P5329
Gene expression of sirtuin-1 and receptor for advanced glycation end products in healthy and slightly overweight subjects after caloric restriction and resveratrol administration

Extract

Background: Sirtuin-1 (Sirt1) and soluble receptor for advanced glycation end product (sRAGE) were associated with vascular protection. Sirt1 plays an important role in vascular biology, and regulates aspects of age-dependent atherosclerosis. sRAGE are serum receptors scavengers for advanced glycation end products (AGE). They reduce AGE availability for RAGE receptor located in the cell membrane and therefore decrease harmful effects on cells. In animals, both systems are influenced by resveratrol (RSV) and caloric restriction (CR).

Purpose: To examine the effects of RSV and CR on gene expression of Sirt1 and RAGE and on serum levels of Sirt1 and sRAGE in healthy and slightly overweight subjects.

Methods: Study randomized 48 healthy subjects (24 women), aged 55 to 65, to 30 days of RSV (500 mg/day) or CR (1000cal/d). Blood were collected at baseline and at 30 days. Laboratory data analyzed were: triglycerides, total cholesterol (TC), HDL, LDL, non-HDL cholesterol (non-HDL), apolipoprotein A1 (apoA1) and B (apoB), lipoprotein (a), glucose, C-reactive protein (CRP), HOMA-IR, Sirt1 and sRAGE. Gene expression of Sirt1 and RAGE were analyzed by real-time polymerase chain reaction.