Abstract

Muropeptide composition of peptidoglycan from the Gram-negative bacteria Aeromonas sp., Acinetobacter acetoaceticus, Agrobacterium tumefaciens, Enterobacter cloacae, Proteus morganii, Pseudomonas aeruginosa, Pseudomonas putida, Vibrio parahaemolyticus Yersinia enterocolitica and Escherichia coli, was analyzed by HPLC In all instances peptidoglycan was built up from the same subunits. A wide disparity in the relative abundance of muropeptides and all structural parameters was observed. The contribution of LD-A2pm-A2pm cross-linked muropeptides was extremely variable; from 1 to 45% of cross-linked muropeptides. Muropeptides with the dipeptides Lys-Lys or Arg-Lys, indicative of murein-bound (lipo)proteins, were detected in all instances although abundance was very variable.

References

[1]
Schleifer
K.H.
Kandier
O.
(
1972
)
Peptidoglycan tyeps of bacterial cell walls their taxonomic implications
Bacteriol. Rev.
 ,
36
,
407
477
.
[2]
Clarke
A.J.
(
1993
)
Extent of peptidoglycan O acetylation in the tribe Proteeae
J. Bacteriol.
 ,
175
,
4550
4553
.
[3]
Braun
V.
Wu
H.C.
(
1994
)
Lipoproteins, structure, function, biosynthesis and model for protein export
In
Bacterial cell wall
 
Ghuysen
J.-M.
Hakenbeck
R.
, Eds) pp
319
341
Elsevier
,
Amsterdam.
[4]
Mizushima
S.
(
1985
) In
Molecular cytology of Escherichia coli
 
Structure, assembly, and biogenesis of the outer membrane
and
Nanninga
N.
, Ed) pp
39
75
Academic Press
,
London.
[5]
Glauner
B.
(
1988
)
Separation and quantification of muropeptides by high-performance liquid chromatography
Anal. Biochem.
 ,
172
,
451
464
.
[6]
Glauner
B.
Höltje
J.-V.
Schwarz
U.
(
1988
)
The composition of the murein of Escherichia coli
J. Biol. Chem.
 ,
263
,
10088
10095
.
[7]
Pisabarro
A.G.
de Pedro
M.A.
Vźquez
D.
(
1985
)
Structural modification in the peptidoglycan of Escherichia coli associated with changes in the state of growth of the culture
J. Bacteriol.
 ,
161
,
238
242
.
[8]
García-Bustos
J.
Tomasz
A.
(
1987
)
Structure of the peptide network of pneumococcal peptidoglycan
J. Biol. Chem.
 ,
262
,
15400
15405
.
[9]
Caparrós
M.
Pisabarro
A.G.
de Pedro
M.A.
(
1992
)
Effect of D-amino acids on structure and synthesis of peptidoglycan in Escherichia coli
J. Bacteriol.
 ,
174
,
5549
5559
.
[10]
Pittenauer
E.
Schmid
E.R.
Allmaier
G.
Pfanzagl
B.
Löffelhardt
W.
Quintela
J.C.
de Pedro
M.A.
Stanek
W.
(
1993
)
Structural characterization of the cyanellar peptidoglycan of Cyanophora paradoxa by 252Cf plasma desorption mass spectrometry and fast atom bombardment / tandem mass-spectrometry
Biol. Mass Spectrom.
 ,
22
,
524
536
.
[11]
Ottolenghi
A.
Caparrós
M.
de Pedro
M.A.
(
1993
)
Peptidoglycan tripeptide content and cross-linking are altered in Enterobacter cloacae induced to produce AmpC β-lactamase by glycine and D-amino acids
J. Bacteriol.
 ,
175
,
1537
1542
.
[12]
Prats
R.
de Pedro
M.A.
(
1989
)
Normal growth and division of Escherichia coli with a reduced amount of murein
J. Bacteriol.
 ,
171
,
3740
3745
.
[13]
Lennox
E.S.
(
1955
)
Transduction of linked genetic characters of the host by bacteriophage P1
Virology
 ,
1
,
190
206
.
[14]
Lindroth
P.
Mopper
K.
(
1979
)
High performance liquid Chromatographic determination of subpicomole amounts of amino acids by precolumn fluorescence derivatization with o-phthaldialdehyde
Anal. Chem.
 ,
51
,
1667
1674
.
[15]
Glauner
B.
Höltje
J.-V.
(
1990
)
Growth pattern of the murein sacculus of Escherichia coli
J. Biol. Chem.
 ,
263
,
10088
10095
.
[16]
Höltje
J.-V.
Tuomanen
E.
(
1991
)
The murein hydrolases of Escherichia coli properties, functions and impact on the causes of infections in vivo
J. Gen. Microbiol.
 ,
137
,
441
454
.
[17]
Glauner
B.
(
1986
)
Das murein von Escherichia coli
. In
Doctoral thesis
 
University of Tübingen
,
Germany.
[18]
Labischinski
H.
Maidhof
H.
(
1994
)
Bacterial peptidoglycan: overview and evolving concepts. Lipoproteins, structure, function, biosynthesis and model for protein export
In
Bacterial cell wall
 
Ghuysen
J.-M.
Hakenbeck
R.
, Eds) pp
23
38
Elsevier
,
Amsterdam.
[19]
Ursinus
A.
Steinhaus
H.
Höltje
J.-V.
(
1992
)
Purification of a nocardicin-A sensitive LD-carboxypeptidase from Escherichia coli by affinity chromatography
J. Bacteriol.
 ,
174
,
441
446
.