Increased sensitivity of routine laboratory detection of Strongyloides stercoralis and hookworm by agar-plate culture

Abstract

The efficacy of agar-plate culture has been evaluated for the detection of Strongyloides stercoralis and hookworm, compared with direct smear, the formalin-ether sedimentation technique and the filter-paper method. Of 1085 stool samples from the routine laboratory service at King Chulalongkom Memorial Hospital in Bangkok, 241 samples harboured S. stercoralis, 153 hookworm and 2 Rhabditis hominis. The recovery rate of S. stercoralis by agar-plate culture is significantly superior to the other methods (P < 0·005). The ratios of positive results from the methods used to the total number of S. stercoralis-positive cases were as follows: 1:1·03 by agar-plate culture, 1:1·85 by the filter-paper method, 1:1·98 by the sedimentation technique and 1:10·48 by direct stool smear. A similar trend of the efficacy ratio of each method was obtained for hookworm detection. The characteristic furrows left by hookworm larvae, and larvae and adults of S. stercoralis could be used for preliminary species identification. Daily search for furrows on agar plates for up to 6 consecutive days resulted in an increased sensitivity for diagnosis of both S. stercoralis and hookworm infections.

Strongyloides stercoralis, Necator americanus, hookworm, diagnosis, detection, agar-plate culture