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Summary

The effects of growth media on superoxide dismutase (SOD), catalase and peroxidase were compared in cell-free extracts from four strains of Neisseria meningitidis. The highly virulent strains, DRES-14 and M-1011, were consistently higher in SOD and catalase than the less virulent strains, DRES-03 and DRES-04. Cells grown in brain-heart infusion medium contained the highest levels of catalase. N. meningitidis contained one electrophoretic SOD band which was identified as iron-containing SOD (FeSOD). It was also shown that the presence of catalase could yield an artifactual SOD-band when the SOD activity stain is performed in the presence of 10 mM H2O2, normally required to inactivate and identify FeSODs.

SOD, FeSOD, H2O2-dependent achromatic zone, effect of growth media

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© 1984 Federation of European Microbiological Societies
Issue Section:
Research letter
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