Abstract

We describe the cytologic features of chondromyxoid fibroma in fine-needle aspiration biopsy specimens in 4 patients. We analyzed rapid Romanowsky– and Papanicolaou-stained slides with a respect to overall cellularity, the presence of hypercellular chondromyxoid fragments (HCFs), nuclear atypia, cytoplasmic features, and the presence of giant cells.

The most consistent and diagnostically useful feature was the presence of HCFs, which were present in all 4 cases. Mild to moderate nuclear atypia was additionally present in all 4 cases. Most cases were hypocellular (3/4). Giant cells were present in 2 cases, with 1 case exhibiting a hypercellular smear with numerous giant cells. All 4 cases were confirmed in subsequent histologic biopsy material. Strict attention to clinical, radiographic, and cytologic features, especially the presence of HCFs, can aid in the specific diagnosis of chondromyxoid fibroma on FNAB.

Chondromyxoid fibroma (CMF) is a benign neoplasm accounting for fewer than 1% of all bone tumors. It has a predilection for men in the second and third decades of life with the metaphyseal regions of the long bones of the lower extremities as the favored sites of occurrence. Other sites include the small bones of the feet and the ilium.1–5 Radiographically, CMF manifests as an eccentrically situated, well-circumscribed, oval metaphyseal lesion parallel to the long axis of the bone. Relatively few cases have been reported in fine-needle aspiration biopsy (FNAB) specimens.6–11 This study presents 4 cases of CMF in FNAB. The cytologic findings and usefulness of FNAB in the diagnosis of CMF are emphasized.

Materials and Methods

Rapid Romanowsky– and Papanicolaou-stained smears from 4 cases of CMF on FNAB specimens were selected from a review of our files. The cases were analyzed with respect to overall cellularity, presence of hypercellular chondromyxoid fragments (HCFs), nuclear atypia, cytoplasmic features, and the presence of giant cells. We define HCFs as small chondromyxoidal mesenchymal masses in which variable numbers of benign-appearing, elongated, and stellate contoured stromal cells are present. In addition, clinical and radiographic features were reviewed.

Results

Table 1 shows the clinical, radiographic, and cytologic features of the 4 cases.

Table 1

Clinicopathologic Features of Four Cases of Chondromyxoid Fibroma

Clinical Features

The patients ranged in age from 15 to 56 years (mean age, 38.5 years) with an equal sex distribution. The involved sites were the metaphysis of the left proximal fibula, left distal humerus, left proximal fibula, and left proximal ischium.

Radiographic Features

In all 4 patients, radiographic studies revealed a well-delineated, expansile, lytic bone lesion that ranged in maximal size from 1.5 to 6.0 cm (mean, 3.7 cm).

Cytologic Features

The majority of cases exhibited low smear cellularity (3/4), mild to moderate degrees of nuclear atypia (4/4), and moderate volumes of foamy cytoplasm (4/4). Diagnostic HCFs were present in all 4 cases. Giant cells were present in 2 of 4 cases) and were numerous in 1 specimen Image 1, Image 2, and Image 3. The most reliable and consistent diagnostic cytomorphologic feature was the presence of HCFs. HCFs consisted of stellate to spindle-shaped cells embedded in a metachromatic chondromyxoid stroma that was brightly stained with rapid Romanowsky. With the Papanicolaou stain, this appeared as a rather pale green substance. No true hyaline cartilaginous fragments with cells in lacunae were observed. All 4 cases were confirmed on subsequent histologic biopsy material that showed a lobulated architecture consisting of an admixture of immature cartilage, fibrous tissue, and stellate cells in a chondromyxoid stroma (Images 1–3). We believe the HCFs are derived from this last tissue component.

Discussion

Although a number of studies of FNAB specimens from malignant bone neoplasms have been published, only a few reports have described in detail the cytologic features of benign chondroid neoplasms. Our study confirms the FNAB findings of CMF previously reported but, in addition, provides a comparison of the cytologic attributes in a series of 4 cases. As only 1 case showed high smear cellularity, most exhibited low cellularity, representing a potential pitfall in underdiagnosis. HCFs were the most reliable cytologic feature present for the diagnosis of CMF in FNAB specimens compared with other studies. Careful attention to the clinical, radiographic, and cytologic features, especially the presence of HCFs, permitted the diagnosis of CMF in all 4 cases, which was subsequently confirmed on histologic material.

The differential diagnosis of CMF in FNAB specimens includes chondroblastoma, giant cell tumor, enchondroma, and low-grade chondrosarcoma. True lacunar spaces with chondrocytes are present in enchondroma and chondrosarcoma; however, they are much less often found in CMF. Chondroblastomas contain a more uniform population of mononuclear cells with round to ovoid nuclei and nuclear grooves compared with a more polymorphous population of stellate, ovoid, and spindle cells (HCFs) found in CMF. Chondromyxoid matrix is also more commonly found in CMF compared with chondroblastomas. Nevertheless, there can be considerable overlapping cytomorphologic features, which may make it difficult to distinguish between these 2 entities. While nuclear pleomorphism can occur in CMF, the pleomorphic cells are more apt to have a more smudged, condensed chromatin lacking the more primitive nuclear chromatin with prominent nucleoli and nuclear irregularity characteristic of high-grade chondrosarcoma. Although giant cells may commonly occur in CMF, they were numerous in only 1 of 4 cases in our series. Furthermore, giant cells are found in both neoplasms and are generally not helpful in distinguishing between them. The epiphyseal location of chondroblastoma is usually not the case for CMF, showing the importance of totally incorporating the clinical and radiologic data into final diagnoses. The epiphyseal location of chondroblastoma is not the case for CMF. The potential pitfall of chondrosarcoma mistaken for CMF on cytologic examination, as well as on histologic examination, has been reported.12,13

Image 1

(Case 1) A, Fine-needle aspiration biopsy (FNAB) specimen in chondromyxoid fibroma (CMF). Hypercellular chondromyxoid fragments containing ovoid cells with smudged nuclear features in a chondromyxoid stoma (rapid Romanowsky, ×40). B, FNAB specimen in CMF. Highly cellular smear containing many mononuclear cells with smudged condensed chromatin and associated cytoplasm having a stellate, polygonal, or spindle shape (Papanicolaou, ×20). Note the multinucleated giant cells (inset, Papanicolaou, ×20). C, Low-power view of corresponding biopsy specimen showing characteristic histologic features of CMF with a distinctive lobular configuration. There are extensive myxomatous and chondroid areas with condensation of the stromal cells toward the periphery (H&E, ×4). D, High-power view of biopsy specimen showing the central chondromyxoid portions of the lobules containing spindle-shaped or stellate cells with bland nuclear features (H&E, ×40).

Image 1

(Case 1) A, Fine-needle aspiration biopsy (FNAB) specimen in chondromyxoid fibroma (CMF). Hypercellular chondromyxoid fragments containing ovoid cells with smudged nuclear features in a chondromyxoid stoma (rapid Romanowsky, ×40). B, FNAB specimen in CMF. Highly cellular smear containing many mononuclear cells with smudged condensed chromatin and associated cytoplasm having a stellate, polygonal, or spindle shape (Papanicolaou, ×20). Note the multinucleated giant cells (inset, Papanicolaou, ×20). C, Low-power view of corresponding biopsy specimen showing characteristic histologic features of CMF with a distinctive lobular configuration. There are extensive myxomatous and chondroid areas with condensation of the stromal cells toward the periphery (H&E, ×4). D, High-power view of biopsy specimen showing the central chondromyxoid portions of the lobules containing spindle-shaped or stellate cells with bland nuclear features (H&E, ×40).

Immunohistochemical analysis is generally not useful in distinguishing CMF from these other entities; however, recent studies have demonstrated a chromosomal abnormality [inv(6)(p25q13) pericentromeric inversion] that is a useful marker for chondromyxoid fibroma.14

Image 2

(Case 2) A, Fine-needle aspiration biopsy (FNAB) specimen in chondromyxoid fibroma (CMF). Hypercellular chondromyxoid fragments (HCFs) consisting of stellate to spindle-shaped cells with bland nuclear features embedded in a bright metachromatic chondromyxoid stroma (rapid Romanowsky stain, ×40). B, FNAB specimen in CMF. Corresponding HCFs with bland stellate to spindle-shaped cells in a more fibrous chondromyxoid stroma (Papanicolaou, ×20). C, Corresponding low-power view of biopsy specimen showing a lobular configuration of chondromyxoid areas with condensation of the stromal cells toward the periphery (H&E, ×10). D, Corresponding high-power view of biopsy specimen showing chondromyxoid lobules containing spindle-shaped or stellate cells with bland nuclear features (H&E, ×40).

Image 2

(Case 2) A, Fine-needle aspiration biopsy (FNAB) specimen in chondromyxoid fibroma (CMF). Hypercellular chondromyxoid fragments (HCFs) consisting of stellate to spindle-shaped cells with bland nuclear features embedded in a bright metachromatic chondromyxoid stroma (rapid Romanowsky stain, ×40). B, FNAB specimen in CMF. Corresponding HCFs with bland stellate to spindle-shaped cells in a more fibrous chondromyxoid stroma (Papanicolaou, ×20). C, Corresponding low-power view of biopsy specimen showing a lobular configuration of chondromyxoid areas with condensation of the stromal cells toward the periphery (H&E, ×10). D, Corresponding high-power view of biopsy specimen showing chondromyxoid lobules containing spindle-shaped or stellate cells with bland nuclear features (H&E, ×40).

In the proper clinical and radiographic setting, the finding in hypocellular aspiration smears of HCFs may lead to the current diagnostic interpretation of CMF. This will permit an orthopedic surgeon to proceed to the operating room with confidence that a benign lesion with a low tendency for recurrence will be excised.

Image 3

(Case 3) A, Fine-needle aspiration biopsy (FNAB) specimen in chondromyxoid fibroma (CMF). Hypercellular chondromyxoid fragments (HCFs) with stellate to polygonal cells in a metachromatic chondromyxoid stroma (rapid Romanowsky, ×40). B, Corresponding HCF with spindle-shaped cells exhibiting bland nuclear features in a more fibrous chondromyxoid stroma (Papanicolaou, ×40). C, Corresponding biopsy specimen in CMF. Low-power view showing a focally lobulated proliferation of stellate to polygonal cells in a chondromyxoid stroma (H&E, ×10). D, Corresponding biopsy specimen in CMF. High-power view showing a focal condensation of stellate cells toward the periphery of a lobulated chondromyxoid stroma (H&E, ×40).

Image 3

(Case 3) A, Fine-needle aspiration biopsy (FNAB) specimen in chondromyxoid fibroma (CMF). Hypercellular chondromyxoid fragments (HCFs) with stellate to polygonal cells in a metachromatic chondromyxoid stroma (rapid Romanowsky, ×40). B, Corresponding HCF with spindle-shaped cells exhibiting bland nuclear features in a more fibrous chondromyxoid stroma (Papanicolaou, ×40). C, Corresponding biopsy specimen in CMF. Low-power view showing a focally lobulated proliferation of stellate to polygonal cells in a chondromyxoid stroma (H&E, ×10). D, Corresponding biopsy specimen in CMF. High-power view showing a focal condensation of stellate cells toward the periphery of a lobulated chondromyxoid stroma (H&E, ×40).

References

1.
Dahlin
DC
.
Chondromyxoid fibroma of bone, with emphasis on its morphological relationship to benign chondroblastoma
.
Cancer
 .
1956
;
9
:
195
203
.
2.
Schajowicz
F
Gallardo
H
.
Chondromyxoid fibroma (fibromyxoid chondroma) of bone: a clinico-pathological study of thirty-two cases
.
J Bone Joint Surg Br
 .
1971
;
53
:
198
216
.
3.
Rahimi
A
Beabout
J
Ivins
J
et al
Chondromyxoid fibroma: a clinicopathologic study of 76 cases
.
Cancer
 .
1972
;
30
:
726
736
.
4.
Unni
K
.
Dahlins Bone Tumors: General Aspects and Data on 11,087 Cases
 .
5th ed
.
Philadelphia, PA
:
Lippincott-Raven
;
1996
:
59
69
.
5.
Wu
CT
Inwards
CY
O’Laughlin
S
et al
Chondromyxoid fibroma of bone: a clinicopathologic review of 278 cases
.
Hum Pathol
 .
1998
;
29
:
438
446
.
6.
Layfield
LJ
Cytopathology of Bone and Soft Tissue Tumors
 .
New York, NY
:
Oxford University Press
;
2002
:
171
173
.
7.
Layfield
L
Ferreiro
J
.
Fine-needle aspiration cytology of chondromyxoid fibroma: a case report
.
Diagn Cytopathol
 .
1988
;
4
:
148
151
.
8.
Gupta
S
Dev
G
Marya
S
.
Chondromyxoid fibroma: a fine-needle aspiration diagnosis
.
Diagn Cytopathol
 .
1993
;
9
:
63
65
.
9.
Hazarika
D
Kumar
R
Rao
C
et al
Fine needle aspiration cytology of chondroblastoma and chondromyxoid fibroma: a report of two cases
.
Acta Cytol
 .
1994
;
38
:
592
596
.
10.
De Las Casas
LE
Singh
HK
Halliday
BE
et al
Myxoid chondrosarcoma of the sphenoid sinus and chondromyxoid fibroma of the iliac bone: cytomorphologic findings of two distinct and uncommon myxoid lesions
.
Diagn Cytopathol
 .
2000
;
22
:
383
389
.
11.
Estrada-Villasenor
E
Cedillo
ED
Martinez
GR
et al
Periosteal chondromyxoid fibroma: a case study using imprint cytology
.
Diagn Cytopathol
 .
2005
;
33
:
402
406
.
12.
Koh
J
Chung
J
Lee
S
et al
Chondrosarcoma of the proximal femur with myxoid degeneration mistaken for chondromyxoid fibroma in a young adult: a case report
.
Acta Cytol
 .
2001
;
45
:
254
258
.
13.
Keel
SB
Bhan
AK
Liebsch
NJ
et al
Chondromyxoid fibroma of the skull base: a tumor which may be confused with chordoma and chondrosarcoma: a report of three cases and review of the literature
.
Am J Surg Pathol
 .
1997
;
21
:
577
582
.
14.
Granter
SR
Renshaw
AA
Kozakewich
HP
et al
The pericentromeric inversion, inv (6)(p25q13), is a novel diagnostic marker in chondromyxoid fibroma
.
Mod Pathol
 .
1998
;
11
:
1071
1074
.

Upon completion of this activity you will be able to:

  • list the typical clinical and radiologic aspects of chondromyxoid fibroma.

  • describe the expected characteristics of an aspiration smear of chondromyxoid fibroma.

  • provide the cytologic differential diagnosis of chondromyxoid fibroma of bone.

The ASCP is accredited by the Accreditation Council for Continuing Medical Education to provide continuing medical education for physicians. The ASCP designates this educational activity for a maximum of 1 AMA PRA Category 1 Credit™ per article. This activity qualifies as an American Board of Pathology Maintenance of Certification Part II Self-Assessment Module.

The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose.

Questions appear on p 798. Exam is located at www.ascp.org/ajcpcme.