Kinetics of the Ceramide Kinase Inhibitor K1, a Suppressor of Mast-Cell Activation

Abstract

In a previous study, we synthesized a novel inhibitor of ceramide kinase, K1. In this study, we determined that inhibition by K1 is non-competitive and that four intact six-membered rings are important to the inhibitory activity. Furthermore, we identified an effective in vivo concentration for K1, at which it did not influence any cellular lipid synthesis other than that of ceramide 1-phosphate (C1P) using RBL-2H3 cells, and found that K1 suppressed the activation of mast cells.

ceramide, ceramide kinase, ceramide 1-phosphate, sphingolipid