Abstract

Siderophore produced by cowpea Rhizobium GN1 (Peanut isolate) was shown to be involved in iron uptake by this organism. Siderophore enhanced iron uptake in iron-starved cells. SDS-PAGE analysis of the outer membrane proteins showed two iron repressible outer membrane proteins with approximate molecular mass of 80 kDa and 76 kDa. A siderophore non-producing mutant, which was unable to grow on a medium containing synthetic iron chelators unless and until iron was added exogenously in the medium, could use siderophore of the wild-type for iron uptake indicating that the receptor for Fe-siderophore complex was intact in the mutant.

References

1
Byers
R.
Arceneaux
J.L.
(
1977
)
Microbial transport and utilization of iron
2nd ed.
(
Weinberg
E.D.
, Ed) Vol.
3
, In
Microorganisms and Minerals
 , pp
215
249
Marcel Dekker Inc
,
New York
.
2
Smith
M.J.
Shoolery
J.N.
Schwyn
B.
Holden
I.
Neilands
J.B.
(
1985
)
Rhizobactin, a structurally novel siderophore from Rhizobium meliloti
J. Am. Chem. Soc.
 ,
107
,
1739
1743
.
3
Carson
K.C.
Holliday
S.
Glenn
A.R.
Dilworth
M.J.
(
1992
)
Siderophore and organic acid production in root nodule bacteria
Arch. Microbiol.
 ,
157
,
264
271
.
4
Jadhav
R.S.
Desai
A.J.
(
1992
)
Isolation and characterization of siderophore from cowpea Rhizobium (peanut isolate)
Curr. Microbiol.
 ,
24
,
537
542
.
5
Guerinot
M.L.
Meidl
E.L.
Plessner
O.
(
1990
)
Citrate as a siderophore in Bradyrhizobium japonicum
J. Bacteriol.
 ,
172
,
3298
3303
.
6
Raymond
K.N.
Muller
G.
Matzanke
B.F.
(
1984
)
Complexation of iron by siderophores: A review of their solution and structural chemistry and biological function
Top. Curr. Chem.
 ,
123
,
49
102
.
7
Neilands
J.B.
(
1982
)
Microbial envelope proteins related to iron
Annu. Rev. Microbiol.
 ,
32
,
285
309
.
8
Meyer
J.M.
Hohnadel
D.
Khan
A.
Cornelis
P.
(
1990
)
Pyoverdin-facilitated iron uptake in Pseudomonas aeruginosa: Immunological characterization of the ferripyoverdin receptor
Mol. Microbiol.
 ,
4
,
1401
1405
.
9
McIntosh
M.A.
Earhart
C.F.
(
1977
)
Coordinate regulation by iron of the synthesis of phenolate compounds and three outer membrane proteins in Escherichia coli
J. Bacteriol.
 ,
131
,
331
339
.
10
Arnow
L.E.
(
1937
)
Colorimetric determination of the components of 3,4-dihydroxyphenylalanine-tyrosine mixtures
J. Biol. Chem.
 ,
118
,
314
535
.
11
Page
W.J.
Huyer
M.
(
1984
)
Derepression of the Azotobacter vinelandii siderophore system using iron containing minerals to limit iron repletion
J. Bacteriol.
 ,
158
,
496
502
.
12
Lugtenberg
B.J.
Meijers
R.
Peters
P.
Van der Hoek
A.
Van Alphen
L.
(
1975
)
Electrophoretic resolution of the outer membrane proteins of E. coli K-12 into 4 bands
FEBS Lett.
 ,
58
,
254
258
.
13
Arceneaux
J.L.
Lankford
C.E.
(
1966
)
A schizokinen (siderochrome) auxotroph of Bacillus megaterium induced with N-methyl-N′-nitro-N-nitrosoguanidine
Biochem. Biophys. Res. Commun.
 ,
24
,
370
375
.
14
Neilands
J.B.
Schwyn
B.
(
1987
)
Universal chemical assay for the detection and determination of siderophores
Anal Biochem.
 ,
160
,
47
56
.
15
Lammers
P.J.
Sanders-Loehr
J.
(
1982
)
Active transport of ferric schizokinen in Anabaena sp.
J. Bacteriol.
 ,
151
,
288
294
.
16
Knosp
O.
von Tigerstrom
M.
Page
W.J.
(
1984
)
Siderophore mediated iron uptake in Azotobacter vinelandii
J. Bacteriol.
 ,
159
,
341
347
.
17
Marugg
J.D.
Van Spange
M.
Hoekstra
W.P.M.
Schippers
B.
Weisbeek
P.J.
(
1985
)
Isolation and analysis of genes involved in siderophore biosynthesis in plant growth stimulating Pseudomonas putida WCS358
J. Bacteriol.
 ,
164
,
563
570
.
18
Glick
B.R.
Menhart
N.
Soong
N.W.
Farmer
W.J.
Vishwanatha
T.
Pasternak
J.J.
(
1988
)
Isolation and partial characterization of siderophore mutants of Azotobacter vinelandii
Curr. Microbiol.
 ,
17
,
343
346
.