Poster session 2

Extract

Abstract

Introduction: We have previously demonstrated the ability of retinoic acid to regulate the expression the atrial specific markers AMHC-1 and Tbx5 during early cardiac chamber specification. However, the molecular mechanisms responsible for this process still remain unclear. At present, microRNAs represent a novel layer of complexity in the regulatory networks controlling gene expression during cardiovascular development.

Purpose: The aim of this work is to study the intrinsic mechanism involved in this signaling pathway, since miR-133 has demonstrated to be involved in other earliest cardiac developmental processes.

Methods: Our model is focused on developing chick at gastrula stages by in vitro electroporation of miR-133, a microRNA which is has been shown expressed at the level of linear cardiac tube.

Results: Our results show the miR-133 expression at the level of the primitive heart tube. Moreover, or work reveals that overexpression of miR-133 suppresses AMHC-1 and Tbx5 expression.

Conclusion: These data support that miR-133, a putative microRNA that targets RARB 3́UTR, regulates the early cardiac chamber specification via retinoic acid pathway.