
Contents
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4.1 Genome structure of Escherichia coli 4.1 Genome structure of Escherichia coli
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4.2 The Trp operon 4.2 The Trp operon
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4.3 A model of the Trp operon 4.3 A model of the Trp operon
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4.4 Roles of the negative feedbacks in the Trp operon 4.4 Roles of the negative feedbacks in the Trp operon
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4.5 The lac operon 4.5 The lac operon
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4.6 Experimental evidence and modelling of bistable behavior of the lac operon 4.6 Experimental evidence and modelling of bistable behavior of the lac operon
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4.7 A reduced model derived from the detailed lac operon network 4.7 A reduced model derived from the detailed lac operon network
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4.8 The challenge ahead: complexity of the global transcriptional network 4.8 The challenge ahead: complexity of the global transcriptional network
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References References
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Exercises Exercises
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4 Gene-regulatory networks: from DNA to metabolites and back
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Published:July 2008
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Abstract
DNA has at least three functions essential to life: (i) its chemistry provides a mechanism for replicating genes (via the Watson–Crick base pairing); (ii) in its long strands are nucleotide sequences containing information for producing proteins (using the universal genetic code); and (iii) DNA provides instructions on how a cell would respond or adapt to its environment for growth and survival. This chapter illustrates the third function using specific examples of gene-regulatory networks (GRNs). Topics discussed include the genome structure of Escherichia coli, the Trp operon, a model of the Trp operon, roles of the negative feedbacks in the Trp operon, the lac operon, experimental evidence and modelling of bistable behavior of the lac operon, a reduced model derived from the detailed lac operon network, and complexity of the global transcriptional network. Exercises are provided at the end of the chapter.
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