Abstract

Aims

Although inflammation contributes to cardiovascular disease, the associations of appendectomy and tonsillectomy, which remove mucosa-associated lymphoid tissue, with risk of acute myocardial infarction (AMI) are unknown. Our aim was to assess the association between these operations performed in childhood and AMI risk later in life.

Methods and results

We conducted a prospective matched cohort study among all Swedish residents born between 1955 and 1970. A national register identified all appendectomies and tonsillectomies. For each patient undergoing appendectomy or tonsillectomy, we randomly selected five controls without the history of the respective operation, matched on sex, age, and county of residence. Participants were followed for fatal and non-fatal AMI for an average of 23.5 years. Because appendiceal and tonsillar tissues have reduced function after adolescence, our primary analyses were restricted to individuals below age 20 at the time of surgery (54 449 appendectomies and 27 284 tonsillectomies). We derived hazard ratios (HRs) from proportional hazard models adjusted for parental occupation and parental history of AMI. Operations before 20 years of age were associated with an increased risk for AMI (417 and 216 events in the appendectomy and tonsillectomy datasets, respectively), with adjusted HRs of 1.33 [95% confidence interval (CI), 1.05–1.70] for appendectomy and 1.44 (95% CI, 1.04–2.01) for tonsillectomy. This association was graded, with the highest risk among those undergoing both procedures, and generally similar among both males and females. Appendectomy and tonsillectomy performed at or above 20 years of age were not associated with the risk of AMI.

Conclusions

We found a higher risk of AMI related to surgical removal of the tonsils and appendix before age 20. These results are consistent with the hypothesis that subtle alterations in immune function following these operations may alter the subsequent cardiovascular risk, but further studies are needed to confirm these findings and to explore possible mechanisms.

Introduction

The cecal appendix and tonsils are secondary lymphoid organs and prominent constituents of the mucosa-associated lymphoid tissue (MALT) system. The lymphoid function of these tissues is particularly pronounced at younger ages.1–5

Operative procedures to remove the appendix or tonsils are among the most common surgeries, particularly in children and in young adults,1,6 with a lifetime risk of appendectomy estimated to be 10–20%7,8 and a risk nearly as high for tonsillectomy just through to age 20.9 Concordant with the role of these tissues in immune function in children and adolescents, the long-term health effects of the removal of these secondary lymphoid organs seem to be restricted to operations that occur before adulthood.10–13 Long-term health effects attributed to such procedures include moderate changes in immune function,4,14 elevated risk for Hodgkin's lymphoma,10,15 and alteration in risk for some autoimmune disorders.11–13,16–19

Given the strong biological and epidemiological evidence linking inflammation with coronary heart disease (CHD),20 one might anticipate that surgical MALT removal, with its consequent effects on immunity, might also have a long-term effect on CHD. However, we are aware of no studies that have evaluated the potential effects of appendectomy or tonsillectomy on atherosclerosis or CHD risk. To address this possibility, we used Sweden's unique health and administrative registers to investigate whether appendectomy or tonsillectomy, particularly in childhood or adolescence, is associated with acute myocardial infarction (AMI).

Methods

Study population

The study was based on data from the TWELVE-Register, which incorporates 12 of Sweden's national health and administrative registers.21,22 All persons born between 1955 and 1985 residing in Sweden at any time until 1 January 2003 are included in the TWELVE-Register. To ensure adequate follow-up among the young individuals included in this study, we restricted our analyses to those born between 1955 and 1970.

Appendectomy and tonsillectomy

Appendectomies (operation codes JEA00, JEA01, JEA10, 4510, 4511) and tonsillectomies (operation codes EMB10, EMB20, EMB30, EMB99, 2710, 2720) were identified with a link to the Swedish Inpatient Register, which started regionally in 1964 and since 1987 provides virtually complete information on all inpatient care and corresponding diagnoses in Sweden.

We categorized the underlying diagnoses for appendectomy as follows: (i) perforated or abscessed appendicitis; (ii) acute appendicitis without perforation or abscess; and (iii) appendectomy without underlying appendicitis.

Our primary analyses were restricted to individuals below age 20 at the time of operation, the approximate age at which MALT tissue would be anticipated to become less physiologically significant and used by other studies on long-term health effects of appendectomy.1,2,11,12 For comparison, we also examined risk in the smaller subset of individuals who underwent these surgeries at age 20 or older and those at below 15 years of age.

Unexposed controls

The present study had a matched cohort design. For each patient with an appendectomy or tonsillectomy, we randomly selected five matched controls without history of the respective operation. The matching factors were sex, birth year (in yearly increments), and county of residence at the time of surgery. To identify county of residence at the time of surgery, we used the data from the closest nationwide Population and Housing Census. Censuses were conducted every five years from 1960 until 1990. Participants with missing census data were not included. Unexposed controls who died or emigrated between the census and the operation were excluded.

Outcome ascertainment

Participants were followed with linkage to the respective health or administrative registers from the date of the index operation or the corresponding time for the unexposed controls until fatal or non-fatal AMI, death owing to other causes, emigration from Sweden, or the end of follow-up, i.e. 31 December 2002. Detection method of AMI was identical to that of the national Swedish Myocardial Infarction Register. It was based on record linkage between the Swedish Inpatient Register and the Cause of Death Register using ICD9 code 410 and ICD10 codes I21 and I22 for reasons of hospitalization or underlying cause of death, respectively.23,24

In addition to AMI, we also examined total and cardiovascular mortality (ICD codes 410–414, 431, 433, 434, I21, I22, I25, I61, I63 and I64), stroke (ICD codes 431, 433, 434, I61, I63 and I64), and revascularization procedures (operation codes 3105, 3158, 3127, 3066, 3080, 3092, FNA, FNB, FNC, FNE, FNF, FNG) as secondary outcomes.

Hernia operations—an active control group

To determine whether an association of appendectomy and tonsillectomy with risk of AMI reflected early alteration in the immune system or was simply a correlate of early-life surgery, we also examined the association of a second common surgical exposure—herniorrhaphy—with AMI. We identified all operations owing to inguinal (operation codes JAB00-JAB97 and 4200–4206), femoral (operation codes JAC10-JAC97 and 4210–4213), and umbilical (operation codes JAF10-JAF97 and 4260–4263) hernia in our cohort. For each patient undergoing these operations, we randomly selected five controls without history of the respective operation, matched on sex, age, calendar time, and county of residence. Participants were followed for AMI, as those in the appendectomy and tonsillectomy datasets. We then examined the association between hernia operations and AMI separately for operations occurring before 20 years of age and after that.

Although we are aware of no possible immunological consequences related to hernia repair, hernia and AMI might share common risk factors like obesity25 and smoking.26 However, confounding from these AMI risk factors should be most apparent in adulthood. Therefore, in contrast to our primary hypothesis concerning the removal of MALT tissues, we hypothesized a similar or stronger relation between AMI and hernia operations in adulthood than in childhood.

Family history of acute myocardial infarction

Family history of AMI was defined as fatal or non-fatal AMI of the biological parents. Biological parents were identified by the Swedish Multigeneration Register.

Socioeconomic status

Because participants were chiefly enrolled as young adults, their socioeconomic status was derived from their parents’ occupation. Participants were linked to the parents with whom they lived (biological or adoptive). Information on parents’ socioeconomic position was obtained from the censuses closest to the operation. We identified four occupational categories: manual worker, non-manual worker, self-employed/entrepreneur (including farmers), or other (pensioners, part-time workers, those working at home, or not otherwise classified). Paternal data were preferentially used; if missing, we used maternal data. If socioeconomic information was missing from the census closest to the operation, the next closest census was used.

Statistical analyses

We present unadjusted event-free survival curves constructed using the Kaplan–Meier method and evaluated with the log-rank test. We used Cox proportional hazard models to examine the prospective association between appendectomy, tonsillectomy, and hernia operations and AMI with adjustment for potential confounders. We evaluated the proportionality of hazards using formal two-sided tests of interaction with time or log-time. We found no statistical evidence against the proportionality assumption.

In adjusted analyses, we included matching factors of sex, age (indicators for each birth year), county of residency (25 counties), and date of operation or the corresponding date for controls (5-year categories, i.e. time span closest to a census formed a category). We also further adjusted for parents’ socioeconomic position and family history of AMI. Matched strata analyses yielded very similar results to the inclusion of matching factors in our models.

Because immigrants may have undergone appendectomy or tonsillectomy prior to immigration, we performed sensitivity analyses restricted to those born in Sweden.

We also examined the joint effects of the removal of MALT organs. We combined the samples of individuals with either operation and their respective controls, and specified the number of MALT organs removed from 0 to 2.

Rothman's synergy index with 95% confidence intervals was used to evaluate biological interactions.27 Rothman's synergy index allows assessing effect modification on an additive scale using multiplicative models. A synergy index with a value greater than 1 implies synergism, whereas a value below 1 indicates antagonism between two exposures.

For each analysis, a two-tailed P-value of <0.05 was considered statistically significant.

Statistical analyses were performed using SAS 9 for Windows.

The study was approved by the Karolinska Ethics committee (ethical approval no. 2008/1650–31).

Results

In Table 1, we present characteristics of participants with appendectomy and tonsillectomy before age 20 and their non-exposed controls, respectively. Both operations were more frequent in females than in males. Parents’ occupational position and family history of AMI differed little by operative status.

Table 1

Characteristics of the population with an appendectomy or tonsillectomy <20 years of age and their controls

 Appendectomy Yes Appendectomy No Tonsillectomy Yes Tonsillectomy No 
n 54 449 272 213 27 284 136 401 
 Mean (SD) 
Age at operation/at entry (years) 14.2 (3.6) 14.2 (3.6) 14.9 (3.7) 14.9 (3.7) 
Duration of follow-up (years)a 23.4 (5.9) 23.3 (6.1) 23.5 (6.5) 23.6 (6.3) 
Person-years of follow-up 1 276 058 6 351 485 642 842 3 200 673 

 
 n (%) 
Male sex 25 593 (47.0) 127 945 (47.0) 10 112 (37.1) 50 551 (37.1) 
Parents’ socioeconomic position 
 Manual 22 119 (42.0) 108 282 (41.4) 11 583 (43.9) 55 184 (42.3) 
 Non-manual 21 846 (41.5) 110 960 (42.5) 10 955 (41.5) 54 584 (41.8) 
 Self employed 6300 (12.0) 30 836 (11.8) 2858 (10.8) 15 548 (11.9) 
 Other 2382 (4.5) 11 258 (4.3) 1001 (3.8) 5168 (4.0) 
Family history of AMI 4662 (8.6) 22 340 (8.2) 2535 (9.3) 12 415 (9.1) 
 Appendectomy Yes Appendectomy No Tonsillectomy Yes Tonsillectomy No 
n 54 449 272 213 27 284 136 401 
 Mean (SD) 
Age at operation/at entry (years) 14.2 (3.6) 14.2 (3.6) 14.9 (3.7) 14.9 (3.7) 
Duration of follow-up (years)a 23.4 (5.9) 23.3 (6.1) 23.5 (6.5) 23.6 (6.3) 
Person-years of follow-up 1 276 058 6 351 485 642 842 3 200 673 

 
 n (%) 
Male sex 25 593 (47.0) 127 945 (47.0) 10 112 (37.1) 50 551 (37.1) 
Parents’ socioeconomic position 
 Manual 22 119 (42.0) 108 282 (41.4) 11 583 (43.9) 55 184 (42.3) 
 Non-manual 21 846 (41.5) 110 960 (42.5) 10 955 (41.5) 54 584 (41.8) 
 Self employed 6300 (12.0) 30 836 (11.8) 2858 (10.8) 15 548 (11.9) 
 Other 2382 (4.5) 11 258 (4.3) 1001 (3.8) 5168 (4.0) 
Family history of AMI 4662 (8.6) 22 340 (8.2) 2535 (9.3) 12 415 (9.1) 

AMI, acute myocardial infarction.

aCalculated for censored cases.

Those suffering from AMI during the follow-up were more often male, were more likely to have a family history of AMI, and their parents were more likely to be manual workers (data not shown).

Figures 1 and 2 show the cumulative proportion of surviving patients who did not have an AMI during the follow-up according to appendectomy and tonsillectomy status, respectively. As expected from the young age of studied individuals, the cumulative risk of AMI was low during the study. Evidence of a slightly higher risk among those who had undergone surgery appeared 15–20 years after the index operation (i.e. at age 35 and older). The average age at AMI was 37.9 (4.2) years for men and 37.9 (4.3) for women in the appendectomy dataset. The corresponding numbers were 38.8 (4.6) and 38.7 (4.5) in the tonsillectomy dataset.

Figure 1

Life tables and Kaplan–Meier plots for cumulative proportion of surviving patients who did not have an acute myocardial infarction during the follow-up in relation to appendectomy <20 years of age at the time of surgery (P for log-rank test = 0.012).

Figure 1

Life tables and Kaplan–Meier plots for cumulative proportion of surviving patients who did not have an acute myocardial infarction during the follow-up in relation to appendectomy <20 years of age at the time of surgery (P for log-rank test = 0.012).

Figure 2

Life tables and Kaplan–Meier plots for cumulative proportion of surviving patients who did not have an acute myocardial infarction during the follow-up in relation to tonsillectomy <20 years of age at the time of surgery (P for log-rank test = 0.047).

Figure 2

Life tables and Kaplan–Meier plots for cumulative proportion of surviving patients who did not have an acute myocardial infarction during the follow-up in relation to tonsillectomy <20 years of age at the time of surgery (P for log-rank test = 0.047).

As seen in Table 2, appendectomy and tonsillectomy occurring before 20 years of age were associated with an increased relative risk for AMI. Adjustment for matching factors, parental socioeconomic status, and family history of AMI had little influence on the point estimates (Table 2).

Table 2

Appendectomy, tonsillectomy, and risk for AMI among Swedish individuals <20 years of age at the time of surgery

 Total (nAMI (nUnadjusted HR (95% CI) Matching adjusteda HR (95% CI) Multiadjustedb HR (95% CI) 
No appendectomy 272 213 328 
Appendectomy 54 449 89 1.35 (1.07–1.71) 1.35 (1.07–1.70) 1.33 (1.05–1.70) 
No tonsillectomy 136 401 169 
Tonsillectomy 27 284 47 1.39 (1.00–1.91) 1.39 (1.00–1.91) 1.44 (1.04–2.01) 
 Total (nAMI (nUnadjusted HR (95% CI) Matching adjusteda HR (95% CI) Multiadjustedb HR (95% CI) 
No appendectomy 272 213 328 
Appendectomy 54 449 89 1.35 (1.07–1.71) 1.35 (1.07–1.70) 1.33 (1.05–1.70) 
No tonsillectomy 136 401 169 
Tonsillectomy 27 284 47 1.39 (1.00–1.91) 1.39 (1.00–1.91) 1.44 (1.04–2.01) 

95% CI indicates 95% confidence interval; HR, hazard ratio, AMI, acute myocardial infarction.

aMatching criteria included sex, age (dummies for each birth year), county of residency (25 counties), and time of operation or the corresponding time for controls (5-year categories, i.e. time span closest to a census formed a category).

bAdjusted for matching criteria + parents’ socioeconomic position and family history of AMI.

As a sensitivity analysis, we restricted our analyses to those who were born in Sweden, eliminating 17 805 immigrants from the appendectomy dataset and 8734 from the tonsillectomy dataset. The results were similar, with adjusted HRs of 1.35 (1.06–1.74) for appendectomy and 1.52 (1.09–2.11) for tonsillectomy.

When we analysed appendectomy and tonsillectomy together, the adjusted HR for the increase in risk with each operation was 1.34 (1.10–1.63). When we analysed the effect of appendectomy only (n = 52 767), tonsillectomy only (n = 25 602), and the effect of both operations (n = 1682) compared with those who had none of these operations, the adjusted HRs were 1.33 (1.04–1.70) for appendectomy, 1.29 (0.94–1.78) for tonsillectomy, and 2.42 (0.78–7.57) for both. The synergy index for the interaction between the two operations was 2.27 (0.28–18.51).

We found no evidence that secular trends modified the observed associations. For example, the HR for each additional operation was 1.33 (1.05–1.68) for operations up to the census in 1975 and 1.35 (0.92–1.99) for those occurring afterwards.

Appendectomies coded with different accompanying conditions had very similar associations with the risk of AMI. The adjusted HRs for appendectomy owing to perforated appendicitis/appendicitis with abscess, for appendectomy owing to appendicitis without perforation or abscess, and for appendectomy without appendicitis were 1.29 (0.62–2.69, n = 29 228), 1.31 (0.96–1.80, n = 20 7213), and 1.39 (0.90–2.14, n = 90 221), respectively.

We next examined risk stratified by sex. In the case of appendectomy, the associations were similar when men and women were analysed separately. Tonsillectomy had a somewhat stronger association with AMI in men than in women. The adjusted HRs were 1.65 (1.09–2.50) for males and 1.18 (0.68–2.03) for females. The synergy index for the interaction between male gender and tonsillectomy was 1.91 (0.87–4.19).

We also analysed total and cardiovascular mortality, stroke, and coronary revascularization procedures as secondary outcomes. We observed a total of 5594 (289 classified as cardiovascular) and 2794 (159 classified as cardiovascular) deaths in the appendectomy and tonsillectomy datasets, respectively. A total of 615 and 354 participants had stroke and 244 and 111 participants underwent coronary revascularizations in the appendectomy and tonsillectomy datasets, respectively. Both appendectomy and tonsillectomy were associated with an increased risk for total mortality, stroke, and coronary revascularization procedures, but the associations with cardiovascular mortality were less consistent. The multi-adjusted HRs associated with appendectomy were 1.10 (1.02–1.18) for total mortality, 0.85 (0.61–1.20) for cardiovascular mortality, 1.30 (1.06–1.59) for stroke, and 1.47 (1.08–2.00) for revascularization procedures. The corresponding numbers for tonsillectomy were 1.11 (1.00–1.22), 1.40 (0.95–2.06), 1.12 (0.85–1.48), and 1.47 (0.93–2.31), respectively.

Consistent with our hypotheses, there was no evidence for an association between appendectomy or tonsillectomy performed ≥20 years of age with AMI in any of the models. For example, in models adjusted for the matching criteria, parents' socioeconomic position, and family history of AMI, the HRs for appendectomy and tonsillectomy were 1.04 (0.85–1.28) and 0.90 (0.66–1.23), respectively. In contrast, when we restricted to operations before age 15, we observed similar results with somewhat higher effect sizes but lower precision than for the main analyses (i.e. restricted to operations before age 20). The HRs were 1.60 (1.13–2.27) for appendectomy and 1.55 (0.90–2.68) for tonsillectomy in these secondary analyses.

We also followed patients who underwent hernia operations and their controls for AMI. The HR for AMI was 1.30 (0.75–2.25) in the fully adjusted model for hernia operations occurring before 20 years of age, with a total of 79 cases of AMI among 5550 participants. For operations at 20 years of age and after, the corresponding HR was 1.42 (1.01–1.99) with a total of 228 cases of AMI among 80 884 participants.

Discussion

In two population-based cohorts comprising over 400 and 200 cases of AMI accruing over 7.5 million and nearly 4 million person-years of follow-up, respectively, we found that appendectomy and tonsillectomy, when performed before adulthood, were associated with a moderately increased relative risk for subsequent AMI. This association was graded, with the highest risk among those undergoing both procedures, and was consistent in both sexes. As expected from the young age of the population, the observed moderate increases in relative risk corresponded to small risk increases in absolute terms.

Our analyses of secondary outcomes, i.e. total mortality, stroke, and revascularization procedures generally support the hypothesis that appendectomy and tonsillectomy may have subtle effects that increase the risk of CHD. However, the association between appendectomy and tonsillectomy and cardiovascular mortality were less consistent. Apart from the relatively low statistical power to examine cardiovascular mortality, all-cause mortality, revascularization procedures, and AMI are identifiable with greater reliability than cause-specific mortality, and hence, non-differential misclassification might have diluted the observed effects. Alternatively, it is possible but seems less likely that removal of mucosa associated tissues influence cardiovascular risk and prognosis differently.

We found that only operations that occurred before age 20 were associated with higher risk for AMI. Because these operations occurred in childhood and adolescence, traditional risk factors such as diabetes or obesity are not likely to confound these relationships unless these risk factors are associated more strongly or exclusively with operations occurring in children and in teens compared with later operations. This substantially limits the range of potential confounders under consideration. Furthermore, the observed associations remained after adjustment for age, sex, county of residency, calendar time of operation, parental history of MI, and parental socioeconomic status. Any remaining potentially confounding risk factor would need to be associated preferentially with operations occurring in children and teens and generally independent of the factors included in our multivariable models.

In contrast to our findings concerning appendectomy and tonsillectomy and AMI risk, we found that hernia operations were associated similarly and at least as strongly with AMI if performed in adulthood than if performed in childhood. This may suggest that common AMI risk factors like obesity or smoking25,26 could confound the relationship between hernia operations with AMI, and that this confounding is at least as strong in adulthood than in childhood. These differences between hernia repair and appendectomy/tonsillectomy—with weaker effects with older age only for the latter—are consistent with our original hypotheses, but ultimately larger studies with greater numbers of endpoints will be needed to test these issues definitively.

An intriguing and biologically plausible explanation for our findings is the possibility that the alteration in immune function mediates the effects of the operations on AMI risk. The appendix and tonsils are secondary lymphoid organs. Their removal can affect several aspects of immune activity, including decreased production of immunoglobulins, especially that of immunoglobulin A.4,14 This effect seems to be most pronounced when both appendix and tonsils are removed.4

Although relatively few long-term health consequences are clearly established for appendectomy and tonsillectomy, they may specifically alter the risk for diseases where the immune system plays a key role. Several studies suggested that Hodgkin's lymphoma is associated with appendectomy and/or tonsillectomy.10,15 Appendectomy and tonsillectomy are risk factors for Crohn's disease,17,18 and appendectomy appears to protect against ulcerative colitis.11–13 Appendectomy and tonsillectomy may be a risk factor for rheumatoid arthritis as well.16 Notably, in studies where effect modification by age was considered, these associations seem to be restricted mainly or exclusively to those individuals in whom the appendix and/or the tonsils are removed before adulthood.10–12 This concords with the fact that the lymphoid mass and function of these organs are most pronounced between 10 and 20 years of age and markedly decrease in adulthood.1,2

Atherosclerosis, the underlying pathophysiological mechanism behind AMI, is widely considered to be an inflammatory process. Trapped and oxidatively modified LDL (oxLDL), other autoantigens, and perhaps select microbial pathogens may provoke an immune response.20,28,29 Several autoimmune diseases and disorders involving chronic inflammation are associated with an increased risk for CHD.30,31 Epidemiological studies clearly demonstrate that C-reactive protein and other inflammatory markers also predict the risk of CHD nearly as well as more traditional risk factors.20,28,32 Even mild elevations in C-reactive protein level, previously considered in the ‘normal range’, are positively associated with cardiovascular events.28

The role of the immune system is complex in atherosclerosis. Some facets of vascular immunity protect against atherosclerosis rather than facilitate it.28 For example immunization with oxLDL reduces atherogenesis in animal experiments.33 Several animal experiments and human studies suggest that humoral immunity can protect against atherosclerosis.28 B-cell deficiency is associated with more pronounced lesions in animal models of atherosclerosis.34 Removal of the spleen—another secondary lymphoid organ, although not part of the MALT system—is associated with an accelerated atherosclerosis both in experimental animals35 and in humans.36 Thus, we hypothesize that the removal of MALT organs might alter atheroprotective immunity. Alternatively, these operations may decrease the capacity of the immune system to eliminate external pathogens and thus lead to increased risk for atherosclerosis. Increased risk of autoimmune disorders, like rheumatoid arthritis, could also be one of the possible pathways of how childhood appendectomy and tonsillectomy might lead to AMI.

Our analyses on the joint effects of the removal of both MALT organs suggested that the joint effect was more pronounced than the independent effects of the two operations. However, our statistical power to detect this interaction was limited. Our results also indicated that there might be a synergism between the effect of male gender and tonsillectomy on AMI risk. In line with this finding, limited evidence suggests that the immunological effects of the removal of tonsils is more pronounced among boys.14

Frequently, appendices are removed when operations in the right lower quadrant are performed, on the presumption that treating practitioners in later life will assume that an appendectomy had been performed. Moreover, recent research suggests that not all cases of appendicitis require surgery, as some patients may resolve spontaneously or could be treated with antibiotics alone.37 Indications for tonsillectomy are also controversial.5,38 Our findings on increased risk for AMI in association with these operations when performed before age 20—if confirmed and the absolute risk associated with them better established—suggest that the full late burden of illness attributable to these operations, potentially including AMI and autoimmune disorders, may need to be considered during the decision-making process for more elective procedures.

Despite its large size, long duration of follow-up, and population-based design, our study is subjected to important limitations. We relied on Swedish health and administrative registers that provide high-quality information with a high degree of reliability and with complete coverage across Sweden.39 Nonetheless, the registration of hospital diagnoses started only in 1964, and it did not fully cover all counties of Sweden until 1 January 1987. Because participants were still relatively young in 1987, it is unlikely that we missed substantial numbers of cases of AMI, but we certainly missed some appendectomies and tonsillectomies. To account for this, we matched exposed and non-exposed participants on the county of residence and calendar time, which reflect the probability of being labelled as exposed conditional upon true exposure. More importantly, because this type of exposure misclassification simply makes the non-exposed group more similar to those exposed, it cannot lead to a false finding of association and instead probably have led us to underestimate the true association.

One of our important limitations was that we studied a childhood exposure, with the consequence that our study population was relatively young even at the end of the follow-up. Consequently, we cannot directly extrapolate our findings to cases of AMI that occur among older men or women, in whom risk is highest. However, we found no evidence that risk attenuated over time within the limits of follow-up duration of our study.

As with any observational study, confounding factors for which we did not adjust could explain our findings. For example, low fibre intake1 and smoking40 might increase the risk for appendicitis. We could not control for these factors, although socioeconomic status (for which we did adjust) is relatively strongly related to both fibre intake41 and smoking.42 Moreover, as highlighted above, it is not clear how such potential confounders could explain why only operations before age 20 were associated with the risk of AMI.

Conclusion

In this prospective matched cohort study, we found an elevated risk for AMI related to the removal of tonsils and appendix before age 20. We hypothesize that subtle alterations in immune function owing to these operations may underlie this relationship. However, further studies are needed to confirm these findings, particularly in cohort studies with characterization of immune and inflammatory status and with information on all cardiovascular risk factors that may present in childhood.

Funding

The study was supported by the Swedish Council of Working Life and Social Research and by the Ansgarius Foundation.

Conflict of interest: none declared.

References

1
Prystowsky
JB
Pugh
CM
Nagle
AP
Current problems in surgery. Appendicitis
Curr Probl Surg
 , 
2005
, vol. 
42
 (pg. 
688
-
742
)
2
Siegel
G
Description of age-depending cellular changes in the human tonsil
ORL J Otorhinolaryngol Relat Spec
 , 
1978
, vol. 
40
 (pg. 
160
-
171
)
3
Farstad
IN
Halstensen
TS
Kvale
D
Fausa
O
Brandtzaeg
P
Topographic distribution of homing receptors on B and T cells in human gut-associated lymphoid tissue: relation of L-selectin and integrin alpha 4 beta 7 to naive and memory phenotypes
Am J Pathol
 , 
1997
, vol. 
150
 (pg. 
187
-
199
)
4
Andreu-Ballester
JC
Perez-Griera
J
Ballester
F
Colomer-Rubio
E
Ortiz-Tarin
I
Penarroja Otero
C
Secretory immunoglobulin A (sIgA) deficiency in serum of patients with GALTectomy (appendectomy and tonsillectomy)
Clin Immunol
 , 
2007
, vol. 
123
 (pg. 
289
-
297
)
5
Brandtzaeg
P
Immunology of tonsils and adenoids: everything the ENT surgeon needs to know
Int J Pediatr Otorhinolaryngol
 , 
2003
, vol. 
67
 
Suppl 1
(pg. 
S69
-
76
)
6
Derkay
CS
Pediatric otolaryngology procedures in the United States: 1977–1987
Int J Pediatr Otorhinolaryngol
 , 
1993
, vol. 
25
 (pg. 
1
-
12
)
7
Addiss
DG
Shaffer
N
Fowler
BS
Tauxe
RV
The epidemiology of appendicitis and appendectomy in the United States
Am J Epidemiol
 , 
1990
, vol. 
132
 (pg. 
910
-
925
)
8
Lee
JH
Park
YS
Choi
JS
The epidemiology of appendicitis and appendectomy in South Korea: national registry data
J Epidemiol/Jpn Epidemiologic Assoc
 , 
2010
, vol. 
20
 (pg. 
97
-
105
)
9
Vestergaard
H
Wohlfahrt
J
Westergaard
T
Pipper
C
Rasmussen
N
Melbye
M
Incidence of tonsillectomy in Denmark, 1980 to 2001
Pediatr Infect Dis J
 , 
2007
, vol. 
26
 (pg. 
1117
-
1121
)
10
Liaw
KL
Adami
J
Gridley
G
Nyren
O
Linet
MS
Risk of Hodgkin's disease subsequent to tonsillectomy: a population-based cohort study in Sweden
Int J Cancer
 , 
1997
, vol. 
72
 (pg. 
711
-
713
)
11
Andersson
RE
Olaison
G
Tysk
C
Ekbom
A
Appendectomy and protection against ulcerative colitis
N Engl J Med
 , 
2001
, vol. 
344
 (pg. 
808
-
814
)
12
Frisch
M
Pedersen
BV
Andersson
RE
Appendicitis, mesenteric lymphadenitis, and subsequent risk of ulcerative colitis: cohort studies in Sweden and Denmark
Br Med J
 , 
2009
, vol. 
338
 pg. 
b716
 
13
Matsushita
M
Uchida
K
Okazaki
K
Role of the appendix in the pathogenesis of ulcerative colitis
Inflammopharmacology
 , 
2007
, vol. 
15
 (pg. 
154
-
157
)
14
Bock
A
Popp
W
Herkner
KR
Tonsillectomy and the immune system: a long-term follow up comparison between tonsillectomized and non-tonsillectomized children
Eur Arch Otorhinolaryngol
 , 
1994
, vol. 
251
 (pg. 
423
-
427
)
15
Cozen
W
Hamilton
AS
Zhao
P
Salam
MT
Deapen
DM
Nathwani
BN
Weiss
LM
Mack
TM
A protective role for early oral exposures in the etiology of young adult Hodgkin lymphoma
Blood
 , 
2009
, vol. 
114
 (pg. 
4014
-
4020
)
16
Fernandez-Madrid
F
Reed
AH
Karvonen
RL
Granda
JL
Influence of antecedent lymphoid surgery on the odds of acquiring rheumatoid arthritis
J Rheumatol
 , 
1985
, vol. 
12
 (pg. 
43
-
48
)
17
Andersson
RE
Olaison
G
Tysk
C
Ekbom
A
Appendectomy is followed by increased risk of Crohn's disease
Gastroenterology
 , 
2003
, vol. 
124
 (pg. 
40
-
46
)
18
Gearry
RB
Richardson
AK
Frampton
CM
Dodgshun
AJ
Barclay
ML
Population-based cases control study of inflammatory bowel disease risk factors
J Gastroenterol Hepatol
 , 
2010
, vol. 
25
 (pg. 
325
-
333
)
19
Ludvigsson
JF
Askling
J
Ekbom
A
Montgomery
SM
Diagnosis underlying appendectomy and coeliac disease risk
Dig Liver Dis
 , 
2006
, vol. 
38
 (pg. 
823
-
828
)
20
Hansson
GK
Inflammation, atherosclerosis, and coronary artery disease
N Engl J Med
 , 
2005
, vol. 
352
 (pg. 
1685
-
1695
)
21
Dalman
C
Allebeck
P
Gunnell
D
Harrison
G
Kristensson
K
Lewis
G
Lofving
S
Rasmussen
F
Wicks
S
Karlsson
H
Infections in the CNS during childhood and the risk of subsequent psychotic illness: a cohort study of more than one million Swedish subjects
Am J Psychiatry
 , 
2008
, vol. 
165
 (pg. 
59
-
65
)
22
Forsell
Y
Dalman
C
Wicks
S
Jögensen
L
Forsner
T
Mattsson
M
Cullberg
J
Airaksinen
E
Metoder för att mäta och analysera psykisk sjuklighet i befolkningen [Methods for measuring and analyzing mental morbidity in the population, in Swedish]
Socialmedicinsk Tidskrift
 , 
2009
, vol. 
2
 (pg. 
139
-
145
)
23
Hjärtinfarkter 1987–2007
Myocardial infarctions in Sweden 1987–2007
 , 
2009
Stockholm
Official Statistics of Sweden Statistics—Health and Medical Care
24
Hammar
N
Nerbrand
C
Ahlmark
G
Tibblin
G
Tsipogianni
A
Johansson
S
Wilhelmsen
L
Jacobsson
S
Hansen
O
Identification of cases of myocardial infarction: hospital discharge data and mortality data compared with myocardial infarction community registers
Int J Epidemiol
 , 
1991
, vol. 
20
 (pg. 
114
-
120
)
25
Wellman
NS
Friedberg
B
Causes and consequences of adult obesity: health, social and economic impacts in the United States
Asia Pac J Clin Nutr
 , 
2002
, vol. 
11
 
Suppl. 8
(pg. 
S705
-
709
)
26
Sorensen
LT
Friis
E
Jorgensen
T
Vennits
B
Andersen
BR
Rasmussen
GI
Kjaergaard
J
Smoking is a risk factor for recurrence of groin hernia
World J Surg
 , 
2002
, vol. 
26
 (pg. 
397
-
400
)
27
Lundberg
M
Fredlund
P
Hallqvist
J
Diderichsen
F
A SAS program calculating three measures of interaction with confidence intervals
Epidemiology
 , 
1996
, vol. 
7
 (pg. 
655
-
656
)
28
Hansson
GK
Libby
P
The immune response in atherosclerosis: a double-edged sword
Nat Rev Immunol
 , 
2006
, vol. 
6
 (pg. 
508
-
519
)
29
Nilsson
J
Hansson
GK
Autoimmunity in atherosclerosis: a protective response losing control?
J Intern Med
 , 
2008
, vol. 
263
 (pg. 
464
-
478
)
30
Buhlin
K
Gustafsson
A
Ahnve
S
Janszky
I
Tabrizi
F
Klinge
B
Oral health in women with coronary heart disease
J Periodontol
 , 
2005
, vol. 
76
 (pg. 
544
-
550
)
31
Riboldi
P
Gerosa
M
Luzzana
C
Catelli
L
Cardiac involvement in systemic autoimmune diseases
Clin Rev Allergy Immunol
 , 
2002
, vol. 
23
 (pg. 
247
-
261
)
32
Ridker
PM
Hennekens
CH
Buring
JE
Rifai
N
C-reactive protein and other markers of inflammation in the prediction of cardiovascular disease in women
N Engl J Med
 , 
2000
, vol. 
342
 (pg. 
836
-
843
)
33
Palinski
W
Miller
E
Witztum
JL
Immunization of low density lipoprotein (LDL) receptor-deficient rabbits with homologous malondialdehyde-modified LDL reduces atherogenesis
Proc Natl Acad Sci USA
 , 
1995
, vol. 
92
 (pg. 
821
-
825
)
34
Major
AS
Fazio
S
Linton
MF
B-lymphocyte deficiency increases atherosclerosis in LDL receptor-null mice
Arterioscler Thromb Vasc Biol
 , 
2002
, vol. 
22
 (pg. 
1892
-
1898
)
35
Caligiuri
G
Nicoletti
A
Poirier
B
Hansson
GK
Protective immunity against atherosclerosis carried by B cells of hypercholesterolemic mice
J Clin Invest
 , 
2002
, vol. 
109
 (pg. 
745
-
753
)
36
Robinette
CD
Fraumeni
JF
Jr.
Splenectomy and subsequent mortality in veterans of the 1939–45 war
Lancet
 , 
1977
, vol. 
2
 (pg. 
127
-
129
)
37
Mason
RJ
Surgery for appendicitis: is it necessary?
Surg Infect (Larchmt)
 , 
2008
, vol. 
9
 (pg. 
481
-
488
)
38
Munir
N
Clarke
R
Indications for tonsillectomy: the evidence base and current UK practice
Br J Hosp Med (Lond)
 , 
2009
, vol. 
70
 (pg. 
344
-
347
)
39
Rosen
M
National Health Data Registers: a Nordic heritage to public health
Scand J Public Health
 , 
2002
, vol. 
30
 (pg. 
81
-
85
)
40
Montgomery
SM
Pounder
RE
Wakefield
AJ
Smoking in adults and passive smoking in children are associated with acute appendicitis
Lancet
 , 
1999
, vol. 
353
 pg. 
379
 
41
Shoaf
LR
Health awareness and life-style practices of employees in extended-care facilities
J Am Diet Assoc
 , 
1986
, vol. 
86
 (pg. 
1693
-
1697
)
42
Lindstrom
M
Moghaddassi
M
Bolin
K
Lindgren
B
Merlo
J
Social participation, social capital and daily tobacco smoking: a population-based multilevel analysis in Malmo, Sweden
Scand J Public Health
 , 
2003
, vol. 
31
 (pg. 
444
-
450
)

Supplementary data

Comments

2 Comments
Childhood appendectomy, tonsillectomy, and risk for premature acute myocardial infarction--a nationwide population-based cohort study
19 June 2011
Simon S. M. Ho (with Mr Matthew Rollin, Prof Tony Narula)

Dear Editor,

We read with interest the recent paper by Janszky et al (1), however we have some concerns regarding the results and conclusions of this paper. The authors report an increased risk of acute myocardial infarction in patients who underwent appendicectomy or tonsillectomy before 20 years of age, and concluded that this finding would be consistent with the hypothesis that alterations in immune function by these procedures increase the risk of ischaemic heart disease.

We are unable to identify the a priori reasoning which lies behind this hypothesis, and are concerned that the hypothesis itself may unfortunately represent some measure of post-hoc rationalisation. In addition, the data as published does not appear sufficient to support even this reasoning: using the published data, we have calculated an absolute risk increase of 0.048% for acute myocardial infarction after tonsillectomy compared to the control population, which corresponds to a number needed to harm of 2068.

The authors also stated that atherosclerosis is widely considered to be an inflammatory process and pathogens may provoke an immune response (1). As both appendicitis and recurrent tonsillitis can cause local and systemic inflammatory changes and recurring bacteraemia, it is entirely possible that any increased risk of cardiac disorder may be related to these disorders themselves rather than surgical management of them.

We believe that the authors' hypothesis might be better tested if the effects of removal of other secondary non-infected lymphoid organs such as the spleen were also studied. The authors suggest that the increase in risk of acute myocardial infarction should be considered during the decision-making process for tonsillectomy. There is strong evidence supporting the efficacy of this procedure for its two main indications of recurrent tonsillitis (2) and obstructive sleep apnoea (3); and good supporting evidence that untreated, these conditions can lead to long-term cardiovascular complications, including (in the case of sleep-disordered breathing) pulmonary hypertension (4).

Given the extremely small increase in absolute risk, we are concerned that the authors' conclusion will lead to quite unnecessary levels of anxiety, and the unfortunate possibility that surgical procedures necessary to reduce genuine long-term risks may not be undertaken.

Dr Simon Ho, Mr Matthew Rollin, Prof Tony Narula

References 1. Janszky I, Mukamal KJ, Dalman C, Hammar N, Ahnve S. Childhood appendectomy, tonsillectomy, and risk for premature acute myocardial infarction--a nationwide population-based cohort study. Eur Heart J. Advance Access published online June 1, 2011 doi:10.1093/eurheartj/ehr137 2. Goldstein NA, Stewart MG, Witsell DL, Hannley MT, Weaver EM, Yueh B, Smith TL, Orvidas LJ. Quality of life after tonsillectomy in children with recurrent tonsillitis. Otolaryngol Head Neck Surg;138:S9-S16. 3. Georgalas C, Barbar-Craig H, Arora A, Narula A. Health outcome measurements in children with sleep disordered breathing. Clin Otolaryngol 2007;32:268-274. 4. Nixon GM, Brouillette RT. Sleep - 8: Paediatric obstructive sleep apnoea. Thorax 2005;60:511-516.

Conflict of Interest:

None declared

Submitted on 19/06/2011 8:00 PM GMT
Reply
19 June 2011
Imre Janszky (with Kenneth J. Mukamal, Christina Dalman, Niklas Hammar, Staffan Ahnve)

We thank Professors Ho, Rollin and Narula for their interest in our work. Here we clarify a few issues.

First, we clearly state that our findings should only be used in clinical decision-making "if our findings are confirmed and the absolute risk associated with them better established." Since that is not yet the case, we do not recommend these findings be used in such a manner currently.

We clearly noted that the risk associated with these operations was small in absolute terms. The small absolute risk is simply attributable to the young age of the participants. Due to this, any risk factor will confer a small absolute risk. That has no bearing on the plausibility of our findings nor the likelihood of a causal relationship.

The authors posit that inflammation from appendicitis and tonsillitis per se could lead to coronary heart disease. We see no reasonable mechanism by which local inflammation caused by appendicitis and/or tonsillitis in childhood could itself lead to myocardial infarction literally decades after the removal of appendix and/or the tonsils.

They also suggest examining splenectomy, but it has already been found to be associated with increased progression of atherosclerosis and an increased risk for acute myocardial infarction both in animal and human studies (Caligiuri, Nicoletti, Poirier, & Hansson, 2002; Robinette & Fraumeni, 1977), consistent with our findings for removal of mucosa- associated lymphoid tissue.

Finally, the authors suggest that the hypothesis represents "post-hoc rationalisation". Long before we assembled these data, previous studies found an altered risk for other diseases of immune origin in association with these operations in childhood. We applied for ethics review board permission to address this very specific research question more than a year before we were able to analyze the data. Moreover, the design of our study, in which we derived a specific study cohort, required that we had to prespecify the exposure and match non-exposed participants before analysis. Post-hoc exploration of data was simply impossible.

Nevertheless, we emphasize again that further studies are needed to confirm our findings and to explore the mechanism behind the observed association.

Caligiuri, G., Nicoletti, A., Poirier, B., & Hansson, G. K. (2002). Protective immunity against atherosclerosis carried by B cells of hypercholesterolemic mice. J Clin Invest, 109(6), 745-753. Robinette, C. D., & Fraumeni, J. F., Jr. (1977). Splenectomy and subsequent mortality in veterans of the 1939-45 war. Lancet, 2(8029), 127- 129.

Conflict of Interest:

None declared

Submitted on 19/06/2011 8:00 PM GMT