Objective: Sleep is known to be critical to a wide range of restorative and recuperative functions. When sleep is significantly disrupted, it may interfere with recovery from Mild Traumatic Brain Injury (MTBI). The purpose of this study is to examine the relation between sleep disruption and health-related quality of life following MTBI. Method: Participants were 139 U.S. service members (89 MTBI, 49 Controls; Age: M = 33.8, SD = 9.0; 89.9% male) enrolled from Walter Reed National Military Medical Center. Participants completed the Traumatic Brain Injury Quality of Life scale (TBI-QOL), Neurobehavioral Symptom Inventory (NSI), and PTSD Checklist (PCLC), on average, 33.3 months post-injury (SD = 38.3). Participants were classified into two ‘Sleep’ categories using select items from the NSI and PCLC: MTBI-Poor Sleep (n = 50), MTBI-Good Sleep (n = 39), Control-Poor Sleep (n = 19), Control-Good Sleep (n = 31). Results: Participants in the MTBI-Poor Sleep group had significantly worse scores on the NSI and PCLC (p < .001, d = 1.65 and d = 1.37 respectively) and all TBI-QOL scales (e.g., fatigue; pain; headaches; depression; anxiety; grief/loss; anger; cognitive complaints; resilience: all p < .002; d = .67–1.16), compared to the MTBI-Good Sleep group. When compared to Controls, the MTBI-Poor Sleep group consistently had the highest number of worse TBI-QOL scales overall (e.g., 6 or more scales = 58%) followed by the Control-Poor Sleep (36.8%), MTBI-Good Sleep (12.8%), and Control-Good Sleep (6.5%) groups. Conclusion: These results suggest that sleep disruption in both MTBI and non-MTBI populations plays a key role in overall self-reported health-related quality of life. However, significantly higher rates of symptoms are found in those persons with increased sleep disruption following MTBI.