Abstract

Genomic DNA from 15 strains of Helicobacter mustelae was subjected to pulsed-field gel electrophoresis (PFGE) after digestion with PacI and SfiI. H. mustelae genome DNA appeared very similar in all strains examined, whether isolated from ferrets or mink or from animals bred in either the USA or in the UK. The H. mustelae genome size was estimated to be 1.7 Mb, similar in size to that of H. pylori. A minor difference in PacI PFGE pattern and genome size was observed between rifampicin-resistant and rifampicin-susceptible derivatives of H. mustelae F251. Another minor difference in genome pattern based on PFGE with SfiI was observed between an H. mustelae strain used to experimentally infect four ferrets which resulted in loss of an SfiI site in strains obtained from the newly infected ferrets. Thus, although minor differences in PFGE pattern were noted, H. mustelae lacks the genomic diversity observed in H. pylori.

References

1
Akopyanz
N.
Bukanov
N.O.
Westblom
T.U.
Kresovich
S.
Berg
D.E.
(
1992
)
PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori
Nucleic Acids Res.
 ,
20
,
5137
5142
.
2
Blaser
M.J.
(
1987
)
Gastric Campylobacter-like organisms, gastritis and peptic ulcer disease
Gastroenterology
 ,
93
,
371
383
.
3
Chang
N.
Taylor
D.E.
(
1990
)
Use of pulsed-field gel electrophoresis to size genomes of Campylobacter species and to construct a SalI map of Campylobacter jejuni UA580
J. Bacteriol.
 ,
172
,
5211
5217
.
4
Correa
P.
(
1992
)
Human gastric carcinogenesis: a multistep and multifactorial process
First American Career Society Award Lecture on Cancer Epidemiology and Prevention Cancer Research
 ,
52
,
6735
6740
.
5
Dick
J.D.
(
1990
)
Helicobacter (Campylobacter) pylori: a new twist to an old disease
Ann. Rev. Microbiol.
 ,
108
,
70
90
.
6
Fox
J.G.
Blanco
M.
Polidoro
D.
Yan
L.-L.
Dewhirst
F.E.
Paster
B.J.
(
1993
)
Role of gastric pH in isolation of H. mustelae from the feces of ferrets
Gastroenterology
 ,
104
,
86
92
.
7
Fox
J.G.
Cabot
E.B.
Taylor
N.S.
Laraway
R.
(
1988
)
Gastric colonization by Campylobacter pylori subsp. mustelae in ferrets
Infect. Immun.
 ,
45
,
2994
2996
.
8
Fox
J.G.
Correa
P.
Taylor
N.S.
Lee
A.
Otto
G.
Murphy
J.C.
Rose
R.
(
1990
)
Helicobacter mustelae-associated gastritis in ferrets
Gastroenterology
 ,
99
,
352
361
.
9
Fox
J.G.
DeCross
A.
Taylor
N.S.
Yan
L.-L.
Dewhirst
F.E.
Paster
B.J.
Meade
L.
Krakowka
S.
Gorham
J.
Marshall
B.
(
1993
)
Initial isolation and characterization of Helicobacter mustelae from the stomach of mink (Mustelae vison)
Acta Gastro-Enterol. Belgia
 ,
56
,
97
(Suppl.).
10
Fox
J.G.
Ho
G.
Taylor
N.S.
Rosenblad
W.
Murphy
J.C.
(
1991
)
Helicobacter mustelae-induced gastritis and elevated gastric pH in the ferret (Mustela putorius furo)
Infect. Immun.
 ,
59
,
1875
1880
.
11
Fox
J.G.
Paster
B.J.
Dewhirst
F.E.
Taylor
N.S.
Yan
L.-L.
Macuch
P.J.
Chmura
L.M.
(
1992
)
Helicobacter mustelae isolation from feces of ferrets: evidence to support fecal-oral transmission of a gastric Helicobacter
Infect. Immun.
 ,
60
,
606
611
.
12
Fox
J.G.
Taylor
N.S.
Edmonds
P.
Brenner
D.J.
(
1988
)
Campylobacter pylori subsp. mustelae subsp. nov. isolated from the gastric mucosa of ferrets (Mustela putorius furo), and an emended description of Campylobacter pylori
Int. J. Syst. Bacteriol.
 ,
38
,
367
370
.
13
Fox
J.G.
Taylor
N.S.
Thompson
N.
Borodin
A.
Berg
D.
Shames
B.
(
1993
)
Limited DNA sequence diversity of Helicobacter mustelae, a gastric pathogen of ferrets
Acta Gastro-Enterol. Belgia
 ,
56
,
103
(Suppl.).
14
Goodwin
C.S.
Armstrong
J.A.
Chivers
T.
Peters
M.
Collins
M.D.
Sly
L.
McConnell
W.
Harper
W.E.S.
(
1989
)
Transfer of Campylobacter pylori to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., respectively
Int. J. Syst. Bacteriol.
 ,
39
,
397
405
.
15
Goodwin
C.S.
Worsley
B.W.
(
1993
)
The Helicobacter genus: The history of H. pylori and taxonomy of current species
In
Helicobacter pylori Biology and Clinical Practice
 
Goodwin
C.S.
Worsley
B.W.
, Eds) pp
1
13
CRC Press
,
Boca Raton, FL
.
16
Langenberg
W.
Rauws
E.A.J.
Widjojokusumo
A.
Tytgat
G.N.J.
Zenan
H.C.
(
1986
)
Identification of Campylobacter pyloridis by restriction endonuclease analysis
J. Clin. Microbiol.
 ,
24
,
414
417
.
17
Majewski
S.I.H.
Goodwin
C.S.
(
1988
)
Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation
J. Infect. Dis.
 ,
157
,
465
471
.
18
Nomura
A.
Stemmermann
G.N.
Chyou
P.-H.
Kato
I.
Perrez-Perez
G.I.
Blaser
M.J.
(
1991
)
Helicobacter pylori infection and gastric carcinoma among Japanese Americans in Hawaii
N. Engl. J. Med.
 ,
325
,
1132
1136
.
19
Owen
R.J.
Bickley
J.
Moreno
M.
Costas
M.
Morgan
D.R.
(
1991
)
Biotype and molecular profiles of cytotoxin-producing strains of Helicobacter pylori from antral gastric mucosa
FEMS Microbiol. Lett.
 ,
79
,
199
204
.
20
Parsonnet
J.
Friedman
G.D.
Vandersteen
D.P.
Chang
Y.
Vogelman
J.H.
Orentreich
N.
Silbey
R.K.
(
1991
)
Helicobacter pylori infection and the risk of gastric carcinoma
N. Engl. J. Med.
 ,
325
,
1127
1131
.
21
Sambrook
J.
Fritsch
E.F.
Maniatis
T.
(
1989
)
2nd edn.
, In
Molecular Cloning: A Laboratory Manual
 
Cold Spring Harbor Laboratory
,
Cold Spring Harbor, NY
.
22
Taylor
D.E.
Eaton
M.
Chang
N.
Salama
S.M.
(
1992
)
Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level
J. Bacteriol.
 ,
174
,
6800
6806
.