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Kathrin Reiber, Emer P. Reeves, Claire M. Neville, Robert Winkler, Peter Gebhardt, Kevin Kavanagh, Sean Doyle, The expression of selected non-ribosomal peptide synthetases inAspergillus fumigatus is controlled by the availability of free iron, FEMS Microbiology Letters, Volume 248, Issue 1, July 2005, Pages 83–91, https://doi.org/10.1016/j.femsle.2005.05.028
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Abstract
Three non-ribosomal peptide synthetase genes, termedsidD, sidC andsidE, have been identified inAspergillus fumigatus. Gene expression analysis by RT-PCR confirms that expression of bothsidD andC was reduced by up to 90% under iron-replete conditions indicative of a likely role in siderophore biosynthesis.SidE expression was less sensitive to iron levels. In addition, two proteins purified from mycelia grown under iron-limiting conditions corresponded to SidD (200 kDa) and SidC (496 kDa) as determined by MALDI ToF peptide mass fingerprinting and MALDI LIFT-ToF/ToF. Siderophore synthetases are unique in bacteria and fungi and represent an attractive target for antimicrobial chemotherapy.