A novel technology for disinfecting surfaces infested with Candida auris: the UVC chip

Abstract Background The fungal pathogen Candida Auris is increasingly associated with multidrug-resistant infections that are highly expensive for the Health Care System. The spreading of this pathogen can occur, among others, through contact with infected surfaces or medical instruments. This study evaluated the efficacy of a novel UVC chip, novel alternative to UVC LEDs and lamps, in inactivating Candida auris strain. Methods This experimental study was carried out between July and September 2020 at the University of Siena. Candida auris (ATCC 12372) at two known concentrations (1.5X107 and 1.5x106 CFU/ml) at a fixed distance (7,5 cm) from the chip (5.1mW radiant power) was tested, in triplicates, with three exposure times (5, 10 and 15 minutes). Potato Dextrose Agar (PDA) plates without the plate lid and containing Candida auris were exposed to UVC light. Subsequently, the plates were incubated at 36 °C for 48 h. Log reduction between treated and positive control (not exposed to UVC light) samples was calculated. Results At 15 minutes, we had the highest inactivation result, mean 4.43 log10, starting from a 1.5x106 CFU/mL concentration. At a higher concentration, 1.5X107 CFU/mL, the reduction had a mean of 3.51 log10. Conclusions The results of the experiments showed a significant microbial reduction in relation to the exposure time. The highest level of reduction was reached after 15 minutes of exposure. UVC chip had a relevant biocidal effect on Candida auris and may represent a valuable tool in the prevention of infections caused by this pathogen, which is becoming increasingly prevalent and persistent globally. Key messages • The use of UVC Chip decreases surface contamination. • New technology against healthcare-associated infections.


Introduction:
CoViD19 pandemic highlighted the importance of air purifiers and, in commercialization, their performance and price influence the choice.Since primary focus concerns only performance in terms of CADR (Clean Air Delivery Rate), this study aims to compare: I) levels of declared air purifications according to different types of air purification technologies; II) price of them to evaluate if, with similar group-mean CADR (within +/-1 SD), there are significant differences in selling prices.

Methods:
A review of several devices was carried out, collecting data in January-April 2022.Four different types of air purifiers were considered, divided into as many groups: those equipped with HEPA filters + UV lamps, only with HEPA filters, only with UV lamps and those using other technologies.We applied Kruskal-Wallis test to evaluate statistical differences among prices normalized by CADR, at significant level of 0.05.

Conclusions:
Comparison between technologies analyzed by mean price normalized to CADR showed significant differences between those that used only UV lamps compared to all the others.This is reasonably due to the fact that the use of only UV lamps requires radiant powers considerably greater than all the others, therefore also higher costs (about 5-6 times).In all cases, the level of disinfection reached, as declared, was always > 95%.

Key messages:
With the same mean price normalized to CADR, the selling price is significanly different only for devices equipped with UV lamps compared to all the others.Choice of devices with a certain level of declared air purifications can be directed towards those with HEPA+UV/ HEPA/other without the mean price normalized to CADR undergoing significant differences.
Abstract citation ID: ckac131.251A novel technology for disinfecting surfaces infested with Candida auris: the UVC chip

Background:
The fungal pathogen Candida Auris is increasingly associated with multidrug-resistant infections that are highly expensive for the Health Care System.The spreading of this pathogen can occur, among others, through contact with infected surfaces or medical instruments.This study evaluated the efficacy of a novel UVC chip, novel alternative to UVC LEDs and lamps, in inactivating Candida auris strain.
Methods: This experimental study was carried out between July and September 2020 at the University of Siena.Candida auris (ATCC 12372) at two known concentrations (1.5X107 and 1.5x106 CFU/ml) at a fixed distance (7,5 cm) from the chip (5.1mW radiant power) was tested, in triplicates, with three exposure times (5, 10 and 15 minutes).Potato Dextrose Agar (PDA) plates without the plate lid and containing Candida auris were exposed to UVC light.Subsequently, the plates were incubated at 36 C for 48 h.Log reduction between treated and positive control (not exposed to UVC light) samples was calculated.

Results:
At 15 minutes, we had the highest inactivation result, mean 4.43 log10, starting from a 1.5x106 CFU/mL concentration.At a higher concentration, 1.5X107 CFU/mL, the reduction had a mean of 3.51 log10.

Conclusions:
The results of the experiments showed a significant microbial reduction in relation to the exposure time.The highest level of reduction was reached after 15 minutes of exposure.UVC chip had a relevant biocidal effect on Candida auris and may represent a valuable tool in the prevention of infections caused by this pathogen, which is becoming increasingly prevalent and persistent globally.

Key messages:
The use of UVC Chip decreases surface contamination.New technology against healthcare-associated infections.
Abstract citation ID: ckac131.252Does Portuguese pharmaceutical market follow an innovative trend?The INFOMED database analysis Fernando Genovez AvelarFG Avelar 1 , B Raposo 1 , A Torres 1 , AR Pedro 1,2 1 Public Health Research Centre, National School of Public Health, Lisbon, Portugal 15th European Public Health Conference 2022 iii513