Ciprofloxacin binding to GyrA causes global changes in the proteome of Pseudomonas aeruginosa

Abstract Ciprofloxacin is one of the most widely-used antibiotics, and has proven especially effective at controlling infections associated with the opportunistic human pathogen, Pseudomonas aeruginosa. In this work, we show that sub-inhibitory concentrations of ciprofloxacin induce discrete changes in the intracellular proteome. Central metabolism and cell envelope-associated functions are particularly affected. In spite of the low magnitude of the intracellular proteomic changes, we found that sub-lethal concentrations of ciprofloxacin had substantial effects on motility and exoprotein secretion. Crucially, the proteomic and phenotypic modulations that we observed were absolutely dependent upon the presence of wild-type GyrA; an isogenic strain of P. aeruginosa carrying a ciprofloxacin-insensitive form of GyrA (a T83→I mutant) did not display ciprofloxacin-dependent changes unless complemented with wild-type gyrA in trans. These results show that the diverse effects of sub-inhibitory ciprofloxacin on the cell are routed through its primary target in the cell, DNA gyrase.


INTRODUCTION
Pseudomonas aeruginosa is an opportunistic pathogen responsible for causing a variety of human disease states, especially among immunocompromised individuals. Indeed, in 2017, the World Health Organisation (WHO) named P. aeruginosa as a critical priority pathogen against which new antimicrobial interventions are urgently required. One of the most effective antibiotics used to treat P. aeruginosa infections is the second generation fluoroquinolone, ciprofloxacin (CIP) (Wise, Andrews and Edwards 1983). Introduced in 1987, CIP proved so effective at treating a wide range of bacterial infections that it rapidly joined the WHO list of medicines essential for basic healthcare. CIP has a broad spectrum of action with good tissue penetration, oral absorption and favourable phamacokinetics, making it ideal for the treat-ment of a wide range of infections. Crucially, the presence of the cyclopropane moiety on the N atom of the heterocycle in CIP increases its activity (compared with first generation fluoroquinolones such as norfloxacin) against P. aeruginosa by a factor of four.
In Gram-negative bacteria, the primary target of CIP is DNA gyrase (a tetramer comprised of 2 GyrA and 2 GyrB molecules), although topoisomerase-IV (ParC) is a secondary target and can be inhibited by higher concentrations of the drug. Quinolones do not bind to the gyrase enzyme alone, but rather, to the gyrase-DNA complex (Hooper 2001). The region of GyrA spanning residues 51-106 is known as the quinoloneresistance determining region (QRDR), since mutations within this sequence decrease the susceptibility of the organism to fluoroquinolone inhibition (Yoshida et al. 1990). Not surprisingly, this stretch of sequence maps to the recognition helix responsible for binding DNA. Among QRDR mutants, substitutions at residue 83 are frequently associated with CIP resistance in both P. aeruginosa and Escherichia coli clinical isolates (reviewed by Hooper 1999). In many CIP-resistant gyrA mutants, residue 83 is replaced by a bulkier, non-polar residue that is thought to impede DNA-binding to gyrase, and hence, also fluoroquinolone binding. Interestingly in E. coli, residue 83 is a serine, whereas in P. aeruginosa it is the bulkier threonine; this may correlate with the higher intrinsic resistance of P. aeruginosa to CIP compared with E. coli (Fabrega et al. 2009).
Previous transcriptomic analyses have revealed that exposure of P. aeruginosa cultures to sub-minimal inhibitory concentrations of CIP (sub-MIC CIP ) brings about large-scale changes in gene expression, with hundreds of transcripts showing altered abundance in CIP-treated cultures (Brazas and Hancock 2005). This raises the question of whether these modulations are a direct consequence of GyrA inhibition, or whether CIP also has 'off target' effects. To address this, in the current work, we used quantitative 2D-difference gel electrophoresis (2D-DiGE) to compare the adaptive response of wild-type P. aeruginosa and an isogenic T83→I gyrA mutant to sub-MIC CIP . Our results suggest that sub-MIC CIP has significant impacts on central metabolism and protein secretion, and these effects are largely dependent upon the presence of a CIP-sensitive gyrA allele. Very few off-target effects are apparent, even at very high concentrations of CIP.

Bacterial strains used
The PAO1 employed in the current study was a generous gift from Barbara Iglewski (University of Rochester, NY). HGS4 is a derivative of that PAO1 strain.

Cloning of gyrA for complementation
A 3 kb DNA fragment including the gyrA coding region and its associated Shine-Dalgarno sequence was PCR-amplified (LT Polymerase, and GyrApR (5'-AGTCAACCCGGGAGAAATTGAAGGCTCGCTTG-3')). The fragment was sequentially digested with SmaI/SacI and ligated with pUCP20 (West et al. 1994) that had been digested with the same enzymes. This yielded pGyrA.

Media
Unless otherwise stated, all cultures were grown in AGSY medium (Stickland et al. 2010).

Minimum inhibitory concentration determination
Minimum inhibitory concentrations (MICs) were determined using E-Test strips laid onto a lawn of the relevant strain grown on the surface of AGSY-agar plates.

Whole cell 2D-DiGE Proteomic Analysis
2D-DiGE Biological Variance Analysis (BVA; GE Healthcare) was used to analyse the proteomic changes accompanying treatment of wild-type P. aeruginosa and the isogenic T83→I gyrA mutant to sub-MIC CIP . Four independent biological replicates of wild-type PAO1 (each treated with 0, 0.05, 0.075 μg/ml CIP) and HGS4 (0, 0.075, 0.25 μg/ml CIP) cultures were analysed. When present, sub-MIC CIP was added at the start of each growth curve. The cultures were grown in AGSY medium with good aeration, as previously described (Stickland et al. 2010) and harvested for proteomic analysis when they reached early stationary phase (OD 600 = 6, ca. 5-6 hrs after inoculation). The 2D-DiGE, spot identification and statistical analyses were carried out as previously described (Stickland et al. 2010).

Secretome protein extraction
Protein from the secretome of PAO1 (pUCP20), HGS4 (pUCP20) and HGS4 (pGyrA) cultures grown with or without 0.075 μg/ml CIP was analysed. Extracellular material was collected at OD 600 = 6 from cultures grown in AGSY supplemented with 50 μg/ml carbenicillin (to maintain the introduced pUCP20 or pUCP20-derived plasmids). Secreted proteins were precipitated from filter-sterilised culture supernatant by addition of 12.5% (w/v, final concentration) trichloroacetic acid (TCA), essentially as described previously (Swatton et al. 2016). The mixture was left on ice overnight and precipitated proteins were collected by centrifugation (12,000 × g, 4 • C). The pellet was washed three times in 80% acetone, air-dried, and then resuspended in 50mM Tris-HCl (pH 6.8) containing 10% glycerol and 2% SDS. The pH was adjusted to ∼ pH 7 and the protein concentration was determined (BioRad detergent-compatible quantification kit). Proteins were resolved by SDS-PAGE (10-20% gradient gels).

Swarming
For swarming motility assays, 5 μl of an overnight culture diluted to an OD of 0.1 was carefully pipetted onto the surface of 0.7% (w/v) Eiken agar prepared in AGSY medium and incubated overnight. The plates were incubated at 30 • C so that motility zones were not too large to measure. Swarming motility was qualitatively assessed by how far the cells had travelled from the point of inoculation and for tendril formation.

Isolation of a spontaneous ciprofloxacin-resistant gyrA mutant
Wild-type PAO1 (MIC CIP = 0.25 μg/ml in AGSY medium) was inoculated into AGSY medium containing 1 μg/ml CIP and incubated with good aeration at 37 • C. After 24 hours, the culture had become cloudy, indicating the emergence of spontaneous CIP-resistant mutant(s). Samples from the 24 hours culture were serially-diluted and spread onto agar plates containing 1 μg/ml CIP to yield single colonies. A range of colony morphotypes were observed. Targeted PCR/sequence analysis revealed that some of these colonies contained nfxB mutations (which lead to upregulation of the normally cryptic mexCD-oprJ CIP efflux pump (Poole et al. 1996;Jeannot et al. 2008)), whereas others were wildtype for nfxB. These results indicate that even within a single overnight culture, CIP-resistance can arise due to different mutations. A single CIP-resistant colony that did not contain an nfxB mutation was picked for further analysis. Targeted PCR amplification and sequencing of the QRDR of gyrA revealed that the amplicon contained a C→T transition converting the Thr83 codon (ACC) in the gyrA ORF to an Ile codon (ATC). The T83→I alteration is a well-established cause of CIP-resistance (Hooper 1999;Hooper 2001). This gyrA mutant was named HGS4. The QRDR of the parC locus was also amplified and sequenced to confirm that no parC mutations were present in HGS4. To further demonstrate that the increased CIP-resistance of HGS4 was solely due to the gyrA mutation, the gyrA ORF and its associated Shine-Dalgarno sequence was PCR-amplified from wild-type genomic DNA and cloned into pUCP20, generating pGyrA. The MIC CIP of pGyrAcomplemented HGS4 was restored to near wild-type levels (ca. 0.5 μg/mL), whereas the MIC CIP of HGS4 containing pUCP20 remained high (MIC CIP = 4-6 μg/ml (Fig. 1A).

Proteomic adaptations of P. aeruginosa to ciprofloxacin
CIP concentrations of ≤ 0.075 μg/ml (corresponding to 0.3 × MIC wild-type ) did not affect the growth of PAO1 in AGSY, although concentrations greater than this led to a reduction in growth rate and stationary phase optical density. In contrast, the gyrA mutant (HGS4) tolerated concentrations of 0.25 μg/ml CIP (1 × the wild-type MIC CIP ) without any apparent effect(s) on growth (Fig. 1B). For proteomic analysis, we therefore challenged wild-type PAO1 cultures with 0.05 μg/ml CIP and 0.075 μg/ml CIP (0.2 × MIC and 0.3 × MIC, respectively), whereas the HGS4 cultures were challenged with 0.075 μg/ml CIP and 0.25 μg/ml CIP. These concentrations were selected for study (i) to allow comparison of the proteomic changes brought about at the highest non-growth rate impairing concentration of CIP in the wild-type (0.075 μg/ml) with the target site mutant (HGS4), and, (ii) to examine whether a lethal concentration (for the wild-type strain) of CIP elicits any proteomic changes in the target site mutant (0.25 μg/ml CIP is equal to the MIC of the wild-type strain). Untreated cultures served as controls. Whole cell lysates were analyzed by 2D-difference gel electrophoresis (2D-DiGE) using a BVA protocol, allowing smaller fold-changes to be reliably determined (Stickland et al. 2010). This work was carried out as part of a larger BVA, which also included comparison of the wild-type with an nfxB mutant. Mutations in nfxB confer resistance to CIP by up-regulating expression of the MexCD-OprJ efflux pump (Shiba et al. 1995;Poole et al. 1996). Our findings assessing the impact of nfxB mutation on the proteome have been previously published (Stickland et al. 2010) and the master gel for the BVA was presented in that study. Until now, the outcome of the second half of that BVA study (examining the impact of CIP on the wild-type proteome) has not been reported.
The average ratio of standardized protein spot abundance was calculated for each of the spots in the filtered dataset. To our surprise, even the highest concentration of CIP tested had little effect on the global proteome; only 24 protein spots (out of a total of 853 spots) were significantly (P ≤ 0.01) modulated in the CIP-treated wild-type samples compared with the untreated controls (Fig. 2, upper panel). Interestingly, not all of the proteins that were modulated at the lower concentration of CIP (0.05 μg/ml) were also modulated at the higher concentration (0.075 μg/ml) of CIP (although there was some overlap). This may indicate that the cell implements a 'graded' response to the antibiotic, possibly reflecting the impact of differential gyrase inhibition on gene expression profiles (DNA gyrase-dependent supercoiling is known to influence transcription (reviewed by Dorman and Dorman 2016)) or differential stimulation of reactive oxygen species production (see Discussion). The proteome of untreated HGS4 was essentially indistinguishable from that of the untreated wild-type (data not shown). This indicates that the T83→I substitution in HGS4 GyrA does not in itself lead to obvious cellular perturbations. Also, fewer and smaller changes were elicited by addition of CIP to HGS4 cultures (Fig. 2, lower panel). None of the spots modulated by CIP in HGS4 overlapped with the CIP-modulated spots in the wild-type. These data suggest that in the wild-type, most of the observed changes are dependent upon the interaction of CIP with GyrA.

Multivariate analysis of the proteomic data
Our finding, that only small numbers of protein spots were significantly modulated by sub-MIC CIP exposure, contrasts strongly with earlier microarray experiments which reported that hundreds of transcripts are modulated by sub-MIC CIP (Brazas and Hancock 2005). One possible explanation for this disparity is that many of the proteomic changes elicited by CIP treatment are subtle and escape detection based on univariate statistical analyses, which only assess pairwise comparisons. We therefore re-analysed the proteomic dataset using an unsupervised multivariate approach, principal components analysis (PCA). A scores plot of the first two principal components in the model (Fig. 3A) shows that the CIP-treated wild-type samples show a trend towards segregating away from the untreated samples, with the higher CIP concentration driving greater separation. To further tease out the drivers underpinning these separations, a more sensitive, supervised approach (partial least squaresdiscriminant analysis, PLS-DA) was employed. The first component accounted for the majority (83%) of the differences between the sample types (data not shown). To establish how many protein spots contributed towards this separation, we iteratively removed spots from the PLS-DA model until the robustness (Q 2 value) of the model collapsed (Fig. 3B) (Karp, Griffin and Lilley 2005). This happened after ∼200 ± 25 protein spots were removed, indicating that around 23% (200/853 spots) of the observed proteome was modulated by sub-MIC CIP treatment. Our data are therefore broadly consistent with earlier microarraybased approaches indicating that CIP-induced alterations involve hundreds of gene products. Many of these modulations were subtle and involved low-fold changes, consistent with maintenance of a relatively constant intracellular proteomic steady state. Figure 2. Numbers of protein spots modulated (P ≤ 0.01) in the cell-associated proteome of (A) the wild-type or (B) HGS4 following exposure to the indicated concentration of CIP (all modulations relative to the untreated wild-type or HGS4 proteome, respectively). The tables show whether the spots were up-or downregulated. For example, of the 12 spots that were modulated by 0.05 μg/mL CIP in the wild-type, 3 showed decreased abundance in the treated samples (cf. the untreated samples) and 9 showed increased abundance in the treated samples. In an iterative process involving removal of the top 50 spots, followed by recalculation of the model, we found that the model collapsed (i.e. lost predictive power) after removal of 200 ± 25 spots. This indicates that sub-MICCIP treatment of wild-type P. aeruginosa alters the abundance of two hundred protein spots.

Identities of the modulated spots
A selection of the CIP-modulated spots identified in both the multivariate and t-test analyses were excised from the gels and tryptically digested to yield peptide fragments. These peptides were then identified by LC-MS/MS analysis and MASCOT searching. Spots which yielded identities for two or more proteins (i.e. 'mixture hits' arising from overlapping spots) were excluded from further analysis. The identified proteins in the CIP-modulated spots from the wild-type and HGS4 samples are shown in Table 1 and Table S1, respectively. There was no overlap between the identified proteins in the wild-type and HGS4 samples. The modulated spots were classified into functional groups according to their PseudoCAP ontology (www.pseudomonas.com/). Notably, CIP treatment resulted in the modulation of several enzymes catalyzing reactions that are either part of the TCA cycle or that feed in/out of the TCA cycle. These include pyruvate kinase (PykA), the superoxide-insensitive isozyme of fumarate hydratase (FumC1), two differentially modulated isozymes of isocitrate dehydrogenase (PA2623 (icd, down-regulated) and PA2624 (idh, up-regulated)) and malic enzyme. Consistent with the up-regulation of FumC1 (indicating oxidative stress), we also observed that the house-keeping catalase (KatA) was . T-tests were carried out comparing untreated wild-type samples with wild-type samples that had been treated with the indicated concentration of CIP. The magnitude and direction of modulation is shown as fold-change (for proteins identified by t-test) and by the arrows. A protein identification was considered significant if the MASCOT score was > 106 (the equivalent of two different significant peptide ion matches). * Indicates that a protein was identified in more than one spot.
up-regulated. Other notable modulated proteins included the chaperonin, GroEL (multiple spots up-regulated), the flagellar capping protein, FliD, proteins involved in cell envelope maintenance such as MurE (UDP-MurNac-tripeptide synthetase; up-regulated) and MreB (a DnaK-like molecular chaperone involved in determining cell shape; down-regulated) and a DegP/HtrA-like serine protease (MucD) involved in the control of alginate production and response of envelope stress (down-regulated).
Of the identified proteins modulated in HGS4, most were down-regulated, and most of these were affected only at the higher concentration of CIP tested (0.25 μg/ml), perhaps indicative of off-target or secondary target effects of CIP. Most notably, GyrB was down-regulated, as was the AAA + ATPase family chaperone, ClpB.

Phenotypic consequences of CIP-challenge
To investigate the possible consequences of the proteomic modulations, we examined a selection of phenotypes that might be affected by these. Motility plays a key role in infection, and our proteomic analysis showed that the flagellar capping protein, Figure 4. Ciprofloxacin depresses tendril formation during surface swarming by inhibiting GyrA. Aliquots of culture containing the indicated strains were spotted onto 0.7% (w/v) Eiken agar plates and left at 30 • C to allow swarm development. (i) WT (pUCP20), (ii) HGS4 (pUCP20) and (iii) HGS4 (pGyrA). Note that CIP depresses tendril formation in the wild-type but not in the gyrA T83→I mutant, HGS4. However, tendril formation is depressed in HGS4 following expression of wild-type gyrA in trans.
FliD, was down-regulated in the presence of CIP. Consistent with this, we noted that the surface swarming ability of wild-type P. aeruginosa was impaired in the presence of 0.075 μg/ml CIP (Fig. 4). No impairment of motility was seen in the CIP-treated HGS4 mutant. However, when HGS4 was complemented with pGyrA, surface swarming once again became sensitive to the presence of CIP. We conclude that the effect of CIP on motility is dependent on the presence of a susceptible gyrA allele in the wild-type.
The altered motility elicited by CIP, as well as the identification of several CIP-modulated proteins associated with cell envelope physiology made us wonder whether protein secretion might be affected by the drug. Indeed, earlier workers have shown that CIP treatment of P. aeruginosa biofilms alters the spectrum of proteases secreted (O ldak and Trafny 2005). Therefore, we used 1D SDS-PAGE to analyse the secretome of wildtype PAO1 (pUCP20), HGS4 (pUCP20) and HGS4 (pGyrA). Sub-MIC CIP caused clear alterations in the profile of proteins secreted by cultures of wild-type PAO1, but no obvious major alterations in the protein profile secreted by HGS4 (Fig. 5, upper panel). However, when HGS4 was complemented with pGyrA, the secretome once again became sensitive to CIP addition. These data suggest that sub-MIC CIP treatment impacts upon protein secretion by P. aeruginosa through its effects on GyrA. To confirm that the observed secretome alterations were not due to increased CIP-dependent cell lysis, we used Western blotting to assess β-lactamase levels in the secretome samples. β-lactamase is constitutively expressed from the pUCP20 plasmids in each strain and is a periplasmic enzyme. Therefore, release of β-lactamase is a good indicator of cell envelope integrity. There was no evidence of enhanced cell lysis in the CIP-treated samples (Fig. 5, lower panel).

DISCUSSION
In this study, we show that the adaptive response to sub-MIC CIP involves a small number of moderate (<2-fold) changes in intracellular protein abundance, and a much larger number of lower-fold modulations. These subtle modulations are consistent with maintenance of a relatively constant overall intracellular steady state proteome. Larger CIP-induced proteomic modulations were seen in the exoproteome, which is presumably subject to less stringent control of its composition. However, we also show that in spite of the low magnitude of the intracellu- lar proteomic modulations, some of these have clear phenotypic consequences. We further show that these sub-MIC CIP -induced phenotypic and proteomic changes are absolutely dependent upon the drug interacting with wild-type (CIP-sensitive) GyrA. Indeed, even at a concentration of CIP that completely prevents the growth of wild-type cultures (i.e. 0.25 μg/ml, equal to the MIC CIP of PAO1), the gyrA T83I mutant showed very few proteomic changes.
In a previous study, Brazas et al used microarray analysis to investigate the effects of sub-MIC CIP on the global gene expression profile of P. aeruginosa (Brazas and Hancock 2005). Those workers found that the transcripts from 941 genes were affected in the presence of 0.3 × MIC CIP . In contrast, we observed that only around 200 protein spots were affected by 0.3 × MIC CIP . However, this may simply reflect differences in the relative sampling efficiency (i.e. gene products detectable) of proteomics compared with transcriptomics. Moreover, 60% of the gene products reported in the current study were not reported as being modulated by Brazas et al. and of the gene products that were common to both studies, only four (GroEL, Icd, IlvE and PA4595) were modulated in the same direction. It seems likely that the main driver for the disparity between the two studies lies in biological differences, probably related to the different growth conditions employed (Brazas et al employed oligopeptide-based LB for their study, while we used alanine-glycerol-salts medium).
Why should inhibition of DNA gyrase lead to pleiotropic changes in the cell? Davies and colleagues have shown that the cellular response to many antibiotics exhibits the property of hormesis, suggesting that these compounds may have evolved as intercellular signals (Davies, Spiegelman and Yim 2006). A variation on this scenario is that low concentrations of antibiotic may induce protective measures that 'prime' the cell ready to respond higher concentrations of the same compound. While this may indeed be the case, we note that CIP is an entirely synthetic antibiotic, so there is no reason to believe that the cell will have evolved mechanisms to respond specifically to its presence. However, just as the ribosome can titrate translational inhibitors, we suspect that DNA gyrase may monitor the general 'state of health' of the genome and allow the cell to adapt accordingly. Two possible generic gyrase-dependent adaptatory mechanisms present themselves. First, the observed proteomic modulations may arise as a consequence of altered gene expression brought about directly by GyrA inhibition. Indeed, DNA gyrase-dependent supercoiling is known to affect the expression of many genes (Dorman and Dorman 2016). In this scenario, adaptations which protect against the effects of GyrA inhibition would be hardwired into the global gyrase regulon. The second mechanism is indirect and involves adaptive measures that protect against the bactericidal effect(s) of the antibiotic. Kohanski et al. (2007) have proposed that all bactericidal antibiotics (including CIP) exhibit a common mechanism of killing. In this model, inhibition of the target molecule activity leads, via transient stimulation of superoxide production, to a runaway Fenton reaction and subsequent accumulation of lethal hydroxyl radicals. In this case, the proteomic changes induced by exposure to low concentrations of the antibiotic would be expected to comprise part of the cell's adaptive response to the imposed (antibiotic-dependent) oxidative stress. While we cannot distinguish between these two possible modes of action, it is clear from Table 1 that enzymes involved in the response to oxidative stress (KatA, FumC1) are indeed up-regulated by exposure to sub-MIC CIP .
Another set of potentially related gene products that were modulated by sub-MIC CIP are involved in metabolism. Malic enzyme (PA5046), which catalyses the oxidative decarboxylation of malate to yield pyruvate, was down-regulated, whereas pyruvate kinase (PykA) was up-regulated. This suggests that a net effect of CIP exposure is to decrease the flow of metabolites out of the TCA cycle (e.g. for gluconeogenesis) and increase the flux of carbon going into the cycle. In addition, the isocitrate dehydrogenase encoded by icd was down-regulated whereas the NADP-dependent isocitrate dehydrogenase isozyme encoded by idh was up-regulated. Little is known about these two isozymes in P. aeruginosa, although it seems likely that they are differentially regulated. Crucially, the products of the isocitrate dehydrogenase reaction are α-ketoglutarate and NADPH, both of which play an important role in the response to oxidative stress (Mailloux et al. 2007;Spaans et al. 2015). Taken together, it seems likely that exposure to sub-MIC CIP leads to an accumulation of reactive oxygen species (as per the Kohanski model), and that in response to this, the cell increases flux around the TCA cycle to generate α-ketoglutarate and NADPH. Consistent with this, we further note that the cystathionine γ -lyase, MetB, was also up-regulated upon exposure to CIP. Cystathionine γ -lyase breaks down cystathionine to yield α-ketoglutarate.
Interestingly, one of the gene products (PA4595) identified in both this and a previous transcriptomic study (Brazas and Hancock 2005) as being down-regulated in the presence of sub-MIC CIP is a predicted ATP-binding component of an ABC transporter. The PA4595 gene is located immediately adjacent to a cell envelope stress-inducible nfxB-like repressor, esrC. EsrC and nfxB flank the mexCD-oprJ multidrug efflux pump, well-known for being a major determinant of CIP resistance (Stickland et al. 2010), and both regulators are known to affect expression of the pump under different conditions (Purssell et al. 2015). The functional relationship, if any, between PA4595 and EsrC/MexCD-OprJ/NfxB is not clear, and the location of PA4595 adjacent to this known CIP resistance determinant may be entirely coincidental. However, we previously showed that the protein encoded by PA4595 is up-regulated in an nfxB mutant (Stickland et al. 2010), suggesting a possible functional linkage.
In summary, we have shown that exposure to sub-MIC CIP leads to re-modelling of the intracellular proteome. This remodeling has phenotypic consequences, and is dependent on the binding of CIP to a sensitive GyrA protein. Many of the proteomic changes that are elicited by sub-MIC CIP appear to be linked with the cellular response to oxidative stress, although we cannot exclude the possibility that some of these modulations reflect an altered DNA supercoiling state in the cell as the DNA gyrase becomes progressively more inhibited by higher concentrations of CIP.