Objective: White matter integrity (WMI) decreases in older adulthood. Lutein (L) and zeaxanthin (Z) are two carotenoids that diffusely accumulate in brain tissue and positively impact cognition. The current study tested the relation of L + Z to WMI using diffusion tensor imaging (DTI). Method: 38 younger (M = 20.54 years) and 54 older adults (M = 71.87 years) were recruited. Diffusivity was quantified as fractional anisotropy (FA), radial diffusivity (RD), and axial diffusivity (AD). L + Z were measured via retinal (macular pigment optical density – MPOD) and blood serum levels. Hierarchical linear regression was conducted to predict diffusivity in global WM and 4 regions of interest (ROIs – genu of the corpus callosum, cingulum, uncinate fasciculus, and fornix) from L + Z and age group. Results: Age group, but not L + Z, predicted global WMI and diffusivity in the ROIs (R2 = 0.311 – 0.613, p < .001). However, exploratory whole brain analysis in the older adult sample revealed widespread associations between MPOD, serum L + Z, and diffusivity in frontal, temporal, parietal, and occipital white matter, including superior and inferior longitudinal fasciculi, frontal-occipital fasciculus, internal and external capsules, anterior thalamic radiation, body and splenium of the corpus callosum, cerebral peduncles, corticospinal tract, and corona radiata (p < .001, minimal voxel cluster = 8). Conclusion: This study is the first to examine the relation of L + Z to WMI using in vivo methodology. Consistent with previous literature, findings suggest that L + Z are related to WM across diffuse brain regions. The relation of L + Z to WMI may explain one mechanism by which L + Z positively impact cognition.