Mahendar Thudi is a Post-Doctoral Visiting Scientist, actively involved in development and application of genomic resources in chickpea and leading the activities in the Centre of Excellence in Genomics at the International Crops Research Institute for the Semi-Arid Tropics (ICRISAT) in India.
Scott Jackson is a Professor in plant genetics and genomics at the University of Georgia, Athens. His research has major focus on genomics of legumes and rice and, the exploration and utilization of genetic diversity in wild relatives of crop plants and on genome structure and evolution.
Gregory May is President of the National Centre for Genome Resource (NCGR) and Director of the New Mexico Genome Center, Santa Fe, New Mexico. He has extensive research experience in the area of genome structure and organization, gene targeting and DNA repair in plants.
Rajeev Varshney is Director, Centre of Excellence in Genomics at ICRISAT and Leader of Comparative and Applied Genomics Theme of CGIAR Generation Challenge Programe (GCP). His research has major focus on development and application of large-scale genomic and transcriptomic resources in legume crops by using next-generation sequencing and high-throughput genotyping technologies.
Mahendar Thudi, Yupeng Li, Scott A. Jackson, Gregory D. May, Rajeev K. Varshney; Current state-of-art of sequencing technologies for plant genomics research. Briefings in Functional Genomics 2012; 11 (1): 3-11. doi: 10.1093/bfgp/elr045
A number of next-generation sequencing (NGS) technologies such as Roche/454, Illumina and AB SOLiD have recently become available. These technologies are capable of generating hundreds of thousands or tens of millions of short DNA sequence reads at a relatively low cost. These NGS technologies, now referred as second-generation sequencing (SGS) technologies, are being utilized for de novo sequencing, genome re-sequencing, and whole genome and transcriptome analysis. Now, new generation of sequencers, based on the ‘next-next’ or third-generation sequencing (TGS) technologies like the Single-Molecule Real-Time (SMRT™) Sequencer, Heliscope™ Single Molecule Sequencer, and the Ion Personal Genome Machine™ are becoming available that are capable of generating longer sequence reads in a shorter time and at even lower costs per instrument run. Ever declining sequencing costs and increased data output and sample throughput for NGS and TGS sequencing technologies enable the plant genomics and breeding community to undertake genotyping-by-sequencing (GBS). Data analysis, storage and management of large-scale second or TGS projects, however, are essential. This article provides an overview of different sequencing technologies with an emphasis on forthcoming TGS technologies and bioinformatics tools required for the latest evolution of DNA sequencing platforms.