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Christina Eimer, Ashley D Sanders, Jan O Korbel, Tobias Marschall, Peter Ebert, ASHLEYS: automated quality control for single-cell Strand-seq data, Bioinformatics, 2021;, btab221, https://doi.org/10.1093/bioinformatics/btab221
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Abstract
Single-cell DNA template strand sequencing (Strand-seq) enables chromosome length haplotype phasing, construction of phased assemblies, mapping sister-chromatid exchange events and structural variant discovery. The initial quality control of potentially thousands of single-cell libraries is still done manually by domain experts. ASHLEYS automates this tedious task, delivers near-expert performance and labels even large data sets in seconds.
github.com/friendsofstrandseq/ashleys-qc, MIT license
Supplementary data are available at Bioinformatics online.