Summary

Exposure of dry oxic paddy sol to submerged anoxic conditions resulted in a decrease of the soil redox potential and initiation of CH4 production. However, the most probable numbers (MPN) of hydrogenotrophic and acetotrophic methanogens did not change upon flooding the soil and upon initiation of CH4 production, and stayed constant at 105−106 and 104−105 g−1 d.w. soil, respectively. Cells of Methanobacterium strain Bab1 added to anoxic soil suspensions mostly survived drying and storage under an air atmosphere. Initiation of CH4 production was not affected by storage of dry soil under N2, air, or O2 atmosphere. However, it was negatively affected by storage under the air at high negatively affected by storage under air at high relative humidities, under moist conditions, or by using only the small (< 100 μ m) soil particle fraction. All these treatments also resulted in decreased contents of dissolved organic carbon (DOC). The soil's capacity for initiation of CH4 production could be regenerated either by addition of glucose or by exposure to H2 and addition of reducing agents. The establishment of decreased redox potentials and the provision of methanogenic substrates by heterotrophic bacteria, but not the number of methanogenic bacteria, seemed to be the essential factors for initiation of CH4 production after submergence of oxic paddy soil. CH4 production could also be initiated in forest and arable soils. Here, however, the population of methanogenic bacteria (MPN) increased from about 103 to 105−106 g−1 d.w. when methanogenesis started.

References

[1]
Seiler
W.
(
1985
)
Contribution of biological processes to the global budget of CH4 in the atmosphere
In
Current Perspectives in Microbiological Ecology
 
Klug
M.J.
Reddy
C.A.
, Eds) pp.
468
477
American Society of Microbiology
,
Washington, D.C.
.
[2]
Cicerone
R.J.
Oremland
R.S.
(
1988
)
Biochemical aspects of atmospheric methane
Glob. Biogeochem. Cycles
 ,
2
,
299
327
.
[3]
Holzapfel-Pschorn
A.
Seiler
W.
(
1986
)
Methane emission during a cultivation period from an Italian rice paddy
J. Geophys. Res.
 ,
91
,
11803
11814
.
[4]
Yamane
I.
Sato
K.
(
1963
)
Decomposition of organic acids and gas formation in flooded soil
Soil Sci. Plant Nutr.
 ,
9
,
32
36
.
[5]
Yamane
I.
Sato
K.
(
1964
)
Decomposition of glucose and gas formation in flooded soil
Soil Sci. Plant Nutr.
 ,
10
,
35
41
.
[6]
Yamane
I.
Sato
K.
(
1967
)
Effect of temperature on the decomposition of organic substances in flooded soil
Soil Sci. Plant Nutr.
 ,
13
,
94
100
.
[7]
Watanabe
I.
Furusaka
C.
(
1980
)
Microbial ecology of flooded rice soils
Adv. Microb. Ecol.
 ,
4
,
125
168
.
[8]
Inubishi
K.
Wada
H.
Takai
Y.
(
1984
)
Easily decomposable organic matter in paddy soil. IV. Relationship betwen reduction process and organic matter decomposition
Soil Sci. Plant Nutr.
 ,
30
,
189
198
.
[9]
Ponnamperuma
F.N.
(
1972
)
The chemistry of submerged soils
Adv. Agron.
 ,
24
,
29
96
.
[10]
Mah
R.A.
Ward
D.M.
Baresi
L.
Glass
T.L.
(
1977
)
Biogenesis of methane
Ann. Rev. Microbiol.
 ,
31
,
309
341
.
[11]
Kiener
A.
Leisinger
T.
(
1983
)
Oxygen sensitivity of methanogenic bacteria
Syst. Appl. Microbiol.
 ,
4
,
313
325
.
[12]
Schlichting
E.
Blume
H.P.
(
1966
)
Bodenkundliches Praktikum
 
Parey
Hamburg
.
[13]
Remde
A.
Slemr
F.
Conrad
R.
(
1989
)
Microbial production and uptake of nitric oxide in soil
FEMS Microbiol. Ecol.
 ,
62
,
221
230
.
[14]
Widdel
F.
Pfennig
N.
(
1981
)
Studies on dissimilatory sulfate-reducing bacteria enriched fatty acids. I. Isolation of new sulfate-reducing bacteria enriched with acetate from saline environements. Description of Desulfobacter postgatei gen.nov.sp.nov
.
Arch. Microbiol.
 ,
129
,
395
400
.
[15]
Siebert
M.L.
Hattingh
W.H.J.
(
1967
)
Estimation of methane producing bacterial numbers by the most probable number (MPN) technique
Water Res.
 ,
1
,
13
19
.
[16]
Conrad
R.
Bak
F.
Seitz
H.J.
Thebrath
B.
Mayer
H.P.
Schütz
H.
(
1989
)
Hydrogen turnover by psychotrophic homoacetogenic and mesophilic methanogenic bacteria in anoxic paddy soil and lake sediment
FEMS Microbiol. Ecol.
 ,
62
,
285
294
.
[17]
Bartlett
R.J.
(
1986
)
Soil redox behaviour
and
Sparks
D.L.
, Ed) pp.
Soil Physical Chemistry
 
179
207
CRC Press
,
Boca Raton, FL
.
[18]
Stumm
W.
(
1967
)
Redox potentials as an environmental parameter
and
Jaag
O.
, Ed)
3. Aufl.
In Advaces in Water Pollution Research, In
Conceptual significance and operational limitation
 , pp.
283
308
Pergamon Press
,
New York
.
[19]
Conrad
R.
Schütz
H.
Babbel
M.
(
1987
)
Temperature limitation of hydrogen turnover and methanogenesis in anoxic paddy soil
FEMS Microbiol. Ecol.
 ,
45
,
281
289
.
[20]
Conrad
R.
Babbel
M.
(
1989
)
Effect of dilution on methanognesis, hydrogen turnover and interspecies hydrogen transfer in anoxic paddy soil
FEMS Microbiol. Ecol.
 ,
62
,
21
27
.
[21]
Conrad
R.
Mayer
H.P.
Wüst
M.
(
1989
)
Temporal change of gas metabolism by hydrogen-syntrophic methanogenic bacterial associations in anoxic paddy soil
FEMS Microbiol. Ecol.
 ,
62
,
265
274
.
[22]
Jacobsen
P.
Patrick
W.H.
Jr.
Williams
B.G.
(
1981
)
Sulfide and methane formation in soils and sediments
Soil Sci.
 ,
132
,
279
287
.
[23]
Schütz
H.
Seiler
W.
Conrad
R.
(
1989
)
Processes involved in formation and emission of methane in rice paddies
Biogeochemistry
 ,
7
,
33
53
.
[24]
Giani
D.
Giani
L.
Cohen
Y.
Krumbein
W.E.
(
1984
)
Methanogenesis in the hypersaline Solar Lake (Sinai)
FEMS Microbiol. Lett.
 ,
25
,
219
224
.
[25]
Patel
G.B.
Roth
L.A.
Agnew
B.J.
(
1984
)
Death rates of obligate anaerobes exposed to oxygen and the effect of media prereduction on cell viability
Can. J. Microbiol.
 ,
30
,
228
235
.
[26]
Cypionka
H.
Widdel
F.
Pfennig
N.
(
1985
)
Survival of sulfate-reducing bacteria after oxygen stress, and growth in sulfate-free oxygen-sulfide gradients
FEMS Microbiol. Ecol.
 ,
31
,
39
45
.
[27]
Gottschal
J.C.
Szewyzk
R.
(
1985
)
Growth of a facultative anaerobe under oxygen-limiting conditions in pure culture and in co-culture with a sulfate-reducing bacterium
FEMS Microbiol. Ecol.
 ,
31
,
159
170
.
[28]
Tiedje
J.M.
(
1988
)
Ecology of denitrification and dissimilatory nitrate reduction to ammonium
and
Zehnder
A.J.B.
, Ed) pp.
Biology of Anaerobic Microorganisms
 
179
244
Wiley
,
New York
.