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Takashi Suzuki, Masahiro Nakagawa, Ayumu Yoshikawa, Noboru Sasagawa, Tamotsu Yoshimori, Yoshinori Ohsumi, Ichizo Nishino, Shoichi Ishiura, Ikuya Nonaka, The First Molecular Evidence That Autophagy Relates Rimmed Vacuole Formation in Chloroquine Myopathy, The Journal of Biochemistry, Volume 131, Issue 5, May 2002, Pages 647–651, https://doi.org/10.1093/oxfordjournals.jbchem.a003147
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Abstract
Chloroquine myopathy is a drug poisoning disease involving rimmed vacuole formation. By Western blot analysis, we investigated posttranslational modification of LC3 in cultured cells with a high concentration of chloroquine, and found that the autophagosome membrane-bound form of LC3 increased dose-dependently. We also constructed a disease model by excessive chloroquine injection into rats and unusual immunohistochem-ical alteration was chased using anti-LC3 antibodies. With chloroquine treatment, muscle atrophy occurred predominantly in soleus muscle and unusual autophagosomes were accumulated. Therefore, we concluded that autophagy plays an important role in rimmed vacuole formation in certain muscular atrophies.