P3195
Impaired functioning and low mass of skeletal muscles in men with heart failure with reduced ejection fraction are reflected by reduced muscle-derived irisin

Extract

Background: Energy metabolism in adipose, bone and muscle tissues is integrated, among others, by irisin. This circulating hormone-like myokine at adequately high level secures optimal exercise capacity and metabolic homeostasis.

Purpose: The aim of the study was to assess the production of irisin in the blood draining exercising forearm muscles and in peripheral blood in relation to the mass and functioning of skeletal muscles in men with heart failure and healthy men.

Methods: We studied population comprised of 53 men with stable heart failure with reduced ejection fraction (HFrEF) (LVEF ≤40%); mean age: ±64 years; NYHA class I-II: 87%) and 15 middle-aged healthy men. Lean mass of upper extremities were determined by DEXA (dual-energy X-ray absorptiometry). We analysed the levels of irisin in plasma samples from peripheral blood from all patients by ELISA. Further, we analyzed samples taken from antecubital veins which drain the forearm muscle before and after local physical exercise (standardized 5-minute handgrip exercise) for the aforementioned myokine.