The Significance of Background Odour for an Egg Parasitoid to Detect Plants with Host Eggs

Abstract

Scots pine has been shown to produce a volatile bouquet that attracts egg parasitoids in response to oviposition of the herbivorous sawfly Diprion pini. Previous analyses of headspace volatiles of oviposition-induced pine twigs revealed only quantitative changes; in particular, the sesquiterpene (E)-β-farnesene was emitted in significantly higher quantities by oviposition-induced pine. Here we investigated whether (E)-β-farnesene attracted the egg parasitoid Chrysonotomyia ruforum. We tested the behavioural response of C. ruforum females to different concentrations of (E)-β-farnesene. Egg parasitoids did not respond to this sesquiterpene at either concentration tested. However, they did respond significantly to (E)-β-farnesene when this compound was offered in combination with the volatile blend emitted from pine twigs without eggs. This response was dependent on the applied concentration of (E)-β-farnesene. Further bioassays with other components [(E)-β-caryophyllene, δ-cadinene] of the odour blend of pine were conducted in combination with the volatile blend from egg-free pine as background odour. None of the compounds tested against the background of odour from an egg-free pine twig were attractive to the egg parasitoid. These results suggest that the egg parasitoids responded specifically to (E)-β-farnesene, but only when this compound was experienced in the ‘right’ context, i.e. when contrasted with a background odour of non-oviposition-induced pine volatiles.

Diprion pini, egg deposition, (E)-β-farnesene, induced defense, Pinus sylvestris, sesquiterpenes