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Abstract

Staphylococcus aureus is an important opportunistic pathogen. It produces a variety of extracellular proteins, which may play important roles in infections by this bacterium. Staphyloccal α-toxin is a pore-forming 33-kDa polypeptide. In the first part of this article, we will refer to the roles of cell membranes in the pore formation by α-toxin as well as the molecular dissection of α-toxin for understanding its pore-forming nature. Staphylococcal γ-hemolysin and leukocidin are bi-component cytolysins, which have different cell specificities towards erythrocytes and leukocytes, respectively. We have found that these bi-component cytolysins share a common component. In the second part of this article, we will refer to the current status of knowledge of molecular cloning of the genes coding for γ-hemolysin and leukocidin, molecular domains of the toxins which decide the cell specificities, and mode of action of these bi-component toxins.

Staphylococcus aureus, pore-forming toxins, α-hemolysin, γ-hemolysin, leukocidin

References

J. N. Sheagren, New Engl. J. Med., 310, 1437–1442 (1984).

T. Wadstrom, Zbl. Bakt. Hyg. A., 266, 191–211 (1987).

A. C. MacCartney and J. P. Arbuthnott, in “Bacterial Toxins and Cell Membranes,” ed. by J. Jeljaszewicz and T. Wadstroem, Academic Press, London, 1978, pp. 89–127.

M. Rogolsky, Microbiol Rev., 43, 320–360 (1979).

S. Bhakdi and J. Tranum-Jensen, Microbiol. Rev., 55, 733–751 (1991).

P. Recsei, B. Kreiswirth, M. O’Reilly, P. Schlievert, A. Gruss, and R. Novick, Mol. Gen. Genet., 202, 58–61 (1985).

M. Kehoe, I. Duncan, T. Foster, N. Fairweather, and G. Dougan, Infect, Immun., 41, 1105–1111 (1983).

H. Ikigai and T. Nakae, Biochem. Biophys. Res. Commun., 130, 175–181 (1985).

N. Tobkes, B. A. Wallace, and H. Bayley, Biochemistry, 24, 1915–1920 (1985).

J. H. Freer, J. P. Arbuthnott, and A. W. Bernheimer, J. Bacteriol., 95, 1153–1168 (1967).

R. Fussle, S. Bhakdi, A. Sziegoleit, J. Tranum-Jensen, T. Kranz, and H. L. Wellensiek, J. Cell Biol., 91, 83–94 (1981).

G. Menestrina, J. Membrane Biol., 90, 177–190 (1985).

J. E. Gouaux, O. Braha, M. Hobaugh, L. Song, S. Cheley, C. Shustak, and H. Bayley, Proc. Natl. Acad. Sci. U.S.A., 91, 12828–12831 (1994).

B. Walker, M. Krishnasastry, and H. Bayley, J. Biol. Chem., 268, 5285–5292 (1993).

M. Palmer, R. Jursch, U. Weller, A. Valeva, K. Hilgert, M. Kehoe, and S. Bhakdi, J. Biol. Chem., 268, 11959–11962 (1993).

A. Valeva, A. Weisser, B. Walker, M. Kehoe, H. Bayley, S. Bhakdi, and M. Palmer, EMBO J., 15, 1857–1864 (1996).

B. Walker and H. Bayley, J. Biol. Chem., 270, 23065–23071 (1995).

M. Palmer, U. Weller, M. Messner, and S. Bhakdi, J. Biol. Chem., 268, 11963–11967 (1993).

T. Tomita, M. Watanabe, and Y. Yarita, Biochim. Biophys. Acta, 1145, 51–57 (1993).

A. Hildebrand, M. Pohl, and S. Bhadki, J. Biol. Chem., 266, 17195–17200 (1991).

M. Watanabe, T. Tomita, and T. Yasuda, Biochim. Biophys. Acta, 898, 257–265 (1987).

T. Tomita, M. Watanabe, and T. Yasuda, J. Biol. Chem., 267, 13391–13397 (1992).

T. Tomita, Μ. Watanabe, and T. Yasuda, Biochim. Biophys. Acta, 1104, 325–330 (1992).

H. Ikigai and T. Nakae, J. Biol. Chem., 262, 2156–2160 (1987).

P. N. Panton, M. C. Camb, F. C. O. Valentine, and M. R. C. P. Lond, Lancet, i, 506–508 (1932).

A. M. Woodin, Biochem. J., 75, 158–165 (1960).

V. Finck-Barbacon, G. Prevost, and Y. Piemont, Res. Microbiol., 142, 75–85 (1991).

M. Noda, T. Hirayama, I. Kato, and F. Matsuda, Biochem. Biophys. Acta, 633, 33–44 (1980).

G. P. Gladstone and W. E. Van Heyningen, Br. J. Exp. Pathol., 38, 123–137 (1957).

G. Prevost, G. Cribier, P. Couppie, P. Petiau, G. Supersac, V. Finck-barbancor, H. Monteil, and Y. Peimont, Infect. Immun., 63, 4121–4129 (1995).

A. Rahman, K. Izaki, I. Kato, and Y. Kamio, Biochem. Biophys. Res. Commun., 181, 138–144 (1991).

A. Rahman, H. Nariya, K. Izaki, I. Kato, and Y. Kamio, Biochem. Biophys. Res. Commun., 184, 640–646 (1992).

A. Rahman, K. Izaki, and Y. Kamio, Biosci. Biotech. Biochem., 57, 1234–1236 (1993).

J. Coony, Z. Kienle, T. J. Foster, and P. W. O’Toole, Infect. Immun., 61, 768–771 (1993).

F. Guyonnet and M. Plommet, Ann. Inst. Pasteur, 118, 19–33 (1970).

A. G. Taylor and A. W. Bernheimer, Infect. Immun., 10, 54–59 (1974).

H. Soboll, A. Ito, W. Schaeg, and H. Blobel, Zentralbl. Bakteriol. Hyg. Abt. Orig., 224, 184–193 (1973).

G. Supersac, G. Prevost, and Y. Peimont, Infect. Immun., 61, 580–587 (1993).

K. Sudo, W. Choorit, I. Asami, J. Kaneko, K. Muramoto, and Y. Kamio, Biosci. Biotech. Biochem., 59, 1786–1789 (1995).

W. Choorit, J. Kaneko, K. Muramoto, and Y. Kamio, FEBS Lett., 357, 260–264 (1995).

J. Kaneko, K. Muramoto, and Y. Kamio, Biosci. Biotech. Biochem., in press (1997).

G. Prevost, G. Supersac, D. A. Colin, P. Couppie, S. Sire, T. Hensier, P. Patiau, O. Meunier, B. Cribier, W. Konig, and Y. Piemont, in “Bacterial Protein Toxins,” Zbl. Bakt. Suppl. 24, ed. by Free et al., Gustav Fischer, New York, 1994, pp. 284–293.

Y. Kamio, A. Rahman, H. Nariya, T. Ozawa, and K. Izaki, FEBS Lett., 321, 15–18 (1993).

R. Mollby and T. Wadstrom, Infect. Immun., 3, 663–635 (1971).

H. Nariya, I. Asami, T. Ozawa, Y. Beppu, K. Izaki, and Y. Kamio, Biosci. Biotech. Biochem., 57, 2198–2199 (1993).

X. Wang, B. J. Wilkinson, and R. K. Jayaswal, Gene, 102, 105–109 (1991).

C. Y. Lee and J. J. Iandolo, J. Bacteriol., 170, 2409–2411 (1988).

G. Prevost, P. Couppie, P. Prevost, S. Gayet, P. Petiau, B. Cribier, H. Monteil, and Y. Piemont, J. Med. Microbiol, 42, 237–245 (1995).

M. Noda, I. Kato, T. Hirayama, and F. Matsuda, Infect. Immun., 29, 678–684 (1980).

H. Nariya, K. Izaki, and Y. Kamio, FEBS Lett., 329, 219–222 (1993).

T. Ozawa, J. Kaneko, H. Nariya, K. Izaki, and Y. Kamio, Biosci. Biotech. Biochem., 58, 602–605 (1994).

H. Nariya and Y. Kamio, Biosci. Biotech. Biochem., 59, 1603–1604 (1995).

R. Horton, H. Hunt, S. Ho, J. Pullen, and L. Pease, Gene, 77, 61–68 (1989).

D. A. Colin, I. Mazurier, S. Sire, and V. Finck-Barbancon, Infect. Immun., 62, 3184–3188 (1994).

T. Ozawa, J. Kaneko, and Y. Kamio, Biosci. Biotech. Biochem., 59, 1181–1183 (1995).

J. Kaneko, T. Tomita, and Y. Kamio, Biosci. Biotech. Biochem., 61, 846–851 (1997).

V. Finck-Barbancon, G. Duportail, O. Meunier, and D. A. Colin, Biochim. Biophys. Acta, 1182, 275–282 (1993).

N. Sugawara, T. Tomita, and Y. Kamio, FEBS Lett., submitted.

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