Objective: The aim of this study was to analyze the effect of age and education on cognitive performance in healthy individuals from Latin American. Method: The sample consisted of 5,363 healthy adult participants from 12 countries in Latin America. The majority of the participants were women (61.5%), the average age was 53.83 years (range 18–95), and the average education was 9.92 years. Subjects were grouped into three educational levels, 0–5, 6–11, and 12 or more years, and four age ranges, 18–30, 31–50, 51–65 and 66 or more years. All were administered the same set of neuropsychological tests, including assessments of orientation, attention, memory, language, visuoperceptual abilities, motor skills, and executive functions. Results: A between-subjects multivariate analysis of variance (MANOVA) was conducted with age and education as independent variables, and with 10 neuropsychological tests as dependent variables. There were significant differences between age group on the combined dependent variables, F(90, 2089) = 6.40, p = .001; Wilk's Λ = 0.482, partial η2 = .22, and education level, F(60, 1396) = 6.28, p = .001; Wilk's Λ =0.620, partial η2 = .21. When the results were considered separately for age and education level, all 10 neuropsychological tests reach significance. In general, an inspection of the mean scores indicated those younger individuals and those with higher education reporting slightly better performance on all neuropsychological tests. Conclusion: Results suggest that demographic adjustment is beneficial when demographic variables are strongly related to test scores. Thus, these results suggest that adjusting test scores for age and education might have positive effects on validity of test scores.