walnuts and sperm

... from baseline to 12 wk. Secondary endpoints included blood serum and sperm fatty acid (FA) profiles, sex hormones, and serum folate. The group consuming walnuts (n = 59) experienced improvement in sperm vitality, motility, and morphology, but no change was seen in the group continuing their usual diet...

.... These findings add to our knowledge of the mechanisms by which AR is induced in avian sperm, highlighting the similarities and differences between mammalian and avian sperm in terms of the behavior of the signal transduction events that precede AR. STAT Plus (AnalystSoft Inc., Walnut, CA, USA) was used...

... A model of water and solute transport based upon irreversible thermodynamics was used to determine murine sperm permeability parameters. Specifically, one pair of coupled nonlinear equations introduced by Kedem and Katchalsky [ 24 ] was used as the theoretical model of cell membrane permeability...

... in an estrogen-dependent manner [ 13 ], although the precise mechanism of its transcriptional regulation still remains controversial. OVGP1 has been shown to play an important role in enhancing sperm capacitation, sperm-zona binding, and penetration through the zona pellucida in several different species [ 13...

... Medical, Walnut Creek, CA) heating for 5 min in 0.01 M citrate buffer, pH 6.0, cooled for 10 min, rinsed in deionized water, treated for 10 min with 3% hydrogen peroxide to remove endogenous peroxidases, and incubated for 30 min with Superblock blocking buffer in PBS (Pierce Biotechnology, Rockford, IL...

... (SuperArray) were prehybridized in GEAprehyb (SuperArray) with heat-denatured sheared salmon sperm for 2 h at 60°C. Labeled cDNA probes were hybridized overnight at 60°C with continuous agitation. Following repetitive washing in saline-sodium citrate/SDS, hybridized cDNA probes were detected...

... , 27 ]. The estrous cycles of the female rats were monitored by daily evaluation of vaginal cytology in late afternoon [ 28 ]. Female rats in proestrus or early estrus were mated, and mating was verified on the following morning by the presence of a vaginal plug, sperm in vaginal smears, or both...

... carried out at 50°C as described, using a buffer containing 50% formamide, 5× Denhardt solution, 5× SSPE (sodium phosphate, EDTA, NaCl), 0.1% SDS, 0.1 mg/ml denatured salmon sperm DNA, and 0.3 mg/ml yeast tRNA. The probes were synthesized by RT-PCR using mouse bone marrow cell RNA as a template...