Abstract

In prairie voles, primary sensory areas are dominated by neurons that respond to one sensory modality, but some neurons also respond to stimulation of other modalities. To reveal the anatomical substrate for these multimodal responses, we examined the connections of the primary auditory area + the anterior auditory field (A1 + AAF), the temporal anterior area (TA), and the primary visual area (V1). A1 + AAF had intrinsic connections and connections with TA, multimodal cortex (MM), V1, and primary somatosensory area (S1). TA had intrinsic connections and connections with A1 + AAF, MM, and V2. Callosal connections were observed in homotopic locations in auditory cortex for both fields. A1 + AAF and TA receive thalamic input primarily from divisions of the medial geniculate nucleus but also from the lateral geniculate nucleus (LGd), the lateral posterior nucleus, and the ventral posterior nucleus (VP). V1 had dense intrinsic connections and connections with V2, MM, auditory cortex, pyriform cortex (Pyr), and, in some cases, somatosensory cortex. V1 had interhemispheric connections with V1, V2, MM, S1, and Pyr and received thalamic input from LGd and VP. Our results indicate that multisensory integration occurs in primary sensory areas of the prairie vole cortex, and this may be related to behavioral specializations associated with its niche.

Introduction

The use of the comparative approach allows us to understand the relationship between brains and behaviors that studies of a single mammal alone cannot reveal and to appreciate fundamental patterns of cortical organization common to all groups as well as the derivations that have evolved only in some groups. For instance, we can compare the organization of somatosensory cortex in different mammals and relate cortical magnification of representations of body parts such as the nose of star nose moles (Catania and Kaas 1995; Catania 2005), the bill of the platypus (Krubitzer, Manger, et al. (1995), and the hands of primates (Penfield and Rasmussen 1968; Pubols BH and Pubols LM 1971; Nelson et al. 1980; Padberg et al. 2007) to distinct morphological specializations. These cortical and morphological specializations are associated with unique behaviors such as feeding (Catania and Remple 2004), navigating and prey capture (Pettigrew et al. 1998), and dexterity (Wing et al. 1996; Wiesendanger 1999; Mountcastle 2005). For the auditory cortex (AC; for abbreviations, see Table 1), unique features of organization have been described in echolocating bats and humans. Echolocating bats have very distinct auditory areas that process highly specialized stimulus patterns, such as FM–FM (frequency modulation) sweeps (Suga et al. 1987), whereas human auditory cortex is specialized for processing auditory stimuli associated with language (Naatanen et al. 1997; Neville et al. 1998). Although the details of a particular specialization are unique to an individual species, the manner in which the brain is altered in evolution (e.g., more areas, larger areas, alterations in connectivity) appears to be conserved across mammals. Although comparative studies have demonstrated the strong relationship between cortical organization and peripheral specializations, there are fewer studies that examine the relationship between cortical organization and the constellation of behaviors that define a particular niche (Catania and Remple 2004).

Table 1

Abbreviations Table

Cortical fields 
1, somatosensory area in anterior parietal cortex 
2, somatosensory area in anterior parietal cortex 
3a, somatosensory area in anterior parietal cortex 
3b, primary somatosensory area 
A1, primary auditory area 
AAF, anterior auditory area 
AC, auditory cortex 
M, mean 
M1, primary motor area 
MM, multimodal cortex 
OB, olfactory bulb 
Oc1, occipital area 1 
Oc2, occipital area 2 
OTc, occipital temporal area, caudal division 
OTr, occipital temporal area, rostral division 
PM, parietal medial area 
PV, parietal ventral area 
Pyr, pyriform cortex 
R, rostral auditory field 
S1, primary somatosensory area 
S2, second somatosensory area 
TA, temporal anterior area 
Te1, temporal area 1 (Brodmann's area 41) 
Te2, temporal area 2 
Te3, temporal area 3 
TP, Temporal posterior architectonic area 
V1, primary visual area (Brodmann's area 17) 
V2, second visual area (Brodmann's area 18) 
VAF, ventral auditory area 
Thalamic nuclei 
AD, anterodorsal nucleus 
AM, anteromedial nucleus 
APT, anterior pretectal nucleus 
AV, anteroventral nucleus 
cp, cerebral peduncle 
eml, external medullary lamina 
Hb, habenular nucleus 
ic, internal capsule 
IGL, intergeniculate leaflet 
LD, lateral dorsal nucleus 
LGd, lateral geniculate nucleus, dorsal division 
LGv, lateral geniculate nucleus, ventral division 
LP, lateral posterior nucleus 
MGC, medial geniculate complex 
MGd, medial geniculate nucleus, dorsal division 
MGm, medial geniculate nucleus, magnocellular division 
MGv, medial geniculate nucleus, ventral division 
opt, optic tract 
PAG, periaqueductal gray 
pc, posterior commissure 
Pli, posterior limitans thalamic nucleus 
PO, posterior nucleus 
PP, peripeduncular nucleus 
Rt, reticular nucleus 
SC, superior colliculus 
SG, suprageniculate 
SNR, substantia nigra 
VL, ventral lateral nucleus 
VP, ventral posterior nucleus 
VPl, ventral posterior nucleus, lateral division 
VPm, ventral posterior nucleus, medial division 
ZI, zona incerta 
Cortical fields 
1, somatosensory area in anterior parietal cortex 
2, somatosensory area in anterior parietal cortex 
3a, somatosensory area in anterior parietal cortex 
3b, primary somatosensory area 
A1, primary auditory area 
AAF, anterior auditory area 
AC, auditory cortex 
M, mean 
M1, primary motor area 
MM, multimodal cortex 
OB, olfactory bulb 
Oc1, occipital area 1 
Oc2, occipital area 2 
OTc, occipital temporal area, caudal division 
OTr, occipital temporal area, rostral division 
PM, parietal medial area 
PV, parietal ventral area 
Pyr, pyriform cortex 
R, rostral auditory field 
S1, primary somatosensory area 
S2, second somatosensory area 
TA, temporal anterior area 
Te1, temporal area 1 (Brodmann's area 41) 
Te2, temporal area 2 
Te3, temporal area 3 
TP, Temporal posterior architectonic area 
V1, primary visual area (Brodmann's area 17) 
V2, second visual area (Brodmann's area 18) 
VAF, ventral auditory area 
Thalamic nuclei 
AD, anterodorsal nucleus 
AM, anteromedial nucleus 
APT, anterior pretectal nucleus 
AV, anteroventral nucleus 
cp, cerebral peduncle 
eml, external medullary lamina 
Hb, habenular nucleus 
ic, internal capsule 
IGL, intergeniculate leaflet 
LD, lateral dorsal nucleus 
LGd, lateral geniculate nucleus, dorsal division 
LGv, lateral geniculate nucleus, ventral division 
LP, lateral posterior nucleus 
MGC, medial geniculate complex 
MGd, medial geniculate nucleus, dorsal division 
MGm, medial geniculate nucleus, magnocellular division 
MGv, medial geniculate nucleus, ventral division 
opt, optic tract 
PAG, periaqueductal gray 
pc, posterior commissure 
Pli, posterior limitans thalamic nucleus 
PO, posterior nucleus 
PP, peripeduncular nucleus 
Rt, reticular nucleus 
SC, superior colliculus 
SG, suprageniculate 
SNR, substantia nigra 
VL, ventral lateral nucleus 
VP, ventral posterior nucleus 
VPl, ventral posterior nucleus, lateral division 
VPm, ventral posterior nucleus, medial division 
ZI, zona incerta 

Prairie voles provide an ideal system to examine the relationship between a unique niche and specializations in cortical organization. Unlike most rodents and most mammals, they are socially monogamous and live in small social groups in which pair-bonded animals mate for life and practice alloparenting (Carter and Getz 1993), and much of their social interactions rely on vocalizations.

Rodent vocalizations have been examined in a number of contexts from pup isolation to adult mating with functional hypotheses for vocalizations from acoustic by-product to a communicatory signal has been postulated (Blumberg and Sokoloff 2001; Ehret 2005; Portfors 2007). Despite the fact that the exact function of rodent vocalizations is still debated, neuronal responses to species-specific calls in the inferior colliculus (IC), medial geniculate body, and auditory cortex of the guinea pig have been demonstrated (Suta et al. 2008), and specific frequencies of rat vocalizations have been linked with specific types of stimuli. For example, the 22 kHz vocalization of rats has been linked in adults and pups with aversive stimuli and the 50 kHz vocalization with positive stimuli (for review, see Portfors 2007). Therefore, vocalizations likely play a larger role in rodent social behavior than previously accepted. Although few studies have examined vocalizations in prairie voles, studies have demonstrated that compared with a number of other rodents prairie voles depend a great deal on audition for mating and parent/offspring interactions (Lepri et al. 1988; Shapiro and Insel 1990; Rabon et al. 2001).

In a previous study, we found that this reliance on audition for important social behaviors was reflected in the amount of cortex devoted to auditory processing (Fig. 1).

Figure 1.

(A) Composite reconstruction of flattened vole cortex illustrating the distribution of neuronal responses from electrophysiological recording sites and their relationship to architectonically defined cortical areas. Neurons in S1 responded primarily to somatosensory stimulation, neurons in V1 responded mostly to visual stimulation, and neurons in auditory cortex responded mostly to auditory stimulation. However, multisensory responses can clearly be distinguished within these primary areas. When neurons in primary areas and MM responded to more than one modality of stimulation, 93% of the responses were to auditory stimulation plus either visual or somatosensory stimulation. Purple dots represent sites in which neurons responded to somatosensory stimuli. Yellow dots represent sites in which neurons responded to visual stimuli. Green dots represent sites in which neurons responded to auditory stimuli. Black dots represent sites in which neurons responded to more than one modality of stimulation. Thin lines in S1 denote body part representation boundaries. (B) Light field, digital image of cortex that has been flattened, sectioned parallel to the cortical surface, and stained for myelin from a different case than that illustrated in (A). Although the entire series of sections were used to draw borders, architectonically distinct areas can be visualized even in this single section. Medial is to the top, and caudal is to the right. Fp, forepaw; tr, trunk; vib, vibrissae. Other abbreviations are found in Table 1.

Figure 1.

(A) Composite reconstruction of flattened vole cortex illustrating the distribution of neuronal responses from electrophysiological recording sites and their relationship to architectonically defined cortical areas. Neurons in S1 responded primarily to somatosensory stimulation, neurons in V1 responded mostly to visual stimulation, and neurons in auditory cortex responded mostly to auditory stimulation. However, multisensory responses can clearly be distinguished within these primary areas. When neurons in primary areas and MM responded to more than one modality of stimulation, 93% of the responses were to auditory stimulation plus either visual or somatosensory stimulation. Purple dots represent sites in which neurons responded to somatosensory stimuli. Yellow dots represent sites in which neurons responded to visual stimuli. Green dots represent sites in which neurons responded to auditory stimuli. Black dots represent sites in which neurons responded to more than one modality of stimulation. Thin lines in S1 denote body part representation boundaries. (B) Light field, digital image of cortex that has been flattened, sectioned parallel to the cortical surface, and stained for myelin from a different case than that illustrated in (A). Although the entire series of sections were used to draw borders, architectonically distinct areas can be visualized even in this single section. Medial is to the top, and caudal is to the right. Fp, forepaw; tr, trunk; vib, vibrissae. Other abbreviations are found in Table 1.

Compared with many other rodents and similarly sized mammals, auditory cortex in the prairie vole occupies a larger percentage of the cortical sheet (Campi et al. 2007). In addition to cortical areas primary auditory area and anterior auditory field (A1 + AAF) and temporal anterior area (TA), neurons responsive to auditory stimulation are located in abundance in multimodal cortex (MM) surrounding A1 + AAF and TA and can even be found within the primary somatosensory area (S1) and the primary visual area (V1) (Campi et al. 2007). Specifically, in V1, about a quarter of all recording sites had neurons responsive to multimodal stimulation, with all these sites containing neurons responsive to auditory stimulation and stimulation of some other modality such as vision (86%). We proposed that this increased multisensory processing observed in primary cortical fields as well as the predominance of auditory responses (93%) for multimodal neurons in S1, V1, and MM is associated with the importance of the auditory system in social interactions. These findings prompted us to ask how these behavioral and physiological specializations would be reflected in the corticocortical and thalamocortical connections in prairie voles and if the connection patterns of auditory and visual areas were different than those of nonmonogamous rodents that relied less on the auditory system for important social interactions.

The goal of the present study was to determine the source of inputs to A1 + AAF, TA, and V1. Cortical injections of anatomical tracers were placed in these cortical fields and labeled cells, and axon terminals in the neocortex and thalamus were related to architectonic boundaries (Fig. 1B). We compare these data with findings from other rodents that have different social systems and niches to better understand the types of cortical organization changes that may accompany these differences.

Materials and Methods

A total of 10 cortical injections were placed in 10 adult prairie voles (Microtus ochrogaster), and of these, 5 injections were placed in A1 + AAF in 5 animals, 2 injections were placed in TA in 2 animals, and 3 injections were placed in V1 in 3 animals. All procedures were approved by the Internal Animal Care and Use Committee and conformed to National Institutes of Health guidelines.

Neuroanatomical Tracer Injections

The neuroanatomical tracer injections were performed under standard sterile conditions. At the beginning of these experiments, the animals were anesthetized with intramuscular injections of ketamine hydrochloride (67 mg/kg) and xylazine (13 mg/kg). Supplemental doses were given as needed to maintain a surgical plane of anesthesia. Once anesthetized, the skin was cut, the skull was exposed, and a craniotomy was made over a small area of the cortex. A 10% solution of 10 000 mw each of biotinylated dextran amine (BDA), fluororuby (FR), or fluoroemerald (FE; Invitrogen, Carlsbad, CA) was injected with either a calibrated Hamilton syringe (Hamilton Co., Reno, NV) or a glass pipette attached to a picospritzer (General Valve Corp., Fairfield, NJ). Approximately 0.4 μL of the tracer was injected at depth of 400–600 μm in cortex. This quantity produced small injections averaging 450 μm in diameter as measured in flattened cortical sections with tracer deposit in all 6 layers (Fig. 2A,E–H). Only cases that were restricted to the cortical layers and did not invade underlying structures were used (Fig. 2E–H).

Figure 2.

Digital image of the injection of BDA in A1 + AAF from case 07-36 (A) and labeled cells in MM cortex resulting from this injection (B). The digital image in (C) is a thalamic section from case 07-111 reacted for CO + BDA. In this case, TA was injected and labeled axons can be seen in MG. The digital image in (D) is a thalamic section from case 07-121 reacted for CO + BDA in which labeled cells in MG can be seen. (EH) Digital images of injection site of FR in A1 + AAF in case 08-108. This cortex has been flattened and cut parallel to the cortical surface. Different cortical depths are imaged to demonstrate that the injection site did not invade underlying structures. The digital image in (E) is the most superficial section, (F) Is from middle cortical layers, and (G, H) are of the deepest section showing the hippocampus coming in to view in the lower left corner. Medial is to the top, and rostral is to the left in images (A, B). Dorsal is to the top, and medial is to the right in (C and D). Medial is to the top, and rostral is to the right in images (EH). Conventions as in previous figure. For list of abbreviations, see Table 1.

Figure 2.

Digital image of the injection of BDA in A1 + AAF from case 07-36 (A) and labeled cells in MM cortex resulting from this injection (B). The digital image in (C) is a thalamic section from case 07-111 reacted for CO + BDA. In this case, TA was injected and labeled axons can be seen in MG. The digital image in (D) is a thalamic section from case 07-121 reacted for CO + BDA in which labeled cells in MG can be seen. (EH) Digital images of injection site of FR in A1 + AAF in case 08-108. This cortex has been flattened and cut parallel to the cortical surface. Different cortical depths are imaged to demonstrate that the injection site did not invade underlying structures. The digital image in (E) is the most superficial section, (F) Is from middle cortical layers, and (G, H) are of the deepest section showing the hippocampus coming in to view in the lower left corner. Medial is to the top, and rostral is to the left in images (A, B). Dorsal is to the top, and medial is to the right in (C and D). Medial is to the top, and rostral is to the right in images (EH). Conventions as in previous figure. For list of abbreviations, see Table 1.

Histological Processing

Tracers were allowed to transport for 6 days with the exception of case 07-136 in which 14 days of transport were allowed, after which time the animals were euthanized with a lethal dose of sodium pentobarbital (250 mg/kg). The animals were perfused transcardially with 0.9% saline, followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB, pH 7.4), and 4% paraformaldehyde in 10% sucrose in PB. After fixation, the brain was extracted from the skull; the hemispheres were separated from the thalamus, weighed, and then flattened between 2 glass slides. The cortex and thalamus were immersed in 30% sucrose overnight. The cortex was sectioned tangential to the cortical surface at a 20 μm thickness on a freezing microtome. This preparation allows the overall organization and positions of fields relative to each other to be determined. The thalamus and brainstem were sectioned coronally at a 40 μm thickness on a freezing microtome. In all cases, alternate series of cortical sections were reacted for myelin (Fig. 1B; Gallyas 1979; Fang et al. 2005; Padberg et al. 2005; Campi et al. 2007), processed for both cytochrome oxidase (CO; Carroll and Wong-Riley 1984) and BDA (Fig. 2A–D), using standard avidin–biotin development (Vectastain Elite; Vector Laboratories, Burlingame, CA), or mounted for fluorescence microscopy. Alternate series of sections in the thalamus were processed for Nissl, processed for combined CO + BDA, or mounted for fluorescence microscopy to relate cytoarchitectonic borders to labeled cell bodies and axon terminals. The CO + BDA reaction was a 2-step process in which the sections were first processed for CO using cytochrome C and diaminobenzidine (DAB). Second, BDA labeling was visualized with an avidin–biotin complex procedure followed by a metal-enhanced DAB reaction (Veenman et al. 1992).

Data Analysis and Reconstruction

Labeled cells, axon terminals, and injections sites were marked using a camera lucida for sections stained for CO/BDA and an X/Y stage encoding system for alternate sections mounted for fluorescence microscopy (MD Plot 5.2; AccuStage, Shoreview, MN). Every section in each of these series was reconstructed. Individual reconstructions of labeled cells were combined into a composite. Architectonic boundaries of adjacent sections were traced using a camera lucida (Stemi SV6; Carl Zeiss, Germany), The entire series of sections were used to reconstruct architectonic boundaries. The composites for the cells/terminals and architectonic boundaries were combined into a final summary display (e.g., Fig. 4A).

Thalamic sections were reconstructed as above; however, labeled cells were directly related to architectonic boundaries for each section. Specifically, individual drawings of each thalamic section were made delineating labeled cells and axon terminals and architectonic borders. These sections were then assembled into a series as seen in Figures (Figs 8– 10 and 12 and 13).

The total number of labeled cells was counted for the cortex and then for the thalamus, and the percentage of labeled cells present in each cortical area or thalamic nucleus was calculated (i.e., labeled cells per cortical area/total cortical labeled cells × 100).

Glass-mounted sections were scanned at 20× (0.46 μm/pixel) in an Aperio ScanScope T3 scanner (Aperio Technologies, Vista, CA) for use in figures. Minimal alterations using Adobe Photoshop were made in the brightness and contrast for all photomicrographs.

Results

The goal of the current study was to determine the connections of auditory and visual cortex to uncover the anatomical substrate for multisensory processing that we previously observed in our functional mapping studies of primary sensory areas of the prairie vole (Campi et al. 2007).

Determination of Cortical Field Boundaries

Results from our previous study in the prairie vole are critical for identifying cortical field boundaries, localizing our injection sites in the neocortex, and interpreting the present results. Electrophysiologically determined boundaries and their relation to myeloarchitectonic boundaries are briefly described here for the prairie vole (Figs 1 and 3A), and these are related to myeloarchitectonically determined boundaries in other commonly used rodents including mice (Fig. 3B), rats (Fig. 3C), and squirrels (Fig. 3D). Electrophysiological recording techniques allowed us to determine the organization of visual, somatosensory, and auditory cortex in prairie voles (Fig. 1A) and relate distinct cortical fields to myeloarchitectonic boundaries. The topographic organization of S1 was determined and related to a densely myelinated field (Fig. 1B) that is similar in appearance and location to that described for S1 in other rodents such as squirrels (Sur et al. 1978; Krubitzer et al. 1986; Wong and Kaas 2008), rats (Welker 1971; Chapin and Lin 1984; Remple et al. 2003), and mice (Woolsey 1967; Nussbaumer and Van der Loos 1985; Hunt et al. 2006) and other mammals (e.g., Felleman et al. 1983; Jain et al. 1998; Catania et al. 2000; for review, see Kaas 1983; Karlen and Krubitzer 2007; Krubitzer and Campi 2009). A smaller, moderately myelinated field was identified caudolateral to S1, and neurons in this field had large receptive fields on the body. This field corresponds to the S2/PV (second somatosensory area and the parietal ventral area) region described in other rodents (Krubitzer et al. 1986; Hunt et al. 2006; Benison et al. 2007) and other mammals (e. g. Krubitzer and Calford 1992; Krubitzer et al. 1995; Beck et al. 1996).

Figure 3.

Photomicrographs of myelin stained sections of flattened cortex with architectonic boundaries denoted with thin broken lines. (A) Prairie vole. (B) Mouse. (C) Rat. (D) Squirrel. Complete areal boundaries cannot be seen in a single section, and it should be noted that the entire series are used for drawing composite reconstruction boundaries. However, in some of these images, a number of cortical fields are readily identified even in a single section including S1, the auditory core region including A1 + AAF and TA and V1. Conventions as in previous figure. For list of abbreviations, see Table 1.

Figure 3.

Photomicrographs of myelin stained sections of flattened cortex with architectonic boundaries denoted with thin broken lines. (A) Prairie vole. (B) Mouse. (C) Rat. (D) Squirrel. Complete areal boundaries cannot be seen in a single section, and it should be noted that the entire series are used for drawing composite reconstruction boundaries. However, in some of these images, a number of cortical fields are readily identified even in a single section including S1, the auditory core region including A1 + AAF and TA and V1. Conventions as in previous figure. For list of abbreviations, see Table 1.

In voles, neurons at the occipital pole responded predominantly to visual stimulation (Fig. 1A), and this region of cortex was coextensive with a darkly myelinated area which corresponds to area 17 or V1 in other rodents (Hall et al. 1971; Caviness 1975; Wagor et al. 1980; Coogan and Burkhalter 1993) and other mammals (e. g. Hubel and Wiesel 1968; Allman and Kaas 1971; Payne 1993; Kahn et al. 2000; for review, see Lyon 2007). A second, lightly myelinated field in which neurons respond to visual stimulation was also identified in voles and corresponds to area 18 or the second visual area, V2 as described in other rodents (Hall et al. 1971; Tiao and Blakemore 1976; Wagor et al. 1980; Malach 1989) and other mammals (e. g. Allman and Kaas 1974; Tusa et al. 1979; Rosa et al. 1999).

In the temporal region of cortex in prairie voles, a large myelinated region was observed in which neurons responded primarily to auditory stimulation. This region was broken into a darkly myelinated region located medially and a moderately myelinated region located laterally (Fig. 3A). The medial region is similar in location and appearance to area 41 or temporal cortex area (Te1) as described architectonically in rats and mice, and these fields correspond to 2 functionally defined auditory fields, A1 and AAF in mice (Caviness 1975; Stiebler et al. 1997), Mongolian gerbils (Thomas et al. 1993: Budinger et al. 2006), and rats (e. g. Rutkowski et al. 2003; Kalatsky et al. 2005; Polley et al. 2007; Fig. 3). In squirrels, this region corresponds to A1 and to a rostral field termed R (Fig. 3D, Merzenich et al. 1976; Luethke et al. 1988), the latter of which may be homologous to AAF in other rodents. The moderately myelinated lateral region corresponds TA (Fig. 3D), as described in squirrels (Merzenich et al. 1976; Luethke et al. 1988), and neurons in TA in squirrels respond to auditory stimulation. In mice (Stiebler et al. 1997) and rats (e.g., Polley et al. 2007; see Discussion), this region has been shown to contain several functionally defined auditory areas, and its location and architectonic appearance are similar to TA in squirrels, voles, and rats (Fig. 3). In the present study, we used a combined nomenclature for cortical areas from several rodents (i.e., mice, rats, gerbils, and squirrels) which was derived from both electrophysiological recording studies and architectonic analysis. The myeloarchitectonic appearance of the major subdivisions of the neocortex (S1, S2/PV, V1, V2, A1 + AAF, TA) in all these species is remarkably similar and provides an accurate framework for comparisons across rodents and across mammals in general.

Cortical Connections of A1 + AAF and TA

In 5 cases (Fig. 4; 2 cases not shown), injections were placed in the auditory core region (A1 + AAF), and in 2 cases, 07-111 and 07-136 (Fig. 5) injections were placed in TA. Injection sites ranged in size from 300 to 800 μm in diameter as measured in flattened cortical sections.

Figure 4.

Patterns of ipsilateral and contralateral cortical connections resulting from injections in A1 + AAF. Ipsilateral connections are shown in (A, C, and E), and contralateral connections are shown in (B, D, and F). Black filled circles represent labeled cells, and light gray patches represent anterograde terminal fields resulting from the injections in A1 + AAF. The injection site is denoted by a gray patch with a black outline. Thin lines denote architectonic boundaries, and dashed lines denote approximate boundaries. Note that labeled cells are observed in other auditory areas, MM, and in V1 and V2. In cases 07-36 and 07-121, BDA was injected into A1 + AAF, and in case 08-108, FR was injected into A1 + AAF. Conventions as in previous figure. For list of abbreviations, see Table 1.

Figure 4.

Patterns of ipsilateral and contralateral cortical connections resulting from injections in A1 + AAF. Ipsilateral connections are shown in (A, C, and E), and contralateral connections are shown in (B, D, and F). Black filled circles represent labeled cells, and light gray patches represent anterograde terminal fields resulting from the injections in A1 + AAF. The injection site is denoted by a gray patch with a black outline. Thin lines denote architectonic boundaries, and dashed lines denote approximate boundaries. Note that labeled cells are observed in other auditory areas, MM, and in V1 and V2. In cases 07-36 and 07-121, BDA was injected into A1 + AAF, and in case 08-108, FR was injected into A1 + AAF. Conventions as in previous figure. For list of abbreviations, see Table 1.

Figure 5.

Patterns of ipsilateral and contralateral cortical connections resulting from injections in TA. Ipsilateral connections are shown in (A, C), and contralateral connections are shown in (B, D). Black filled circles represent labeled cells, and light gray patches represent anterograde terminal fields resulting from the injections in A1 + AAF. The injection site is denoted by a gray patch with a black outline. Thin lines denote architectonic boundaries, and dashed lines denote approximate boundaries. Labeled cells are mostly in other auditory areas but are also found in MM and V1. BDA was injected into TA in both cases. Conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 5.

Patterns of ipsilateral and contralateral cortical connections resulting from injections in TA. Ipsilateral connections are shown in (A, C), and contralateral connections are shown in (B, D). Black filled circles represent labeled cells, and light gray patches represent anterograde terminal fields resulting from the injections in A1 + AAF. The injection site is denoted by a gray patch with a black outline. Thin lines denote architectonic boundaries, and dashed lines denote approximate boundaries. Labeled cells are mostly in other auditory areas but are also found in MM and V1. BDA was injected into TA in both cases. Conventions as in previous figures. For list of abbreviations, see Table 1.

A1 + AAF Connections

In all cases in which tracer was injected into A1 + AAF, a large percentage of labeled cells (mean [M] = 49%, see Fig. 6A) were intrinsic to A1 + AAF (Fig. 4A,C,E), and in most cases, these labeled cells surrounded the injection site and filled much of the field. Connections of A1 + AAF with TA were present in all cases with a mean of 14% of labeled cells observed in this field. Most of the labeled cells in all cases clustered toward the medial portion of TA, at the border with A1 + AAF. Labeled cell bodies and terminations were generally overlapping.

Figure 6.

Histograms depicting the percentage of labeled cells in ipsilateral (A) and contralateral cortex (B) resulting from injections in A1 + AAF (dark gray) and TA (light gray). The percentage of labeled cells in the ipsilateral cortex (C) and contralateral (D) cortex resulting from injections in V1. For injections in auditory cortex, connections were mostly from cortical areas associated with auditory processing, but connections were also observed with nonauditory cortical areas such as V1, V2, and S1/S2. Although connections of V1 were mostly from cortical areas associated with visual processing, connections with nonvisual cortical areas such as A1+AAf, TA, and S1 were also observed. Conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 6.

Histograms depicting the percentage of labeled cells in ipsilateral (A) and contralateral cortex (B) resulting from injections in A1 + AAF (dark gray) and TA (light gray). The percentage of labeled cells in the ipsilateral cortex (C) and contralateral (D) cortex resulting from injections in V1. For injections in auditory cortex, connections were mostly from cortical areas associated with auditory processing, but connections were also observed with nonauditory cortical areas such as V1, V2, and S1/S2. Although connections of V1 were mostly from cortical areas associated with visual processing, connections with nonvisual cortical areas such as A1+AAf, TA, and S1 were also observed. Conventions as in previous figures. For list of abbreviations, see Table 1.

Connections of A1 + AAF with MM were also observed in all but one case, and the percentages of labeled cells varied between 16% and 36% with a mean of 20% (Fig. 6A). In some cases, a cluster of labeled cells was located ventral to TA (Fig. 4A,C); in no case did this cluster extend past the rhinal fissure into pyriform cortex (Pyr). There was also a cluster of moderate label just medial to A1 + AAF and lateral to V2, in MM. Finally, there was sparse labeling in cortex between S1 and AC.

A1 + AAF were also connected with V1 and/or V2 in 3 cases (Fig. 4A,C; case 07-55 not shown). Labeled cells and axon terminals in V1 and V2 were distributed across the middle portion of the fields and comprised a small percentage of all labeled cells in the cortex (M in V1 = 7% and M in V2 = 7%). Cases 07-36 and 07-121 (Fig. 4A,C), in which the injection was located nearly in the center of the A1 + AAF, had the highest percentage of label in V1, 22% and 10%, respectively. Case 07-55 (not shown), which was on the very lateral edge of A1 + AAF, had only a few labeled cells in V1 (3%).

Connections of A1 + AAF with somatosensory cortex (S1 and/or S2) were observed in 2 cases (07-121 and 08-108; Fig. 4C,E). In these cases, 2 patches of terminal labeling and labeled cells were located at the caudal edge of S1 (vibrissae representation). In case 07-121, a small percentage of labeled cells (<3%) were located in the caudolateral edge of S2 (snout/face representation). Because of the small number of cells labeled in this region, the percentage of label in S1 and S2 was combined for analysis.

Cortical Connections of TA

Injection sites in TA were located in the caudomedial (07-111; Fig. 5A) and rostrolateral (07-136; Fig. 5C) portions of this field. A large proportion of labeled cells resulting from these injections were observed in other portions of TA (M = 54%; Fig. 6A). A moderate percentage of labeled cells were observed in A1 + AAF (M = 18%), and these labeled cells were scattered throughout the field. As with A1 + AAF, MM cortex immediately lateral to TA and medial to A1 + AAF contained a moderate proportion of labeled cells (M = 28%). Finally, connections of TA with V2 were sparse (M = 5%), and connections with V1 were extremely sparse (M = 0.12%) and restricted to the caudolateral boundary of V1 in one case (07-136).

Callosal Connections of A1 + AAF and TA

Callosal connections of both A1 + AAF and TA were observed in all but one case and were mostly in homotopic locations of these fields in the contralateral hemisphere (Figs 4B,D,F and 5B,D). Connections were moderate to sparse in all cases. In all cases in which transported tracer was observed in the opposite hemisphere following injections in A1 + AAF, all or almost all but one of the labeled cells were in a homotopic location in A1 + AAF in the contralateral hemisphere (Fig. 4B,D,F), except for 1 case where label was observed in TA along the medial boundary and in cortex caudolateral to S2 (Fig. 4F). In the 2 cases in which tracer was injected into TA, all the labeled cells were in the homotopic location in TA of the contralateral hemisphere (Fig. 5B,D). Terminal labeling in TA and A1 + AAF in the opposite hemisphere was only observed in one case, 07-136 (Fig. 5D). This was a BDA injection with a transport time of 14 days, and this likely intensified the terminal labeling contralaterally.

Cortical Connections of V1

Ipsilateral Connections of V1

In 3 cases, 07-56 (Fig. 7A,B), 07-76 (Fig. 7C,D), and 07-121 (Fig. 7E,F) injections were placed in V1. Injection sites ranged in size from 200 to 900 μm in diameter. Two of the injection sites (cases 07-76 and 07-56) were located rostrally (Fig. 7E) and one more caudally in V1 (Fig. 7A,C). The representation of the visual hemifield in V1 has been demonstrated to be similar in all rodents and all mammals examined. The representation of the lower visual quadrant is located rostromedially in V1, the upper quadrant is represented caudolaterally, the horizontal meridian representation bisects the upper and lower quadrants, and the vertical meridian is represented on the lateral edge of V1 (Hubel and Wiesel 1968; Allman and Kaas 1971; e.g., Hall et al. 1971; Montero 1973; Wagor et al. 1980; Vidyasagar et al. 1992; Kahn et al. 2000). Although retinotopic mapping was not done in the prairie vole, we assume a similar representation to that of all other rodents and all other mammals for V1. Using this information, 2 of our injection sites (07-56 and 07-76) are located in the expected location of central vision and one injection site (07-121) is located in the expected location of the representation of peripheral visual field at the horizontal meridian.

Figure 7.

Patterns of ipsilateral and contralateral cortical connections resulting from injections in V1. Ipsilateral connections are shown in (A, C, and E), and contralateral connections are shown in (B, D, and F). Black filled circles represent labeled cells, and light gray patches represent anterograde terminal fields resulting from the injections in A1 + AAF. The injection site is denoted by a gray patch with a black outline. Thin lines denote architectonic boundaries, and dashed lines denote approximate boundaries. Note that labeled cells are observed in other visual areas but are also in MM auditory cortex and in 2 cases Pyr. In cases 07-56 and 07-76, FR was injected into V1, and in case 07-121, FE was injected into V1. Conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 7.

Patterns of ipsilateral and contralateral cortical connections resulting from injections in V1. Ipsilateral connections are shown in (A, C, and E), and contralateral connections are shown in (B, D, and F). Black filled circles represent labeled cells, and light gray patches represent anterograde terminal fields resulting from the injections in A1 + AAF. The injection site is denoted by a gray patch with a black outline. Thin lines denote architectonic boundaries, and dashed lines denote approximate boundaries. Note that labeled cells are observed in other visual areas but are also in MM auditory cortex and in 2 cases Pyr. In cases 07-56 and 07-76, FR was injected into V1, and in case 07-121, FE was injected into V1. Conventions as in previous figures. For list of abbreviations, see Table 1.

All 3 V1 injection cases showed intrinsic connections with V1 (M = 58%; Fig. 6C), although the density of these connections was different between the cases. In case 07-121, in which the injection site is nearer the representation of the peripheral horizontal meridian, 90% of labeled cell bodies were within V1. In contrast, in cases 07-56 and 07-76, in which the injection sites are nearer the expected location of the representation of the vertical meridian near central vision, 35% and 49%, respectively, of labeled cell bodies were within V1. Connections with V2 were observed in all cases, but the amount of labeled cells in this cortical area was relatively low (6%). Moderate connections with MM cortex were observed in cases 07-56 and 07-76 (26% and 24%, respectively). Sparse connections with MM were observed in case 07-121 (3%). Moderate to sparse connections with S1 were observed in cases 07-56 and 07-76 (10% and 3%, respectively). No connections with S1 were observed in case 07-121. Moderate connections with Pyr were observed in 2 cases 07-56 and 07-76 (15% and 13%, respectively). In all cases, V1 had sparse connections with auditory cortex (Fig. 6C). Thus, the connections of the central visual representation in V1 are more broadly distributed than the connections of the peripheral visual representation.

Callosal Connections of V1

For all cases, the densest callosal connections of V1 were with V1 of the opposite hemisphere (44%; Figs 6D and 7B,D,F). Injection sites at the rostrolateral border of V1 resulted in labeled cells mainly in the rostrolateral portion of V1 in the opposite hemisphere in a roughly homotopic location to that injected. The injection site at the caudal pole of V1 resulted in labeled cells mainly at the caudolateral border of V1 in the expected location of the vertical meridian of the upper visual quadrant (Fig. 7E). Moderate connections were also observed with V2 (15%), and these connections were scattered throughout the field in one case and more restricted in 2 cases (Fig. 7B,D,F). Light to moderate connections were observed with MM in 2 cases (11% and 29%, respectively; Fig. 6D), and sparse connections with MM cortex were observed in case 07-121 (3%). Moderate connections with S1 and Pyr of the opposite hemisphere were observed in case 07-76 (Fig. 7D). Finally, moderate to sparse connections were observed with A1 + AAF (12%) in all cases, but only sparse connections were observed with TA (4%) in 2 cases (Fig. 7B,F). As with ipsilateral cortical connection, interhemispheric connections of the central visual representation of V1 are more broadly distributed.

Architectonic Subdivisions of Thalamus

Subdivisions of the thalamus were delineated using sections stained for Nissl substance and CO (Fig. 8). Several nuclei can clearly be distinguished using these stains. The dorsal and ventral divisions of the lateral geniculate nucleus, LGN (LGd and LGv), stain darkly for CO and are separated by the lightly stained CO and cell sparse intergeniculate leaflet. Further, both LGd and LGv contained small, darkly stained cells in tissue stained for Nissl substance, but there were no discernable layers or subdivisions observed in either of these nuclei (Fig. 8A–F). Dorsal to the LGd, the lateral posterior nucleus (LP) reacted moderately for CO and had a medium cell packing density distinguished with Nissl staining (Fig. 8A–D). The lateral and medial divisions of LP could be distinguished from each other by darker CO reactivity and denser cell packing in the medial division. Medial to the LGd, VP can be seen as a triangular shaped nucleus that is darkly reactive for CO and has small, densely packed cells surrounded laterally by the cell sparse, lightly reactive (CO) external medullary lamina (Fig. 8A,B). The medial geniculate complex forms the caudal boundary of VP. The medial geniculate nucleus (MGN) can be divided into the dorsal, ventral, and medial divisions by cell packing density differences as well as differences in CO reactivity. The ventral division (MGv) is the largest, the most darkly reactive for CO, and the most densely packed of the MGN divisions. The dorsal division (MGd) is moderately reactive for CO with medium density cell packing. The medial or magnocellular division (MGm) is lightly reactive for CO with a sparse density of large cells. The optic tract (opt) is a lightly reactive (CO), cell sparse band on the lateral edge of each section, and the cerebral peduncle (cp) is a lightly reactive band on the ventral lateral edge of each section. The substantia nigra (SNR) is darkly reactive for CO with medium to sparse cell packing density distinguished by Nissl staining.

Figure 8.

Digital images of coronal sections through the prairie vole thalamus that have been reacted for CO (A, C, E, G, I) and stained for Nissl (B, D, F, H, J). This series is from rostral (A, B) through caudal sections (I, J) showing nuclei in the thalamus where labeled cells and terminals were identified. In rostral sections of the thalamus, VP, LGN, LD, LP, and PO can be readily identified in either or both CO and Nissl stains (A, B). At middle thalamic levels, the transition between VP, MGN, and LGN can be seen (CF). Further caudally, MGd, MGv, MGm, APT, SC, and PAG are distinct in either or both stains (GJ). Sections are 120 μm apart. Scale bar is 1 mm. Dorsal is to the top and lateral to the left. For list of abbreviations, see Table 1.

Figure 8.

Digital images of coronal sections through the prairie vole thalamus that have been reacted for CO (A, C, E, G, I) and stained for Nissl (B, D, F, H, J). This series is from rostral (A, B) through caudal sections (I, J) showing nuclei in the thalamus where labeled cells and terminals were identified. In rostral sections of the thalamus, VP, LGN, LD, LP, and PO can be readily identified in either or both CO and Nissl stains (A, B). At middle thalamic levels, the transition between VP, MGN, and LGN can be seen (CF). Further caudally, MGd, MGv, MGm, APT, SC, and PAG are distinct in either or both stains (GJ). Sections are 120 μm apart. Scale bar is 1 mm. Dorsal is to the top and lateral to the left. For list of abbreviations, see Table 1.

Thalamic Connections of Auditory Cortex

In the 5 cases in which injections were placed in A1 + AAF, the majority of thalamic input (M = 93%) was from 2 of the divisions of the MGN (Figs 9– 11). Specifically, 63% of cells projecting to A1 + AAF are from MGv (Fig. 11A), and 30% of the cells are from MGd. Interestingly, projections from nuclei associated with other sensory systems were also observed in 4 of the cases in which injections were restricted to A1 + AAF. For example, 3% of the labeled cells in the thalamus were located in the LGd in 3 cases (Figs 9– 11), 2% of the labeled cells were in LP in cases 07-36 and 08-108, and in case 07-121, 8% of labeled cells in the thalamus were in VP. The labeled cells were equally split between ventral posterior nucleus, medial division (VPm) and ventral posterior nucleus, lateral division (VPl). In this same case, labeled cells were also observed in S1 and S2 of the cortex. In case 07-36, terminal labeling was observed in MGd and MGv (Figs 2C and 9A).

Figure 9.

Reconstructions of a coronally cut series of sections through the thalamus for cases 07-36 (A) and 07-121 (B). In these cases, an injection of BDA was placed in A1 + AAF (Fig. 4). The resulting locations of labeled cell bodies relative to thalamic nuclei boundaries are shown. Black filled circles represent BDA-labeled cells, and gray patches represent anterograde terminal fields resulting from the BDA injection. Most of the labeled cells and anterograde terminals from the injections were observed within divisions of MGN. A small cluster of labeled cells was observed in LGd in cases 07-36 and 07-121. Labeled cells were also observed in VPl and VPm in case 07-121. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. In this and the following figure, the series of sections run from rostral (top left) through caudal (bottom right). Sections are 120 μm apart. Scale bar is 1 mm. Other conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 9.

Reconstructions of a coronally cut series of sections through the thalamus for cases 07-36 (A) and 07-121 (B). In these cases, an injection of BDA was placed in A1 + AAF (Fig. 4). The resulting locations of labeled cell bodies relative to thalamic nuclei boundaries are shown. Black filled circles represent BDA-labeled cells, and gray patches represent anterograde terminal fields resulting from the BDA injection. Most of the labeled cells and anterograde terminals from the injections were observed within divisions of MGN. A small cluster of labeled cells was observed in LGd in cases 07-36 and 07-121. Labeled cells were also observed in VPl and VPm in case 07-121. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. In this and the following figure, the series of sections run from rostral (top left) through caudal (bottom right). Sections are 120 μm apart. Scale bar is 1 mm. Other conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 10.

Reconstructions of a coronally cut series of sections through the thalamus for case 08-108. In this case, an injection of FR was placed in A1 + AAF (Fig. 4). The resulting locations of labeled cell bodies relative to thalamic nuclei boundaries are shown. Black filled circles represent FR-labeled cells. Most of the labeled cells from the injection were observed within MGv. A small cluster of labeled cells was observed in LGd/LP. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. Sections are 120 μm apart. Other conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 10.

Reconstructions of a coronally cut series of sections through the thalamus for case 08-108. In this case, an injection of FR was placed in A1 + AAF (Fig. 4). The resulting locations of labeled cell bodies relative to thalamic nuclei boundaries are shown. Black filled circles represent FR-labeled cells. Most of the labeled cells from the injection were observed within MGv. A small cluster of labeled cells was observed in LGd/LP. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. Sections are 120 μm apart. Other conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 11.

Histograms depicting the percentage of labeled cells in the dorsal thalamus resulting from injections in the auditory core regions (A) in A1 + AAF (dark gray) and TA (light gray) and in visual cortex (B). For injections in the A1 + AAF and TA, cells were mostly in thalamic nuclei associated with auditory processing. However, labeled neurons were also observed in nuclei of the thalamus associated with somatosensory processing (VP) and visual processing (LGd and LP). (B) Projections to V1 were mostly from thalamic nuclei associated with visual processing (B), connections with nonvisual nuclei of the thalamus associated with somatosensory processing (VP) and motor processing (VL) were also observed. For list of abbreviations, see Table 1.

Figure 11.

Histograms depicting the percentage of labeled cells in the dorsal thalamus resulting from injections in the auditory core regions (A) in A1 + AAF (dark gray) and TA (light gray) and in visual cortex (B). For injections in the A1 + AAF and TA, cells were mostly in thalamic nuclei associated with auditory processing. However, labeled neurons were also observed in nuclei of the thalamus associated with somatosensory processing (VP) and visual processing (LGd and LP). (B) Projections to V1 were mostly from thalamic nuclei associated with visual processing (B), connections with nonvisual nuclei of the thalamus associated with somatosensory processing (VP) and motor processing (VL) were also observed. For list of abbreviations, see Table 1.

Thalamic connections of TA were mainly with MGd (65%) in both cases, whereas MGv and MGm had a smaller percentage of labeled cells (Figs 11A and 12). In case 07-111, only a few labeled cells were observed in the 3 divisions of the MGN, whereas most of the labeling in these divisions was terminal labeling (Fig. 12A). There was also some terminal labeling in the LGd and VP in this case. The scarcity of labeled cells in this case was surprising because the injection in cortex included all cortical layers. In contrast, in case 07-136 (Fig. 12B), a large number of labeled cells were present in the 3 divisions of MGN with a small percentage of labeled cells in LP (5%) and sparse cell label in LGd and VP (see Figs 11A and 12B). The injection sites in these 2 cases differed in size and location, and this may account for some of the differences observed.

Figure 12.

Reconstructions of a coronally cut series of sections through the thalamus for cases 07-111 and 07-136. In these cases, an injection of BDA was placed in TA (Fig. 5). In case 07-111, most of the labeling was anterograde. A small cluster of cells was observed in divisions of MG. In case 07-136, most of the labeled cells were observed in MGd with some label in MGv. Sparser labeling was also observed in LG, LP, and VP. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. These sections are 120 μm apart. Other conventions as in previous figures. For list of abbreviations, see Table 1.

Figure 12.

Reconstructions of a coronally cut series of sections through the thalamus for cases 07-111 and 07-136. In these cases, an injection of BDA was placed in TA (Fig. 5). In case 07-111, most of the labeling was anterograde. A small cluster of cells was observed in divisions of MG. In case 07-136, most of the labeled cells were observed in MGd with some label in MGv. Sparser labeling was also observed in LG, LP, and VP. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. These sections are 120 μm apart. Other conventions as in previous figures. For list of abbreviations, see Table 1.

Thalamic Connections of V1

In the 2 cases (07-56 and 07-76) in which injection sites were rostral in V1, in the expected location of central vision, the majority of thalamic input (67%) comes from LGd (see Fig. 13 for reconstructions and Fig. 11B for percentages of connections). Moderate to sparse connections with LD (lateral dorsal nucleus; 7%), LP (21%), posterior thalamic group (PO; 14%), VP (13%), and ventral lateral nucleus (VL; 10%) were observed in case 07-76. In case 07-121 in which the injection site was located at the caudal pole of V1, the majority of input was from the LGd (55%) and moderate connections were from LD (18%). Moderate to sparse connections with PO (1%), VL (4%), and anteroventral nucleus (AV; 13%) were observed in case 07-121.

Figure 13.

Reconstructions of a coronally cut series of sections through the thalamus for cases 07-76 (A) and 07-121 (B). FR as placed in V1 in case 07-76 and FE was placed in V1 in case 07-121 (Fig. 7). The resulting locations of labeled cell bodies relative to thalamic nuclei boundaries are shown. Black filled circles represent FR or FE-labeled cells resulting from the injection. Most of the labeled cells from the injections were observed within LGd, LP, and LD. A small cluster of labeled cells was observed in VP and PO (A). Labeled cells were also observed in VL and in case 07-121 in AM, AV, and VA. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. Sections are 120 μm apart. Other conventions as in previous figure.

Figure 13.

Reconstructions of a coronally cut series of sections through the thalamus for cases 07-76 (A) and 07-121 (B). FR as placed in V1 in case 07-76 and FE was placed in V1 in case 07-121 (Fig. 7). The resulting locations of labeled cell bodies relative to thalamic nuclei boundaries are shown. Black filled circles represent FR or FE-labeled cells resulting from the injection. Most of the labeled cells from the injections were observed within LGd, LP, and LD. A small cluster of labeled cells was observed in VP and PO (A). Labeled cells were also observed in VL and in case 07-121 in AM, AV, and VA. Thin lines denote boundaries of thalamic nuclei, determined based on CO reactivity and Nissl stains. Sections are 120 μm apart. Other conventions as in previous figure.

Discussion

Cortical and Thalamic Connections of Auditory Cortex in Rodents

In the present investigation, we found that connections of A1 + AAF were most prominent with other regions of A1 + AAF, moderate with TA, and moderate with surrounding MM. The auditory core in the prairie vole corresponds to the architectonically defined area 41 or Te1 and is composed of 2 functional fields (A1 and AAF/R) as demonstrated in electrophysiological recording studies in mice (e.g., Stiebler et al. 1997), squirrels (e.g., Merzenich et al. 1976; Luethke et al. 1988), rats (e.g., Rutkowski et al. 2003; Polley et al. 2007), and Mongolian gerbils (Thomas et al. 1993; Budinger et al. 2006). All of our injections in the prairie vole were in the caudal or lateral portion of the field in the expected location of A1 rather than AAF. We did not observe any major differences in connections across injection sites in the present study suggesting that all of our injections were limited to a single field, A1. The pattern of connections in the present study is similar to that observed for the auditory core region (A1 + AAF/Te1) in other rodents such as Mongolian gerbils (Thomas and Lopez 2003; Budinger et al. 2006), squirrels (Luethke et al. 1988), and rats (e.g., Shi and Cassell 1997). In these other rodents, the connections of both fields in the auditory core region are fairly restricted to other auditory cortical areas. Interestingly, in the vole, direct connections of A1 + AAF were also observed with the V1 and the S1. Connections of A1 with S1 and visual areas were also reported in recent studies of the Mongolian gerbil (Budinger et al. 2006) but not in other rodents (Fig. 14A). In rats, cortex just dorsal to Te1 contained labeled cells following injections placed in Te1, and this region of cortex was defined as S1 (Shi and Cassell 1997). However, this location does not correspond to the location of S1 in rats as defined using electrophysiological recording techniques (e.g., Chapin and Lin 1984). Recently, connections of A1 with nonsensory areas including orbital, medial prefrontal, and cingulate areas of the cortex were observed in the Mongolian gerbil (Budinger et al. 2008). Connections with these cortical areas were not observed in the prairie vole. The possible significance of these differences in connection patterns between different rodents is discussed below.

Figure 14.

(A) Summary of A1 + AAF connections in 4 species rodents (A). Similar patterns of multisensory connections can be seen in the 2 socially monogamous rodents. These connections are not present in the promiscuous rodents. The AC of each rodent is represented by the elongated middle box. The boxes above are cortical area connections. The boxes below are thalamic nuclei connections. The thickness of the lines represents the relative density of connections. (B) Summary of V1 connections in 4 species of rodents. A pattern of multisensory connections can be seen in the Prairie vole. These connections are not present in the other rodents. V1 of each rodent is represented by the elongated middle box. The boxes above are cortical area connections. The boxes below are thalamic nuclei connections. The thickness of the lines represents the relative amount of connections. The connections of the Mongolian gerbil are from Budinger et al. (2006). The connections of the squirrel are from Kaas et al. (1972), Luethke et al. (1988), Kaas et al. (1990), and Wong et al. (2008). The connections of the rat are from Peters and Feldman (1976), Miller and Vogt (1984), Malach (1989), Shi and Cassell (1997), Winer et al. (1999), Kimura et al. (2003), and Polley et al. (2007). Connections from the mouse are from Caviness and Frost (1980), Simmons et al. (1982), and Wang and Burkhalter (2007). The design of this illustration was inspired by Figure 4 from Budinger et al. (2006). For list of abbreviations, see Table 1.

Figure 14.

(A) Summary of A1 + AAF connections in 4 species rodents (A). Similar patterns of multisensory connections can be seen in the 2 socially monogamous rodents. These connections are not present in the promiscuous rodents. The AC of each rodent is represented by the elongated middle box. The boxes above are cortical area connections. The boxes below are thalamic nuclei connections. The thickness of the lines represents the relative density of connections. (B) Summary of V1 connections in 4 species of rodents. A pattern of multisensory connections can be seen in the Prairie vole. These connections are not present in the other rodents. V1 of each rodent is represented by the elongated middle box. The boxes above are cortical area connections. The boxes below are thalamic nuclei connections. The thickness of the lines represents the relative amount of connections. The connections of the Mongolian gerbil are from Budinger et al. (2006). The connections of the squirrel are from Kaas et al. (1972), Luethke et al. (1988), Kaas et al. (1990), and Wong et al. (2008). The connections of the rat are from Peters and Feldman (1976), Miller and Vogt (1984), Malach (1989), Shi and Cassell (1997), Winer et al. (1999), Kimura et al. (2003), and Polley et al. (2007). Connections from the mouse are from Caviness and Frost (1980), Simmons et al. (1982), and Wang and Burkhalter (2007). The design of this illustration was inspired by Figure 4 from Budinger et al. (2006). For list of abbreviations, see Table 1.

Connections of TA in the present investigation were observed with A1 + AAF (mostly in the caudal portion of this field), with cortex caudal to and lateral to TA, and with V2. Electrophysiological recordings in this region demonstrate that neurons respond predominantly to auditory stimulation (Campi et al. 2007). Connections of TA in the squirrel (Luethke et al. 1988) were similar to those observed in the prairie vole but were somewhat more restricted in that no connections were observed with V2. In rats, the region of cortex that appears to correspond in location to TA in squirrels and prairie voles is composed of 2 fields termed the ventral auditory field (VA or VAF; e.g., Donishi et al. 2006; Kimura et al. 2007; Polley et al. 2007) and the suprarhinal auditory area (Polley et al. 2007). Cortical connections of VA(F) are predominantly with other auditory areas but are also observed with insular cortex and posterior parietal cortex (PPC; Kimura et al. 2007), which corresponds to cortex just dorsal to A1 + AAF and caudal to S1, which we term MM in prairie voles.

Callosal connections of both A1+AAF and TA were restricted to homotopic locations of either A1 + AAF or TA in all but one case. This is different than the patterns of callosal connections of A1 and TA in squirrels and Mongolian gerbils, which are most dense with matched locations in the opposite hemisphere but are also present with other auditory regions of the cortex (Luethke et al. 1988; Thomas and Lopez 2003). This was particularly true for the injection in TA in squirrels in which the injection in TA was relatively large and may have spread beyond the boundaries of the field (Luethke et al. 1988).

The pattern of thalamic connections of A1 + AAF in voles is similar to that seen in other rodents in that the majority of thalamic input to A1 + AAF (Te1) is from MGv, and the major input to TA (VAF) is from MGd in rats (e.g., Roger and Arnault 1989; Winer et al. 1999; Donishi et al. 2006), mice (Caviness and Frost 1980; Winer et al. 1999; Llano and Sherman 2008), squirrels (Wong et al. 2008), and other mammals such as cats (e.g., Morel and Imig 1987) and monkeys (e.g., de la Mothe et al. 2006). Thalamocortical connections of VAF, similar in location to our TA, were with both MGd and MGv (Kimura et al. 2007; Polley et al. 2007).

In the present study, we also observed projections to A1 + AAF and TA from VP, LGd, and LP. However, not all of these nuclei project to A1 + AAF and/or TA in all cases (Figs 10– 12). VP is associated with somatosensory processing, and LGd and LP are associated with visual processing. These connections have not been observed in rats or squirrels. However, connections with nonauditory nuclei including LP, LD, and posterior limitans thalamic nucleus associated with visual processing have been observed in the Mongolian gerbil (Budinger et al. 2006). The presence of multisensory inputs to primary auditory areas of the cortex in some rodents but not others raises the question of what generates this diversity in cortical organization and connectivity and what types of behaviors are subserved by a distinct type of processing network.

Cortical and Thalamic Connections of V1 in Rodents

In the present investigation, we found that connections of V1 were most prominent with other regions of V1 (area 17, occipital cortex area 1 or Oc1) and with V2 (area 18, Oc2, lateral visual areas), although the density of these connections in comparison to connections with other regions of cortex was different for presumptive central versus peripheral visual field representations (see Results, Connections of V1). This pattern of connections is similar to that observed in other rodents such as mice (Simmons et al. 1982; Wang and Burkhalter 2007), rats (Miller and Vogt 1984; Malach 1989; Coogan and Burkhalter 1993; Montero 1993), hamsters (Olavarria and Montero 1990), and squirrels (Kaas et al. 1989). Interestingly, in the vole, direct connections of V1 were also observed with the auditory cortex, the primary somatosensory cortex, and the Pyr (Fig. 14B). Connections of V1 with somatosensory and auditory areas of the cortex have not been reported in other rodents; however, connections of V1 have not been investigated in other monogamous rodents. Callosal connections of V1 in all cases tended to cluster around the representation of the vertical meridian as is commonly seen in other rodents (e.g., Thomas and Espinoza 1987; Kaas et al. 1989; Olavarria and Montero 1990; Wang and Burkhalter 2007) and other mammals. However, like ipsilateral connections, connections were more broadly distributed to other areas including A1 + AAF, TA, MM, and, in one case, Pyr. This pattern is not commonly observed in other rodents or other mammals. Thus, not only are connections of auditory cortex more broadly distributed in the prairie vole but connections of other primary areas, such as V1, are broadly distributed as well.

Thalamocortical connections of V1 are similar to those observed in other rodents in that the major thalamic input to V1 is from LGd (Nauta and Bucher 1954; Kaas et al. 1972; Peters and Feldman 1976; Caviness and Frost 1980; Simmons et al. 1982) and other mammals (Stone and Dreher 1973; Sanderson et al. 1980; Perkel et al. 1986; Kahn et al. 2000; Lyon 2007). In the present study, we also observed projections to V1 from LP, LD, PO, VL, AV, and VP. However, not all of these nuclei project to V1 in all cases (Fig. 13). For example, in case 07-76 in which the injection was in the expected location of the central visual field representation, connections with VL, VP, and PO were observed (Fig. 13A). In contrast in case 07-121, in which the injection was in the expected location of the peripheral visual field representation, connections with VL, VA, AV, and AM (anteromedial nucleus) were observed (Fig. 13B). LD and LP are associated with visual processing, PO and VP are associated with somatosensory processing, and VL is associated with motor processing.

Connections Reflect Behavioral Specializations

There are a number of examples of how connections reflect and likely subserve a variety of behaviors in different mammals and different sensory systems. For example, in primates, callosal connections between the hand representation in each hemisphere are conspicuously absent at early levels of processing in areas 3a, 3b, 1, and 2 but are extremely dense in posterior parietal cortical areas (e.g., Jones and Powell 1969; Killackey et al. 1983; Caminiti and Sbriccoli 1985; Shanks et al. 1985; Padberg et al. 2005; for review, see Krubitzer and Disbrow 2008). A number of studies using a variety of techniques (Hunter et al. 1976; Myers and Ebner 1976; Manzoni et al. 1984) indicate that these connections are necessary for intermanual transfer of information across hemispheres and coordination between the hands and between the hands and the eyes. In the visual system, callosal connections of V1 are predominantly with the vertical meridian representation of the opposite hemisphere, and such connections are proposed to establish functional continuity of visual hemifield representations for a unified percept of the visual field and to synchronize neuronal activity across hemispheres (e.g., Carmeli et al. 2007). Further, the connections of the central visual representations in V1 are different than of those of peripheral representations (e.g., Gattass et al. 2005; Ungerleider et al. 2008). For example, the central visual representation in V1 has projections to V4 (dorsomedial visual area), which peripheral representations do not. On the other hand, the peripheral visual representation in V1 has projections to V3A and PO. These connectional differences have been hypothesized to subserve different types of visual information processing such as object versus spatial vision (Gattass et al. 2005).

We observed specializations in cortical and thalamic connections of both V1 and A1 of the prairie vole that form the substrate for the multimodal neuronal responses that we observe in these fields. We postulate that this pattern of connectivity may be related to a constellation of behaviors associated with their unique niche. Interestingly, the multimodal connectivity of primary sensory areas that we observe in the present investigation has been observed in one other rodent, the Mongolian gerbil (Budinger et al. 2006). In contrast to most mammals and other rodents in particular, the prairie vole and the Mongolian gerbil are socially monogamous species (Agren 1984; Carter and Getz 1993). Prairie voles and Mongolian gerbils live in small groups, usually consisting of a pair-bonded male and female and 1–2 litters of young. They practice alloparenting in which the adult male and female as well as juveniles from the previous litter care for the young. The social interactions among prairie voles rely in part on early tactile experience, and differences in this early experience have dramatic effects on later social interactions (Bales et al. 2007). Finally, Mongolian gerbils and prairie vole pups have been observed to vocalize at much higher rates than mice and rats when separated from their mothers (Motomura et al. 2002), underscoring the important social role of auditory localization in these groups compared with other groups of rodents. Although Norway rats, house mice, and squirrels are social rodents, they are promiscuous. Males from promiscuous species do not generally live in the same burrow space as females nor do they form lifelong associations or pair bonds, and young are cared for exclusively by females (Poole 1985).

These behavioral differences in rodents have previously been linked with differences in hormones, gene expression, receptor type and distribution, forebrain connections, and cortical organization (Insel and Shapiro 1992; Carter 1998; Reep and Kirkpatrick 1999; Bales and Carter 2003; Lim et al. 2004; Campi et al. 2007). Therefore, it is reasonable to postulate that the differences in cortical and thalamic connections of auditory and visual cortex in the monogamous rodents versus the promiscuous rodents may be linked to communication that subserves important and unique social interactions. Budinger et al. (2006) propose that the multisensory integration that they observed in the Mongolian gerbil cortex may be important for sound source localization, spatial mnemonic functions, spatial attention and navigation, and even acquisition of conditioned fear response. Admittedly, the relationship between the extent of multisensory integration that occurs in the neocortex and the behaviors associated with particular social conditions within a group need to be further investigated, but the available data provide strong support for the proposition that complex social interactions that are mediated to a large extent by the auditory system require inputs from other sensory systems as well. Additional testing of these relationships would also include examination of other monogamous rodent species, such as the California mouse or Djungarian hamster.

Multisensory Processing Occurs at Multiple Stages of Processing

Integration of information from all sensory systems is necessary for establishing a unified representation of the environment necessary for making accurate decisions and responding appropriately to natural stimuli. However, where in the brain this integration takes place may be dependent on several factors including but not limited to the evolutionary history and derived developmental mechanisms of individual species, the size of the brain, the size of cortex and subcortical structures, the sensory environment, and specific demands that unique environments place on developing and evolving nervous systems. An interesting observation in prairie voles and Mongolian gerbils is that integration occurs in primary cortical sensory areas via corticocortical connections as well as thalamocortical connections. This seems somewhat surprising because multisensory integration in the cortex is generally thought to occur in higher order cortical areas such as in PPC and anterior ectosylvian sulcus (Wallace et al. 1992; Duhamel et al. 1998; Recanzone 2003; Cohen et al. 2005; Grefkes and Fink 2005; Stein and Stanford 2008). However, there are an accumulating number of studies that demonstrate that multisensory processing in primary fields may actually be more pervasive than previously believed. For example, studies in other mammals such as mice (Hunt et al. 2006), tenrecs (Krubitzer et al. 1997), rats (Wallace et al. 2004), and even monkeys (Brosch et al. 2005; Ghazanfar et al. 2005) have demonstrated multimodal responses in primary fields or at least modulation of neural response by different modalities of stimulation.

In terms of cortical connections, studies in cats demonstrate that A1 receives inputs from multisensory thalamic nuclei (e.g., Lee and Winer 2008a) and from multimodal cortical areas (e.g., Lee and Winer 2008b). Although A1 in monkeys appears to be interconnected only with other auditory cortical areas (e.g., Aitkin et al. 1988; de la Mothe et al. 2006; Hackett, Smiley, et al. 2007), it does receive thalamic inputs from multisensory nuclei (e.g., Hackett, de la Mothe, et al. 2007). For visual cortex, multisensory integration has been described between extrastriate cortical fields and the auditory core (e.g., Cappe and Barone 2005). Further, feedback connections to V1 originate in auditory cortex (e.g., Falchier et al. 2002; Clavagnier et al. 2004), but the source of these inputs was not localized to A1 (see Falchier et al. 2002). Feedback projections to V1 have also been demonstrated to be widespread and include projections from occipitotemporal and superior temporal regions, as well as parietal and auditory association areas (Rockland and Van Hoesen 1994; Rockland and Ojima 2003). Although it is clear that multisensory integration occurs much earlier in cortical processing hierarchies than was previously believed, direct connections between primary sensory areas or between second sensory areas and primary cortical areas of a different modality have not been observed in normal adult mammals, as in the present study or in Mongolian gerbils (Budinger et al. 2006). Further, inputs from primary thalamic projection nuclei associated with a particular sensory system (e.g., MG, LG, VP) to primary cortical fields that represent different modalities have not been observed in normal adult mammals.

In addition to the well-documented integration that occurs in the superior colliculus, multisensory integration has also been demonstrated at the level of the thalamus. For example, in rats, the dorsal and medial divisions of the MGN receive auditory input from the IC as well as somatosensory input from the spinal cord, and divisions of MG project directly to ventral auditory areas Te2 and Te3 (Ledoux et al. 1987; Donishi et al. 2006). Furthermore, electrophysiological recording of neurons in the MGm demonstrates multisensory responses to auditory and somatosensory stimuli in rats (Bordi and LeDoux 1994). In cats, auditory and somatosensory integration has been demonstrated in the MGm through connections from the external nucleus of the IC, and multimodal response properties have been observed in MGm, which in turn projects to A1 and AAF (Wepsic 1966; Calford and Aitkin 1983; Morel and Imig 1987).

Taken together, data from the present study in prairie voles as well as studies in other mammals indicate that integration of multisensory inputs occurs at early stages of cortical processing, and this early integration may be a general feature of organization shared by all mammals (Ghazanfar and Schroeder 2006). However, the precise pattern of connectivity that generates this integration is different for different mammals and, at the cortical level, appears to be associated with niche and the demands of a particular social environment.

Funding

McDonnell Grant to L.K., the National Institutes of Health (R01-NS35103 to L.K., MH0703022 to K.B. and S.C.); the National Eye Institute (T 32 EY015387); University of California; Davis Graduate Studies block; National Research Service Award (1F31NS062633-01A1 to K.C.).

We wish to thank Monica Chau, Sarah Karlen, DeLaine Larsen, and Adele Seelke for contributing to these experiments and for their helpful comments on this manuscript. Conflict of Interest: None declared.

References

Agren
A
Pair formation in the Mongolian gerbil
Anim Behav
 , 
1984
, vol. 
32
 (pg. 
528
-
535
)
Aitkin
LM
Kudo
M
Irvine
DRF
Connections of the primary auditory cortex in the common marmoset, Callithrix jacchus jacchus
J Comp Neurol
 , 
1988
, vol. 
269
 (pg. 
235
-
248
)
Allman
JM
Kaas
JH
Representation of the visual field in striate and adjoining cortex of the owl monkey (Aotus trivirgatus)
Brain Res
 , 
1971
, vol. 
35
 (pg. 
89
-
106
)
Allman
JM
Kaas
JH
The organization of the second visual area (V II) in the owl monkey: a second order transformation of the visual hemifield
Brain Res
 , 
1974
, vol. 
76
 (pg. 
247
-
265
)
Bales
KL
Carter
CS
Developmental exposure to oxytocin facilitates partner preferences in male prairie voles (Microtus ochrogaster)
Behav Neurosci
 , 
2003
, vol. 
117
 (pg. 
854
-
859
)
Bales
KL
Lewis-Reese
AD
Pfeifer
LA
Kramer
KM
Carter
CS
Early experience affects the traits of monogamy in a sexually dimorphic manner
Dev Psychobiol
 , 
2007
, vol. 
49
 (pg. 
335
-
342
)
Beck
PD
Pospichal
MW
Kaas
JH
Topography, architecture, and connections of somatosensory cortex in opossums: evidence for five somatosensory areas
J Comp Neurol
 , 
1996
, vol. 
366
 (pg. 
109
-
133
)
Benison
AM
Rector
DM
Barth
DS
Hemispheric mapping of secondary somatosensory cortex in the rat
J Neurophysiol
 , 
2007
, vol. 
97
 (pg. 
200
-
207
)
Blumberg
MS
Sokoloff
G
Do infant rats cry
Psychol Rev
 , 
2001
, vol. 
108
 (pg. 
83
-
95
)
Bordi
F
LeDoux
JE
Response properties of single units in areas of rat auditory thalamus that project to the amygdala. II. Cells receiving convergent auditory and somatosensory inputs and cells antidromically activated by amygdala stimulation
Exp Brain Res
 , 
1994
, vol. 
98
 (pg. 
275
-
286
)
Brosch
M
Selezneva
E
Scheich
H
Nonauditory events of a behavioral procedure activate auditory cortex of highly trained monkeys
J Neurosci
 , 
2005
, vol. 
25
 (pg. 
6797
-
6806
)
Budinger
E
Heil
P
Hess
A
Scheich
H
Multisensory processing via early cortical stages: connections of the primary auditory cortical field with other sensory systems
Neuroscience
 , 
2006
, vol. 
143
 (pg. 
1065
-
1083
)
Budinger
E
Laszcz
A
Lison
H
Scheich
H
Ohl
FW
Non-sensory cortical and subcortical connections of the primary auditory cortex in Mongolian gerbils: bottom-up and top-down processing of neuronal information via field AI
Brain Res
 , 
2008
, vol. 
1220
 (pg. 
2
-
32
)
Calford
MB
Aitkin
LM
Ascending projections to the medial geniculate body of the cat: evidence for multiple, parallel auditory pathways through thalamus
J Neurosci
 , 
1983
, vol. 
3
 (pg. 
2365
-
2380
)
Caminiti
R
Sbriccoli
A
The callosal system of the superior parietal lobule in the monkey
J Comp Neurol
 , 
1985
, vol. 
237
 (pg. 
85
-
99
)
Campi
KL
Karlen
SJ
Bales
KL
Krubitzer
L
Organization of sensory neocortex in prairie voles (Microtus ochrogaster)
J Comp Neurol
 , 
2007
, vol. 
502
 (pg. 
414
-
426
)
Cappe
C
Barone
P
Heteromodal connections supporting multisensory integration at low levels of cortical processing in the monkey
Eur J Neurosci
 , 
2005
, vol. 
22
 (pg. 
2886
-
2902
)
Carmeli
C
Lopez-Aguado
L
Schmidt
KE
De Feo
O
Innocenti
GM
A novel interhemispheric interaction: modulation of neuronal cooperativity in the visual areas
PLoS ONE
 , 
2007
, vol. 
2
 pg. 
e1287
 
Carroll
EW
Wong-Riley
MT
Quantitative light and electron microscopic analysis of cytochrome oxidase-rich zones in the striate cortex of the squirrel monkey
J Comp Neurol
 , 
1984
, vol. 
222
 (pg. 
1
-
17
)
Carter
CS
Neuroendocrine perspectives on social attachment and love
Psychoneuroendocrinology
 , 
1998
, vol. 
23
 (pg. 
779
-
818
)
Carter
CS
Getz
LL
Monogamy and the prairie vole
Sci Am
 , 
1993
, vol. 
268
 (pg. 
100
-
106
)
Catania
KC
Star-nosed moles
Curr Biol
 , 
2005
, vol. 
15
 (pg. 
R863
-
R864
)
Catania
KC
Jain
N
Franca
JG
Volchan
E
Kaas
JH
The organization of somatosensory cortex in the short-tailed opossum (Monodelphis domestica)
Somatosens Mot Res
 , 
2000
, vol. 
17
 (pg. 
39
-
51
)
Catania
KC
Kaas
JH
Organization of the somatosensory cortex of the star-nosed mole
J Comp Neurol
 , 
1995
, vol. 
351
 (pg. 
549
-
567
)
Catania
KC
Remple
FE
Tactile foveation in the star-nosed mole
Brain Behav Evol
 , 
2004
, vol. 
63
 (pg. 
1
-
12
)
Caviness
VS
Jr
Architectonic map of neocortex of the normal mouse
J Comp Neurol
 , 
1975
, vol. 
164
 (pg. 
247
-
263
)
Caviness
VS
Jr
Frost
DO
Tangential organization of thalamic projections to the neocortex in the mouse
J Comp Neurol
 , 
1980
, vol. 
194
 (pg. 
335
-
367
)
Chapin
JK
Lin
CS
Mapping the body representation in the SI cortex of anesthetized and awake rats
J Comp Neurol
 , 
1984
, vol. 
229
 (pg. 
199
-
213
)
Clavagnier
S
Falchier
A
Kennedy
H
Long-distance feedback projections to area V1: implications for multisensory integration, spatial awareness, and visual consciousness
Cogn Affect Behav Neurosci
 , 
2004
, vol. 
4
 (pg. 
117
-
126
)
Cohen
YE
Russ
BE
Gifford
GW
3rd
Auditory processing in the posterior parietal cortex
Behav Cogn Neurosci Rev
 , 
2005
, vol. 
4
 (pg. 
218
-
231
)
Coogan
TA
Burkhalter
A
Hierarchical organization of areas in rat visual cortex
J Neurosci
 , 
1993
, vol. 
13
 (pg. 
3749
-
3772
)
de la Mothe
LA
Blumell
S
Kajikawa
Y
Hackett
TA
Thalamic connections of the auditory cortex in marmoset monkeys: core and medial belt regions
J Comp Neurol
 , 
2006
, vol. 
496
 (pg. 
72
-
96
)
Donishi
T
Kimura
A
Okamoto
K
Tamai
Y
“Ventral” area in the rat auditory cortex: a major auditory field connected with the dorsal division of the medial geniculate body
Neuroscience
 , 
2006
, vol. 
141
 (pg. 
1553
-
1567
)
Duhamel
JR
Colby
CL
Goldberg
ME
Ventral intraparietal area of the macaque: congruent visual and somatic response properties
J Neurophysiol
 , 
1998
, vol. 
79
 (pg. 
126
-
136
)
Ehret
G
Infant rodent ultrasounds—a gate to the understanding of sound communication
Behav Genet
 , 
2005
, vol. 
33
 (pg. 
19
-
29
)
Falchier
A
Clavagnier
S
Barone
P
Kennedy
H
Anatomical evidence of multimodal integration in primate striate cortex
J Neurosci
 , 
2002
, vol. 
22
 (pg. 
5749
-
5759
)
Fang
PC
Stepniewska
I
Kaas
JH
Ipsilateral cortical connections of motor, premotor, frontal eye, and posterior parietal fields in a prosimian primate, Otolemur garnetti
J Comp Neurol
 , 
2005
, vol. 
490
 (pg. 
305
-
333
)
Felleman
DJ
Nelson
RJ
Sur
M
Kaas
JH
Representations of the body surface in areas 3b and 1 of postcentral parietal cortex of Cebus monkeys
Brain Res
 , 
1983
, vol. 
268
 (pg. 
15
-
26
)
Gallyas
F
Silver staining of myelin by means of physical development
Neurol Res
 , 
1979
, vol. 
1
 (pg. 
203
-
209
)
Gattass
R
Nascimento-Silva
S
Soares
JG
Lima
B
Jansen
AK
Diogo
AC
Farias
MF
Botelho
MM
Mariani
OS
Azzi
J
, et al.  . 
Cortical visual areas in monkeys: location, topography, connections, columns, plasticity and cortical dynamics
Philos Trans R Soc Lond B Biol Sci
 , 
2005
, vol. 
360
 (pg. 
709
-
731
)
Ghazanfar
AA
Maier
JX
Hoffman
KL
Logothetis
NK
Multisensory integration of dynamic faces and voices in rhesus monkey auditory cortex
J Neurosci
 , 
2005
, vol. 
25
 (pg. 
5004
-
5012
)
Ghazanfar
AA
Schroeder
CE
Is neocortex essentially multisensory?
Trends Cogn Sci
 , 
2006
, vol. 
10
 (pg. 
278
-
285
)
Grefkes
C
Fink
GR
The functional organization of the intraparietal sulcus in humans and monkeys
J Anat
 , 
2005
, vol. 
207
 (pg. 
3
-
17
)
Hackett
TA
de la Mothe
LA
Ulbert
I
Karmos
G
Smiley
J
Schroeder
CE
Multisensory convergence in auditory cortex, II. Thalamocortical connections of the caudal superior temporal plane
J Comp Neurol
 , 
2007
, vol. 
502
 (pg. 
924
-
952
)
Hackett
TA
Smiley
JF
Ulbert
I
Karmos
G
Lakatos
P
de la Mothe
LA
Schroeder
CE
Sources of somatosensory input to the caudal belt areas of auditory cortex
Perception
 , 
2007
, vol. 
36
 (pg. 
1419
-
1430
)
Hall
WC
Kaas
JH
Killackey
H
Diamond
IT
Cortical visual areas in the grey squirrel (Sciurus carolinensis): a correlation between cortical evoked potential maps and architectonic subdivisions
J Neurophysiol
 , 
1971
, vol. 
34
 (pg. 
437
-
452
)
Hubel
DH
Wiesel
TN
Receptive fields and functional architecture of monkey striate cortex
J Physiol
 , 
1968
, vol. 
195
 (pg. 
215
-
243
)
Hunt
DL
Yamoah
EN
Krubitzer
L
Multisensory plasticity in congenitally deaf mice: how are cortical areas functionally specified?
Neuroscience
 , 
2006
, vol. 
139
 (pg. 
1507
-
1524
)
Hunter
M
Maccabe
JJ
Ettlinger
G
Intermanual transfer of tactile training in the monkey: the effect of bilateral parieto-prestriate ablations
Neuropsychologia
 , 
1976
, vol. 
14
 (pg. 
385
-
389
)
Insel
TR
Shapiro
LE
Oxytocin receptor distribution reflects social organization in monogamous and polygamous voles
Proc Natl Acad Sci USA
 , 
1992
, vol. 
89
 (pg. 
5981
-
5985
)
Jain
N
Catania
KC
Kaas
JH
A histologically visible representation of the fingers and palm in primate area 3b and its immutability following long-term deafferentations
Cereb Cortex
 , 
1998
, vol. 
8
 (pg. 
227
-
236
)
Jones
EG
Powell
TP
Connexions of the somatic sensory cortex of the rhesus monkey. II. Contralateral cortical connexions
Brain
 , 
1969
, vol. 
92
 (pg. 
717
-
730
)
Kaas
JH
What, if anything, is SI? Organization of first somatosensory area of cortex
Physiol Rev
 , 
1983
, vol. 
63
 (pg. 
206
-
231
)
Kaas
JH
Hall
WC
Diamond
IT
Visual cortex of the grey squirrel (Sciurus carolinensis): architectonic subdivisions and connections from the visual thalamus
J Comp Neurol
 , 
1972
, vol. 
145
 (pg. 
273
-
305
)
Kaas
JH
Krubitzer
LA
Chino
YM
Langston
AL
Polley
EH
Blair
N
Reorganization of retinotopic cortical maps in adult mammals after lesions of the retina
Science
 , 
1990
, vol. 
248
 (pg. 
229
-
231
)
Kaas
JH
Krubitzer
LA
Johanson
KL
Cortical connections of areas 17 (V-I) and 18 (V-II) of squirrels
J Comp Neurol
 , 
1989
, vol. 
281
 (pg. 
426
-
446
)
Kahn
DM
Huffman
KJ
Krubitzer
L
Organization and connections of V1 in Monodelphis domestica
J Comp Neurol
 , 
2000
, vol. 
428
 (pg. 
337
-
354
)
Kalatsky
VA
Polley
DB
Merzenich
MM
Schreiner
CE
Stryker
MP
Fine functional organization of auditory cortex revealed by Fourier optical imaging
Proc Natl Acad Sci USA
 , 
2005
, vol. 
102
 (pg. 
13325
-
13330
)
Karlen
SJ
Krubitzer
L
The functional and anatomical organization of marsupial neocortex: evidence for parallel evolution across mammals
Prog Neurobiol
 , 
2007
, vol. 
82
 (pg. 
122
-
141
)
Killackey
HP
Gould
HJ
3rd
Cusick
CG
Pons
TP
Kaas
JH
The relation of corpus callosum connections to architectonic fields and body surface maps in sensorimotor cortex of new and old world monkeys
J Comp Neurol
 , 
1983
, vol. 
219
 (pg. 
384
-
419
)
Kimura
A
Donishi
T
Okamoto
K
Imbe
H
Tamai
Y
Efferent connections of the ventral auditory area in the rat cortex: implications for auditory processing related to emotion
Eur J Neurosci
 , 
2007
, vol. 
25
 (pg. 
2819
-
2834
)
Kimura
A
Donishi
T
Sakoda
T
Hazama
M
Tamai
Y
Auditory thalamic nuclei projections to the temporal cortex in the rat
Neuroscience
 , 
2003
, vol. 
117
 (pg. 
1003
-
1016
)
Krubitzer
L
Campi
K
Squire
LR
Neocortical organization in monotremes
Encyclopedia of neuroscience
 , 
2009
Oxford (UK)
Academic Press
(pg. 
51
-
59
)
Krubitzer
L
Clarey
J
Tweedale
R
Elston
G
Calford
M
A redefinition of somatosensory areas in the lateral sulcus of macaque monkeys
J Neurosci
 , 
1995
, vol. 
15
 (pg. 
3821
-
3839
)
Krubitzer
L
Disbrow
E
Kaas
J
Gardner
E
The evolution of parietal areas involved in hand use in primates
The senses: a comprehensive reference
 , 
2008
London
Elsevier
(pg. 
183
-
214
)
Krubitzer
L
Kunzle
H
Kaas
J
Organization of sensory cortex in a Madagascan insectivore, the tenrec (Echinops telfairi)
J Comp Neurol
 , 
1997
, vol. 
379
 (pg. 
399
-
414
)
Krubitzer
L
Manger
P
Pettigrew
J
Calford
M
Organization of somatosensory cortex in monotremes: in search of the prototypical plan
J Comp Neurol
 , 
1995
, vol. 
351
 (pg. 
261
-
306
)
Krubitzer
LA
Calford
MB
Five topographically organized fields in the somatosensory cortex of the flying fox: microelectrode maps, myeloarchitecture, and cortical modules
J Comp Neurol
 , 
1992
, vol. 
317
 (pg. 
1
-
30
)
Krubitzer
LA
Sesma
MA
Kaas
JH
Microelectrode maps, myeloarchitecture, and cortical connections of three somatotopically organized representations of the body surface in the parietal cortex of squirrels
J Comp Neurol
 , 
1986
, vol. 
250
 (pg. 
403
-
430
)
Lee
CC
Winer
JA
Connections of cat auditory cortex: I. Thalamocortical system
J Comp Neurol
 , 
2008
, vol. 
507
 (pg. 
1879
-
1900
)
Lee
CC
Winer
JA
Connections of cat auditory cortex: III. Corticocortical system
J Comp Neurol
 , 
2008
, vol. 
507
 (pg. 
1920
-
1943
)
Ledoux
JE
Ruggiero
DA
Forest
R
Stornetta
R
Reis
DJ
Topographic organization of convergent projections to the thalamus from the inferior colliculus and spinal cord in the rat
J Comp Neurol
 , 
1987
, vol. 
264
 (pg. 
123
-
146
)
Lepri
JJ
Theodorides
M
Wysocki
CJ
Ultrasonic vocalizations by adult prairie voles, Microtus ochrogaster
Experientia
 , 
1988
, vol. 
44
 (pg. 
271
-
273
)
Lim
MM
Hammock
EA
Young
LJ
The role of vasopressin in the genetic and neural regulation of monogamy
J Neuroendocrinol
 , 
2004
, vol. 
16
 (pg. 
325
-
332
)
Llano
DA
Sherman
SM
Evidence for nonreciprocal organization of the mouse auditory thalamocortical-corticothalamic projection systems
J Comp Neurol
 , 
2008
, vol. 
507
 (pg. 
1209
-
1227
)
Luethke
LE
Krubitzer
LA
Kaas
JH
Cortical connections of electrophysiologically and architectonically defined subdivisions of auditory cortex in squirrels
J Comp Neurol
 , 
1988
, vol. 
268
 (pg. 
181
-
203
)
Lyon
D
Kaas
J
Krubitzer
L
The evolution of visual cortex and visual systems
Evolution of nervous systems: a comprehensive reference
 , 
2007
Oxford
Elsevier
(pg. 
267
-
306
)
Malach
R
Patterns of connections in rat visual cortex
J Neurosci
 , 
1989
, vol. 
9
 (pg. 
3741
-
3752
)
Manzoni
T
Barbaresi
P
Conti
F
Callosal mechanism for the interhemispheric transfer of hand somatosensory information in the monkey
Behav Brain Res
 , 
1984
, vol. 
11
 (pg. 
155
-
170
)
Merzenich
MM
Kaas
JH
Roth
GL
Auditory cortex in the grey squirrel: tonotopic organization and architectonic fields
J Comp Neurol
 , 
1976
, vol. 
166
 (pg. 
387
-
401
)
Miller
MW
Vogt
BA
Direct connections of rat visual cortex with sensory, motor, and association cortices
J Comp Neurol
 , 
1984
, vol. 
226
 (pg. 
184
-
202
)
Montero
VM
Evoked responses in the rat's visual cortex to contralateral, ipsilateral and restricted photic stimulation
Brain Res
 , 
1973
, vol. 
53
 (pg. 
192
-
196
)
Montero
VM
Retinotopy of cortical connections between the striate cortex and extrastriate visual areas in the rat
Exp Brain Res
 , 
1993
, vol. 
94
 (pg. 
1
-
15
)
Morel
A
Imig
TJ
Thalamic projections to fields A, AI, P, and VP in the cat auditory cortex
J Comp Neurol
 , 
1987
, vol. 
265
 (pg. 
119
-
144
)
Motomura
N
Shimizu
K
Shimizu
M
Aoki-Komori
S
Taniguchi
K
Serizawa
I
Saito
TR
A comparative study of isolation-induced ultrasonic vocalization in rodent pups
Exp Anim
 , 
2002
, vol. 
51
 (pg. 
187
-
190
)
Mountcastle
VB
The sensory hand: neural mechanisms of somatic sensation
 , 
2005
Boston (MA)
Harvard University Press
Myers
RE
Ebner
FF
Localization of function in corpus callosum: tactual information transmission in Macaca mulatta
Brain Res
 , 
1976
, vol. 
103
 (pg. 
455
-
462
)
Naatanen
R
Lehtokoski
A
Lennes
M
Cheour
M
Huotilainen
M
Iivonen
A
Vainio
M
Alku
P
Ilmoniemi
RJ
Luuk
A
, et al.  . 
Language-specific phoneme representations revealed by electric and magnetic brain responses
Nature
 , 
1997
, vol. 
385
 (pg. 
432
-
434
)
Nauta
WJ
Bucher
VM
Efferent connections of the striate cortex in the albino rat
J Comp Neurol
 , 
1954
, vol. 
100
 (pg. 
257
-
285
)
Nelson
RJ
Sur
M
Felleman
DJ
Kaas
JH
Representations of the body surface in postcentral parietal cortex of Macaca fascicularis
J Comp Neurol
 , 
1980
, vol. 
192
 (pg. 
611
-
643
)
Neville
HJ
Bavelier
D
Corina
D
Rauschecker
J
Karni
A
Lalwani
A
Braun
A
Clark
V
Jezzard
P
Turner
R
Cerebral organization for language in deaf and hearing subjects: biological constraints and effects of experience
Proc Natl Acad Sci USA
 , 
1998
, vol. 
95
 (pg. 
922
-
929
)
Nussbaumer
JC
Van der Loos
H
An electrophysiological and anatomical study of projections to the mouse cortical barrelfield and its surroundings
J Neurophysiol
 , 
1985
, vol. 
53
 (pg. 
686
-
698
)
Olavarria
J
Montero
V
Elaborate organization of visual cortex in the hamster
Neurosci Res
 , 
1990
, vol. 
8
 (pg. 
40
-
47
)
Padberg
J
Disbrow
E
Krubitzer
L
The organization and connections of anterior and posterior parietal cortex in titi monkeys: do New World monkeys have an area 2?
Cereb Cortex
 , 
2005
, vol. 
15
 (pg. 
1938
-
1963
)
Padberg
J
Franca
JG
Cooke
DF
Soares
JG
Rosa
MG
Fiorani
M
Jr
Gattass
R
Krubitzer
L
Parallel evolution of cortical areas involved in skilled hand use
J Neurosci
 , 
2007
, vol. 
27
 (pg. 
10106
-
10115
)
Payne
BR
Evidence for visual cortical area homologs in cat and macaque monkey
Cereb Cortex
 , 
1993
, vol. 
3
 (pg. 
1
-
25
)
Penfield
W
Rasmussen
T
The cerebral cortex of man: a clinical study of localization of function
 , 
1968
New York
Hafner publishing
Perkel
DJ
Bullier
J
Kennedy
H
Topography of the afferent connectivity of area 17 in the macaque monkey: a double-labelling study
J Comp Neurol
 , 
1986
, vol. 
253
 (pg. 
374
-
402
)
Peters
A
Feldman
ML
The projection of the lateral geniculate nucleus to area 17 of the rat cerebral cortex. I. General description
J Neurocytol
 , 
1976
, vol. 
5
 (pg. 
63
-
84
)
Pettigrew
JD
Manger
PR
Fine
SL
The sensory world of the platypus
Philos Trans R Soc Lond B Biol Sci
 , 
1998
, vol. 
353
 (pg. 
1199
-
1210
)
Polley
DB
Read
HL
Storace
DA
Merzenich
MM
Multiparametric auditory receptive field organization across five cortical fields in the albino rat
J Neurophysiol
 , 
2007
, vol. 
97
 (pg. 
3621
-
3638
)
Poole
T
Social behavior in mammals
 , 
1985
New York
Blackie & Sons Ltd
Portfors
CV
Types of functions of ultrasonic vocalizations in laboratory rats and mice
J Am Assoc Lab Anim Sci
 , 
2007
, vol. 
46
 (pg. 
28
-
34
)
Pubols
BH
Jr
Pubols
LM
Somatotopic organization of spider monkey somatic sensory cerebral cortex
J Comp Neurol
 , 
1971
, vol. 
141
 (pg. 
63
-
75
)
Rabon
D
Sawrey
D
Webster
W
Infant ultrasonic vocalizations and parental responses in two species of voles (Microtus)
Canadian Journal of Zoology
 , 
2001
, vol. 
79
 (pg. 
830
-
837
)
Recanzone
GH
Auditory influences on visual temporal rate perception
J Neurophysiol
 , 
2003
, vol. 
89
 (pg. 
1078
-
1093
)
Reep
RL
Kirkpatrick
B
Forebrain connections of medial agranular cortex in the prairie vole, Microtus ochrogaster
Exp Brain Res
 , 
1999
, vol. 
126
 (pg. 
336
-
350
)
Remple
MS
Henry
EC
Catania
KC
Organization of somatosensory cortex in the laboratory rat (Rattus norvegicus): evidence for two lateral areas joined at the representation of the teeth
J Comp Neurol
 , 
2003
, vol. 
467
 (pg. 
105
-
118
)
Rockland
KS
Ojima
H
Multisensory convergence in calcarine visual areas in macaque monkey
Int J Psychophysiol
 , 
2003
, vol. 
50
 (pg. 
19
-
26
)
Rockland
KS
Van Hoesen
GW
Direct temporal-occipital feedback connections to striate cortex (V1) in the macaque monkey
Cereb Cortex
 , 
1994
, vol. 
4
 (pg. 
300
-
313
)
Roger
M
Arnault
P
Anatomical study of the connections of the primary auditory area in the rat
J Comp Neurol
 , 
1989
, vol. 
287
 (pg. 
339
-
356
)
Rosa
MG
Krubitzer
LA
Molnar
Z
Nelson
JE
Organization of visual cortex in the northern quoll, Dasyurus hallucatus: evidence for a homologue of the second visual area in marsupials
Eur J Neurosci
 , 
1999
, vol. 
11
 (pg. 
907
-
915
)
Rutkowski
RG
Miasnikov
AA
Weinberger
NM
Characterisation of multiple physiological fields within the anatomical core of rat auditory cortex
Hear Res
 , 
2003
, vol. 
181
 (pg. 
116
-
130
)
Sanderson
KJ
Haight
JR
Pearson
LJ
Transneuronal transport of tritiated fucose and proline in the visual pathways of the brushtailed possum, Trichosurus vulpecula
Neurosci Lett
 , 
1980
, vol. 
20
 (pg. 
243
-
248
)
Shanks
MF
Pearson
RC
Powell
TP
The callosal connexions of the primary somatic sensory cortex in the monkey
Brain Res
 , 
1985
, vol. 
356
 (pg. 
43
-
65
)
Shapiro
LE
Insel
TR
Infant's response to social separation reflects adult differences in affiliative behavior: a comparative developmental study in prairie and montane voles
Dev Psychobiol
 , 
1990
, vol. 
23
 (pg. 
375
-
393
)
Shi
CJ
Cassell
MD
Cortical, thalamic, and amygdaloid projections of rat temporal cortex
J Comp Neurol
 , 
1997
, vol. 
382
 (pg. 
153
-
175
)
Simmons
PA
Lemmon
V
Pearlman
AL
Afferent and efferent connections of the striate and extrastriate visual cortex of the normal and reeler mouse
J Comp Neurol
 , 
1982
, vol. 
211
 (pg. 
295
-
308
)
Stein
BE
Stanford
TR
Multisensory integration: current issues from the perspective of the single neuron
Nat Rev Neurosci
 , 
2008
, vol. 
9
 (pg. 
255
-
266
)
Stiebler
I
Neulist
R
Fichtel
I
Ehret
G
The auditory cortex of the house mouse: left-right differences, tonotopic organization and quantitative analysis of frequency representation
J Comp Physiol [A]
 , 
1997
, vol. 
181
 (pg. 
559
-
571
)
Stone
J
Dreher
B
Projection of X- and Y-cells of the cat's lateral geniculate nucleus to areas 17 and 18 of visual cortex
J Neurophysiol
 , 
1973
, vol. 
36
 (pg. 
551
-
567
)
Suga
N
Niwa
H
Taniguchi
I
Margoliash
D
The personalized auditory cortex of the mustached bat: adaptation for echolocation
J Neurophysiol
 , 
1987
, vol. 
58
 (pg. 
643
-
654
)
Sur
M
Nelson
RJ
Kaas
JH
The representation of the body surface in somatosensory area I of the grey squirrel
J Comp Neurol
 , 
1978
, vol. 
179
 (pg. 
425
-
449
)
Suta
D
Popelar
J
Syka
J
Coding of communication calls in the subcortical and cortical structures of the auditory system
Physiol Res
 , 
2008
, vol. 
57
 
3 Suppl
(pg. 
S149
-
S159
)
Thomas
H
Lopez
V
Comparative study of inter- and intrahemispheric cortico-cortical connections in gerbil auditory cortex
Biol Res
 , 
2003
, vol. 
36
 (pg. 
155
-
169
)
Thomas
H
Tillein
J
Heil
P
Scheich
H
Functional organization of auditory cortex in the mongolian gerbil (Meriones unguiculatus). I. Electrophysiological mapping of frequency representation and distinction of fields
Eur J Neurosci
 , 
1993
, vol. 
5
 (pg. 
882
-
897
)
Thomas
HC
Espinoza
SG
Relationships between interhemispheric cortical connections and visual areas in hooded rats
Brain Res
 , 
1987
, vol. 
417
 (pg. 
214
-
224
)
Tiao
YC
Blakemore
C
Functional organization in the visual cortex of the golden hamster
J Comp Neurol
 , 
1976
, vol. 
168
 (pg. 
459
-
481
)
Tusa
RJ
Rosenquist
AC
Palmer
LA
Retinotopic organization of areas 18 and 19 in the cat
J Comp Neurol
 , 
1979
, vol. 
185
 (pg. 
657
-
678
)
Ungerleider
LG
Galkin
TW
Desimone
R
Gattass
R
Cortical connections of area V4 in the macaque
Cereb Cortex
 , 
2008
, vol. 
18
 (pg. 
477
-
499
)
Veenman
CL
Reiner
A
Honig
MG
Biotinylated dextran amine as an anterograde tracer for single- and double-labeling studies
J Neurosci Methods
 , 
1992
, vol. 
41
 (pg. 
239
-
254
)
Vidyasagar
TR
Wye-Dvorak
J
Henry
GH
Mark
RF
Cytoarchitecture and visual field representation in area 17 of the tammar wallaby (Macropus eugenii)
J Comp Neurol
 , 
1992
, vol. 
325
 (pg. 
291
-
300
)
Wagor
E
Mangini
NJ
Pearlman
AL
Retinotopic organization of striate and extrastriate visual cortex in the mouse
J Comp Neurol
 , 
1980
, vol. 
193
 (pg. 
187
-
202
)
Wallace
MT
Meredith
MA
Stein
BE
Integration of multiple sensory modalities in cat cortex
Exp Brain Res
 , 
1992
, vol. 
91
 (pg. 
484
-
488
)
Wallace
MT
Ramachandran
R
Stein
BE
A revised view of sensory cortical parcellation
Proc Natl Acad Sci USA
 , 
2004
, vol. 
101
 (pg. 
2167
-
2172
)
Wang
Q
Burkhalter
A
Area map of mouse visual cortex
J Comp Neurol
 , 
2007
, vol. 
502
 (pg. 
339
-
357
)
Welker
C
Microelectrode delineation of fine grain somatotopic organization of (SmI) cerebral neocortex in albino rat
Brain Res
 , 
1971
, vol. 
26
 (pg. 
259
-
275
)
Wepsic
JG
Multimodal sensory activation of cells in the magnocellular medial geniculate nucleus
Exp Neurol
 , 
1966
, vol. 
15
 (pg. 
299
-
318
)
Wiesendanger
M
Manual dexterity and the making of tools—an introduction from an evolutionary perspective
Exp Brain Res
 , 
1999
, vol. 
128
 (pg. 
1
-
5
)
Winer
JA
Sally
SL
Larue
DT
Kelly
JB
Origins of medial geniculate body projections to physiologically defined zones of rat primary auditory cortex
Hear Res
 , 
1999
, vol. 
130
 (pg. 
42
-
61
)
Wing
A
Haggard
P
Flanagan
J
Hand and brain: neurophysiology and psychology of hand movements
 , 
1996
London
Academic Press
Wong
P
Gharbawie
OA
Luethke
LE
Kaas
JH
Thalamic connections of architectonic subdivisions of temporal cortex in grey squirrels (Sciurus carolinensis)
J Comp Neurol
 , 
2008
, vol. 
510
 (pg. 
440
-
461
)
Wong
P
Kaas
JH
Architectonic subdivisions of neocortex in the gray squirrel (Sciurus carolinensis)
Anat Rec (Hoboken)
 , 
2008
, vol. 
291
 (pg. 
1301
-
1333
)
Woolsey
TA
Somatosensory, auditory and visual cortical areas of the mouse
Johns Hopkins Med J
 , 
1967
, vol. 
121
 (pg. 
91
-
112
)