The current study examined the sensitivity of the Comprehensive Trail Making Test (CTMT Reynolds) to neurocognitive deficits in adolescents with traumatic brain injury (TBI). Participants included 60 adolescents, 30 who had sustained TBI and 30 healthy controls (HC) that were individually matched to the TBI sample on age, gender, ethnicity, and geographical region. For both the TBI and HC groups the mean age was 15.0 years (S.D.?2.3 years, range?11–19). The TBI group had a mean IQ of 81.7 (S.D.?14.9), had sustained moderate to severe brain injury, and was assessed an average of 21.1 months (S.D.?20.7) following injury. The TBI group performed approximately 2 standard deviations below the control sample mean on each of the five CTMT trails as well as on the composite index and these differences were significant (p<.001). Significant correlations were present between the CTMT trails and clinical variables associated with brain injury severity. Finally, receiver operating characteristic analyses indicated good classification of the TBI and control cases for the CTMT, although some variability in classification accuracy was present among the various trails. Results suggest that the CTMT is sensitive to TBI in adolescents but continued research is needed with larger samples of individuals with TBI and other types of neurological disorders to further establish the present findings.