Fig. 5 Localization of OsNHX1–sGFP fusion protein expressed in rice cells. OsNHX1–sGFP fusion protein was expressed in rice cells as described in Materials and Methods. Green fluorescence of sGFP in the cells was imaged under a laser-scanning confocal microscope (A-a and B-a). Tonoplasts are stained red with FM4-64 (B-b). The two images of B-a and B-b are overlaid in B-c. Contrast images of the cells are shown in A-b and B-d. N, nucleus; V, vacuole. Scale bar in A = 50 µm; scale bar in B = 10 µm.