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Brandon Brei, John D. Edman, Ben Gerade, J. Marshall Clark, Relative Abundance of Two Cuticular Hydrocarbons Indicates Whether a Mosquito Is Old Enough to Transmit Malaria Parasites, Journal of Medical Entomology, Volume 41, Issue 4, 1 July 2004, Pages 807–809, https://doi.org/10.1603/0022-2585-41.4.807
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Abstract
Temporal changes in the cuticular hydrocarbons of female Anopheles stephensi (Liston) (Diptera: Culicidae) were quantified using gas-liquid chromatography with flame-ionization detection. The ratio of two prominent hydrocarbons, nonacosane (C29) and hentriacontane (C31), was found to change significantly with respect to mosquito age over a period of 15 d. A regression model was developed using this ratio, C29/C31 = 3.96 - 1.63 log (age), and prediction intervals, based on a 12-d developmental interval necessary for females to transmit malaria, were generated using confidence levels for one-sided tests. The model predicted that females that had a C29/C31 ratio of 2.6 or greater were only 10% probable to be old enough to transmit malaria, whereas females with ratios of 1.8 or less were 90% probable.