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COVER: From “Serum- and Feeder-Free Culture of Mouse Germline Stem Cells” by Mito Kanatsu-Shinohara, Kimiko Inoue, Narumi Ogonuki, Hiroko Morimoto, Atsuo Ogura, and Takashi Shinohara. Biol Reprod 2011; 84:97-105. Published online ahead of print in BOR-Papers In Press September 15, 2010; doi:10.1095/ biolreprod.110.086462. Spermatogonial stem cells (SSCs) are the only stem cells in the body that transmit genetic information to the next generation. In this manuscript, authors established serum- and feeder-free culture conditions for SSCs. LacZ-expressing donor SSCs that had been cultured under serum- and feeder-free conditions reinitiated spermatogenesis following microinjection into seminiferous tubules of infertile recipient testis. Cover design and layout: Jane Tenenbaum, Tenenbaum Design, Cambridge, Massachusetts. Biology of Reproduction is printed with soy-based inks on acid-free paper by Allen Press, Inc., Lawrence, Kansas.