Abstract

Using pulsed-field gel electrophoresis (PFGE), nine profile types were identified in XbaI digests of a panel of strains of Salmonella enteritidis phage type 4 (PT 4) isolated in England and Wales between 1967 and 1992. When applied to 39 isolates from humans, chickens, poultry products and feed, strains belonging to pulsed-field profile (PFP) 1 predominated. DNA-DNA hybridization studies demonstrated that a band of approximately 57 kb was associated with the presence of the 38 MDa S. enteritidis‘serovar-specific’ plasmid. It is concluded that PFGE provides a method for discriminating strains of S. enteritidis PT 4 suitable for epidemiological investigations.

References

1
Anonymous
(
1994
)
PHLS-SVS Update on Salmonella infection
 
Edition 18
Public Health Laboratory Service and the State Veterinary Service
.
2
Stanley
J.
Jones
C.S.
Threlfall
E.J.
(
1992
)
Evolutionary lines among Salmonella enteritidis phage types are identified by insertion sequence IS200 distribution
FEMS Microbiol. Lett.
 ,
82
,
83
90
.
3
Stanley
J.
Goldsworthy
M.
Threlfall
E.J.
(
1992
)
Molecular phylogenetic typing of pandemic isolates of Salmonella enteritidis
FEMS Microbiol. Lett.
 ,
90
,
153
160
.
4
Threlfall
E.J.
Hampton
M.D.
Chart
H.
Rowe
B.
(
1994
)
Use of plasmid profile typing for surveillance of Salmonella enteritidis phage type 4 from humans, poultry and eggs
Epidemiol. Infect.
 ,
112
,
25
31
.
5
Yan
W.
Chang
W.
Taylor
D.E.
(
1991
)
Pulsed field gel electrophoresis of Campylobacter jejuni and Campylobacter coli genomic DNA and its epidemiological application
J. Infect. Dis.
 ,
163
,
1068
1072
.
6
Schoonmaker
D.
Heimberberger
T.
Birkhead
G.
(
1992
)
Comparison of ribotyping and restriction enzyme analysis using pulsed field gel electrophoresis for distinguishing Legionella pneumophilia isolates obtained during a nosocomial outbreak
J. Clin. Microbiol.
 ,
30
,
1491
1498
.
7
Howard
P.K.
Harsono
K.D.
Luchansky
J.B.
(
1992
)
Differentiation of Listeria monocytogenes, Listeria innocua, Listeria ivanovii and Listeria seeligeri by pulsed field gel electrophoresis
Appl. Environ. Microbiol.
 ,
58
,
709
712
.
8
Liu
S.
Hessel
A.
Sanderson
K.E.
(
1993
)
The XbaI-BlnI-CeuI genomic cleavage map of Salmonella typhimurium LT2 determined by double digestion, end-labelling, and pulsed field gel electrophoresis
J. Bacteriol.
 ,
175
,
4104
4120
.
9
Böhn
H.
Karch
H.
(
1992
)
DNA fingerprinting of Escherichia coli 0157: H7 strains by pulsed field gel electrophoresis
J. Clin. Microbiol.
 ,
30
,
2169
2172
.
10
Ward
L.R.
de Sa
J.D.H.
Rowe
B.
(
1987
)
A phage typing scheme for Salmonella enteritidis
Epidemiol. Infect.
 ,
99
,
291
304
.
11
Chart
H.
Threlfall
E.J.
Rowe
B.
(
1989
)
Virulence of Salmonella enteritidis phage type 4 is related to the possession of a 38 MDa plasmid
FEMS Microbiol. Lett.
 ,
58
,
299
304
.
12
Hinton
M.
Threlfall
E.J.
Rowe
B.
(
1990
)
The invasiveness of different strains of Salmonella enteritidis phage type 4 for young chickens
FEMS Microbiol. Lett.
 ,
70
,
193
196
.
13
Kado
C.I.
Liu
S.T.
(
1981
)
Procedure for the detection of large and small plasmids
J. Bacteriol.
 ,
145
,
1365
1373
.
14
Southern
E.
(
1975
)
Detection of specific sequences among DNA fragments separated by agarose gel electrophoresis
J. Mol. Biol.
 ,
98
,
503
517
.
15
Hinton
M.
Threlfall
E.J.
Rowe
B.
(
1990
)
The invasive potential of Salmonella enteritidis phage types for young chickens
Lett. Appl. Microbiol.
 ,
10
,
237
239
.
16
Maslow
J.N.
Slutsky
A.M.
Arbeit
R.D.
(
1993
)
Application of pulsed field gel electrophoresis to molecular epidemiology
In
Diagnostic Molecular Microbiology. Principles and Applications
 
Persing
D.H.
,
Smith
T.F.
,
Tenover
F.C.
White
T.J.
, Eds)
ASM Publications
,
USA
.