Objective: This study looked at the differences in brain function at concentration and baseline between adults who endorsed high anxiety symptomatology and those who endorsed low anxiety symptomatology using single-photon emission computerized tomography (SPECT) scans. Method: Participants were 2990 adults with high anxiety (M age = 37.76, SD = 13.55, male = 47%, Caucasian = 69%) and 2914 adults with low anxiety (M age = 43.37, SD = 18.05, male = 70%, Caucasian = 69%). Subjects were derived from a de-identified database. Subjects were selected in the high and low anxiety groups depending on their endorsement of anxiety symptomatology. The seventeen brain regions analyzed include the limbic area, basal ganglia, cerebellum, frontal areas, occipital areas, parietal areas, temporal areas, motor-sensory areas, and the vermis. Result: A MANOVA found significant differences at baseline for the limbic (right and left) and left basal ganglia regions. Significant differences were found at concentration for the limbic (right and left), basal ganglia (right and left), and vermis regions. All results were significant at p < .01. Conclusion: Results suggest that the functional abilities of these regions are implicated in adults with high anxiety, and this may account for their difficulties in processing of threatening stimuli and regulation of emotions. The limbic system, basal ganglia, and the vermis are related to the processing of emotional information, motivation, and inability to control behavior. This suggests that the emotional center of the brain as well as some of the structures that include a limbic component is implicated in anxiety symptomatology.