Collaborative Study of the Determination of Menadione Sodium Bisulfite in Injections

Abstract

A chromatographic-UV spectrophotometric method for the determination of menadione sodium bisulfite was subjected to a collaborative study. A sample of the injection is mixed with Celite and placed on a chromatographic column. The column is washed with chloroform; the menadione is eluted with an ammonia-saturated chloroform solution and determined by measuring UV absorbance at 334 nm. Four synthetic sample solutions were sent to 13 collaborators for analysis. The average recoveries of these solutions ranged from 97.5 to 98.4% of added amount and had coefficients of variation ranging from 1.1 to 1.4%. The method has been adopted as official first action.