Abstract

Summary: The Dual Organellar GenoMe Annotator (DOGMA) automates the annotation of organellar (plant chloroplast and animal mitochondrial) genomes. It is a Web-based package that allows the use of BLAST searches against a custom database, and conservation of basepairing in the secondary structure of animal mitochondrial tRNAs to identify and annotate genes. DOGMA provides a graphical user interface for viewing and editing annotations. Annotations are stored on our password-protected server to enable repeated sessions of working on the same genome. Finished annotations can be extracted for direct submission to GenBank.

Availability:http://phylocluster.biosci.utexas.edu/dogma/

Supplementary information: Detailed documentation and tutorials for annotating both animal mitochondrial and plant chloroplast genomes can be found on the DOGMA home page.

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Author notes

1Department of Computer Sciences and 2Section of Integrative Biology, Institute of Cellular and Molecular Biology, University of Texas at Austin, Austin, TX 78712, USA and 3DOE Joint Genome Institute, 2800 Mitchell Drive, Walnut Creek, CA 94598, USA

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