Direct Organogenesis and Plant Regeneration in Preconditioned Tissue Cultures of Lathyrus cicera L., L. ochrus (L.)DC. and L. sativus L.

Abstract

This study demonstrates the importance of preconditioning of source tissue in regeneration of multiple shoot buds from several species of Lathyrus. Preconditioned multiple shoots of Lathyrus cicera L., L. ochrus (L.) DC. and L. sativus L. were obtained by germinating seeds on Murashige and Skoog (MS) medium containing 50 μM N⁵-benzylaminopurine (BAP) for 2 to 3 weeks. Multiple shoot bud formation occurred when epicotyl explants of preconditioned shoots were cultured on MS medium containing 5–50 μM BAP. No shoot regeneration was observed from epicotyl explants which were obtained from non-preconditioned shoots. Shoot buds were formed directly on explants without an intervening callus phase after 2 to 3 weeks of culture. Regenerated shoot buds formed healthy shoots which developed prolific and strong roots when transferred to MS medium supplemented with 2.5 μM naphthaleneacetic acid (NAA).

Lathyrus cicera L., L. ochrus (L.) DC., Ochrus Vetch, L. sativus L., Lathyrus pea, de novo differentiation, epicotyl, preconditioning with BAP