Abstract

A new obligately anaerobic, extremely thermophilic, cellulolytic bacterium is described. The strain designated Tp8T 6331 is differentiated from thermophilic cellulolytic clostridia on the basis of physiological characteristics and phylogenetic position within the Bacillus/Clostridium subphylum of the Gram-positive bacteria. Strain Tp8T 6331 is assigned to a new genus Caldicellulosiruptor, as Caldicellulosiruptor saccharolyticus gen., nov., sp. nov.

References

1
Sissons
C.H.
Sharrock
K.R.
Daniel
R.M.
Morgan
H.W.
(
1987
)
Isolation of cellulolytic anaerobic extreme thermophiles from New Zealand thermal sites
Appl. Environ. Microbiol.
 ,
53
,
832
838
.
2
Taya
M.
Hinoki
H.
Yagi
T.
Kobayashi
T.
(
1988
)
Isolation and characterization of an extremely thermophilic, cellulolytic, anaerobic bacterium
Appl. Microbiol. Bioctech.
 ,
29
,
474
479
.
3
Jones
B.E.
Lindsay
J.M.
(
1989
) In
Proceedings: Academy Workshop on Adaptation of Microorganisms to Extreme Environments
 
An extreme thermophilic, anaerobic eubacterium which hydrolyses cellulose
 , Haren, Netherlands, June 57 1989.
4
Hudson
J.A.
Morgan
H.W.
Daniel
R.M.
(
1990
)
A survey of cellulolytic anaerobic thermophiles from hot springs
Syst. Appl. Microbiol.
 ,
13
,
72
76
.
5
Svetlichny
V.A.
Svetlichnya
T.P.
Chernykh
N.A.
Zavarzin
G.A.
(
1990
)
Anaerocellum thermophilum gen., nov., sp. nov., an extreme thermophilic cellulolytic eubacterium isolated from hot springs in the Valley of Geysers
Mikrobiologiya
 ,
59
,
871
879
.
6
Ng
T.K.
Weimer
P.J.
Zeikus
J.G.
(
1977
)
Cellulolytic and physiological properties of Clostridium thermocellum
Arch. Microbiol.
 ,
114
,
1
7
.
7
Madden
R.H.
(
1983
)
Isolation and characterization of Clostridium stercorarium sp. nov., a cellulolytic thermophile
Int. J. Syst. Bacteriol.
 ,
33
,
837
840
.
8
LeRuyet
P.
Dubourguier
H.C.
Albagnac
G.
Prensier
G.
(
1985
)
Characterization of Clostridium thermolacticum sp. nov. a hydrolytic, thermophilic anaerobe producing high amounts of lactate
Syst. Appl. Microbiol.
 ,
6
,
196
202
.
9
Sukhumavasi
J.
Kunio
O.
Shoichi
S.
Kazue
U.
(
1988
)
Clostridium josui sp. nov., a cellulolytic moderate thermophilic species from thai compost
Int. J. Syst. Bacteriol.
 ,
38
,
179
182
.
10
Jin
F.
Yamasato
K.
Toda
K.
(
1988
)
Clostridium thermocopriae sp. nov., a cellulolytic thermophile from animal feces, compost, soil and a hot spring in Japan
Int. J. Syst. Bacteriol.
 ,
38
,
277
281
.
11
Reynolds
P.H.S.
Sissons
C.H.
Daniel
R.M.
Morgan
H.W.
(
1986
)
Comparison of Cellulolytic activities in Clostridium thermocellum and three thermophilic, cellulolytic anaerobes
Appl. Environ. Microbiol.
 ,
51
,
12
17
.
12
Donnison
A.M.
Brockelsby
C.M.
Morgan
H.W.
Daniel
R.M.
(
1989
)
The degradation of lignocellulosics by extremely thermophilic microorganisms
Biotechnology and Bioengineering
 ,
33
,
1495
1499
.
13
Donnison
A.M.
Brockelsby
C.M.
Morgan
H.W.
(
1986
) In
XIV International Congress of Microbiology
 
Abstract P.14–15 (page 203). Microbe 86
 , Manchester, England
2nd edn.
.
14
Luthi
E.
Bhana Jasmat
N.
Grayling
R.A.
Love
D.R.
Bergquist
P.L.
(
1991
)
Cloning, sequence analysis, and expression in Escherichia coli of a gene coding for a β-mannanase from the extremely thermophilic bacterium ‘Caldocellum saccharolyticum’
Appl. Environ. Microbiol.
 ,
57
,
694
700
.
15
Luthi
E.
Bhana Jasmat
N.
Bergquist
P.L.
(
1990
)
Xylanase from the extremely thermophilic bacterium “Caldocellum saccharolyticum’: overexpression of the gene in Escherichia coli and characterization of the gene product
Appl. Environ. Microbiol.
 ,
56
,
2677
2683
.
16
Rainey
F.A.
Ward
N.L.
Morgan
H.W.
Toalster
R.
Stackebrandt
E.
(
1993
)
Phylogenetic analysis of anaerobic, thermophilic bacteria: aid for their reclassification
J. Bacteriol.
 ,
175
,
4772
4779
.
17
Rainey
F.A.
Janssen
P.H.
Morgan
H.W.
Stackebrandt
E.
(
1994
)
A biphasic approach to the determination of the phenotypic and genotypic diversity of some anaerobic, cellulolytic, thermophilic, rod-shaped bacteria
Ant. van Leeuwenhoek J. Microbiol.
 ,
64
,
341
355
.