Abstract

Three unlinked genes, TDH1, TDH2 and TDH3, encode the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (triose-phosphate dehydrogenase; TDK) in the yeast Saccharomyces cerevisiae. We demonstrate that the synthesis of the three encoded TDK polypeptides (TDHa, TDHb and TDHc, respectively) is not co-ordinately regulated and that TDHa is only synthesised as cells enter stationary phase, due to glucose starvation, or in heat-shocked cells. Furthermore, the synthesis of TDHb, but not TDHc, is strongly repressed by a heat shock. Hence, the TDHa enzyme may play a cellular role, distinct from glycolysis, that is required by stressed cells.

References

[1]
Holland
J.P.
Holland
M.J.
(
1979
)
The primary structure of a glyceraldehyde-3-phosphate dehydrogenase gene from Saccharomyces cerevisiae
J. Biol. Chem.
 ,
254
,
9839
9845
.
[2]
Holland
J.P.
Holland
M.J.
(
1980
)
Structural comparison of two non-tandemly repeated glyceraldehyde-3-phosphate dehydrogenase genes
J. Biol. Chem.
 ,
255
,
2596
2605
.
[3]
McAlister
L.
Holland
M.J.
(
1985
)
Isolation and characterization of yeast strains carrying mutations in the glycer-aldehyde-3-phosphate dehydrogenase genes
J. Biol. Chem.
 ,
260
,
15013
15018
.
[4]
Holland
J.P.
Labieniec
L.
Swimmer
C.
Holland
M.J.
(
1983
)
Homologous nucleotide sequences at the 5′ termini of mRNAs synthesized from the yeast enolase and glyceralde-hyde-3-phosphate dehydrogenase gene families: the primary structure of a third glyceraldehyde-3-phosphate dehydrogenase gene
J. Biol. Chem.
 ,
258
,
5291
5299
.
[5]
Mountain
H.A.
Korch
C.
(
1991
)
TDH2 is linked to MET3 on chromosome X of Saccharomyces cerevisiae
Yeast
 ,
7
,
873
880
.
[6]
Holland
M.J.
Holland
J.P.
Thill
G.P.
Jackson
K.A.
(
1981
)
The primary structure of two yeast enolase genes: homology between the 5′ non-coding flanking regions of yeast enolase and glyceraldehyde-3-phosphate dehydrogenase genes
J. Biol. Chem.
 ,
256
,
1385
1389
.
[7]
McAlister
L.
Holland
M.J.
(
1985
)
Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes
J. Biol. Chem.
 ,
260
,
15019
15027
.
[8]
Bataillé
N.
Regnacq
M.
Boucherie
H.
(
1991
)
Induction of a heat shock-type response in Saccharomyces cerevisiae following glucose limitation
Yeast
 ,
7
,
367
378
.
[9]
Bataillé
N.
Peypouquet
M.F.
Boucherie
H.
(
1987
)
Identification of polypeptides of the carbon metabolism machinery on the two-dimensional map of Saccharomyces cerevisiae
Yeast
 ,
3
,
11
21
.
[10]
Cleveland
D.W.
Fischer
S.G.
Kirschner
M.W.
Laemmli
U.K.
(
1977
)
Peptide mapping by limited proteolysis in sodium dodecyl sulphate and analysis by electrophoresis
J. Biol. Chem.
 ,
252
,
1102
1106
.
[11]
Boucherie
H.
(
1985
)
Protein synthesis during transition and stationary phases under glucose limitation in Saccharomyces cerevisiae
J. Bacteriol.
 ,
161
,
386
392
.
[12]
Mager
W.H.
Moradas-Ferreria
P.
(
1992
)
Stress response in yeast
Biochem. J.
 ,
290
,
1
13
.
[13]
Fernandes
P.A.
Keen
J.N.
Findlay
J.B.C.
Moradas-Ferreira
P.
(
1992
)
A protein homologous to glyceraldehyde-3-phosphate dehydrogenase is induced in the cell wall of a flocculent Kluyveromyces marxianus
Biochim. Biophys. Acta
 ,
1159
,
67
73
.
[14]
Nickells
R.W.
Browder
L.W.
(
1988
)
A role for glyceraldehyde-3-phosphate dehydrogenase in the development of thermotolerance in Xenopus laevis embryos
J. Cell Biol.
 ,
107
,
1901
1909
.
[15]
Nakai
A.
Satoh
M.
Hirayoshi
K.
Nagata
K.
(
1991
)
Identification of the ATP-binding, heat-inducible protein of Mr= 37,000 as glyceraldehyde-3-phosphate dehydrogenase
Biochem. Biophys. Res. Commun.
 ,
176
,
59
64
.
[16]
Fraenkel
D.G.
(
1992
)
Genetics and intermediary metabolism
Annu. Rev. Genet.
 ,
26
,
159
177
.

Author notes

Lung Biology Center, Box 0854,1001 Potrero Avenue, University of California at San Francisco, San Francisco, CA 94143-0854, USA.