2 | D0 | 10 000 000 | NA | 278 |
M1 | 51 | 9 | NA | ||||||
M3 | 43 | 43 | NA | ||||||
M7 | <20 | 191 | 762 | ||||||
M12 | <20 | 210 | 683 | ||||||
M18 | <20 | <8 | 635 | ||||||
5 | 27 | male | NA | France | 2 | D0 | 8 469 000 | 9386 | 297 |
M1 | 221 | 373 | 658 | ||||||
M3 | 0 | 82 | 851 | ||||||
M7 | 0 | 286 | 799 | ||||||
M12 | 29 | 34 | 937 | ||||||
M18 | 34 | 137 | 619 | ||||||
6 | 52 | male | 5 | France | 3 | D0 | 9 740 000 | 6947 | 413 |
M1 | <20 | <8 | 586 | ||||||
M3 | <20 | 74 | 618 | ||||||
M6 | <20 | NA | 517 | ||||||
M12 | <20 | <8 | 526 |
Patient . | Age (years) . | Sex . | Delay of treatment (days) . | Origin . | Fiebig stage . | Length of treatment (months) . | HIV RNA copies/mL . | HIV DNA copies/106 PBMCs . | CD4 cells/mm3 . |
---|---|---|---|---|---|---|---|---|---|
1 | 47 | male | 4 | France | 2 | D0 | 1 543 000 | 270 | 500 |
M1 | 46 | 59 | 1144 | ||||||
M2 | <40 | 37 | 1129 | ||||||
M4 | <40 | <8 | 1012 | ||||||
M6 | <40 | 27 | NA | ||||||
M12 | <20 | 17 | 1317 | ||||||
M18 | <20 | <8 | 1183 | ||||||
2 | 39 | male | NA | France | 2 | D0 | 10 000 000 | NA | 177 |
M2 | 74 | 264 | 395 | ||||||
M4 | <20 | 107 | NA | ||||||
M8 | 24 | 90 | NA | ||||||
M12 | 36 | 155 | 380 | ||||||
M18 | <20 | 8 | 561 | ||||||
3 | 43 | male | 28 | France | 4 | D0 | 86 053 | NA | 532 |
M1 | <40 | 129 | 662 | ||||||
M4 | <20 | 109 | 1484 | ||||||
M8 | <20 | <70 | NA | ||||||
M12 | 69 | 182 | 991 | ||||||
M18 | <20 | 63 | 1104 | ||||||
4 | 34 | male | 4 | France | 2 | D0 | 10 000 000 | NA | 278 |
M1 | 51 | 9 | NA | ||||||
M3 | 43 | 43 | NA | ||||||
M7 | <20 | 191 | 762 | ||||||
M12 | <20 | 210 | 683 | ||||||
M18 | <20 | <8 | 635 | ||||||
5 | 27 | male | NA | France | 2 | D0 | 8 469 000 | 9386 | 297 |
M1 | 221 | 373 | 658 | ||||||
M3 | 0 | 82 | 851 | ||||||
M7 | 0 | 286 | 799 | ||||||
M12 | 29 | 34 | 937 | ||||||
M18 | 34 | 137 | 619 | ||||||
6 | 52 | male | 5 | France | 3 | D0 | 9 740 000 | 6947 | 413 |
M1 | <20 | <8 | 586 | ||||||
M3 | <20 | 74 | 618 | ||||||
M6 | <20 | NA | 517 | ||||||
M12 | <20 | <8 | 526 |
D0, day 0, M, month; NA, not available.
The impact of early ART in PHI is now well validated given the clinical, virological, immunological and epidemiological benefits.7,8 Only one study reported the use of tenofovir/emtricitabine/elvitegravir/cobicistat in PHI, with a gain of 350 CD4 cells at week 48 and a rapid decrease in viral load at week 4.9 Our study confirms these results by showing an early and sustained virological response and provides additional data on the decrease in HIV DNA in PHI treated with tenofovir/emtricitabine/elvitegravir/cobicistat.
The OVIR status was obtained in five of six patients less than 120 days after ART initiation. This may reflect the correct diffusion in sanctuaries, although there are no data on the diffusion of INSTIs in the CNS and the digestive compartment.
The natural polymorphism of the integrase gene varies and minor mutations may affect the efficacy of INSTIs.10 Thus, a prevalence around 1.5% of polymorphic INSTI mutations was described in the French PHI cohort (FPC), with a predominance of the E157Q mutation. In addition, transmitted viruses may carry NRTI mutations (prevalence of 5.2% in the FPC), affecting the tenofovir/emtricitabine backbone and then weakening elvitegravir, a molecule presenting a lower genetic barrier to resistance than dolutegravir.11
Tenofovir/emtricitabine/elvitegravir/cobicistat use requires vigilance when prescribed during PHI and there is a need to check the results of drug resistance testing as soon as possible after ART initiation to exclude the presence of any transmitted or polymorphic drug resistance mutation.12
Tenofovir/emtricitabine/elvitegravir/cobicistat is a potent ART, which can reduce the HIV reservoir when initiated swiftly following the PHI. Further data are needed to confirm full diffusion of the drugs in the reservoirs and to compare with other antiretroviral combinations. This combination could represent a therapeutic option in the management of PHI.
Funding
This study was carried out as part of our routine work.
Transparency declarations
V. A.-F. declares to have received reimbursement for attending a symposium from Janssen. P. M. declares to have received reimbursement for attending a symposium from ViiV Healthcare, Gilead, Bristol-Myers Squib, Merck and Janssen. F. B. declares to have received reimbursement for attending a symposium from ViiV Healthcare, Gilead, Bristol-Myers Squib, Merck and Janssen, a fee for speaking and consultancy fee from ViiV Healthcare, Gilead, Bristol-Myers Squib, Merck and Janssen, and funds for research from Gilead and ViiV Healthcare. All other authors: none to declare.