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Koichiro KISHP, Haruhiko NODA, Laser Raman Studies on Myosin, C-Protein, and Myosin-C-Protein Complex, The Journal of Biochemistry, Volume 94, Issue 2, July 1983, Pages 353–359, https://doi.org/10.1093/oxfordjournals.jbchem.a134363
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Abstract
The Raman spectra of purified myosin, C-protein and myosin-C-protein complex in aqueous solution, as well as that of pelleted filaments, were analyzed. The analyses confirmed that C-protein adopts a predominantly non-α-helical structure, while myosin adopts a predominantly :alpha;-helical structure. Neither 10 mm MgCl2 nor filament formation induced large conformational changes of purified myosin. Thermal denaturation of purified myosin, however, induced large changes in the Raman spectrum owing to conformational changes. It is likely that the binding of myosin and C-protein is not accompanied by large conformational changes of the proteins.