Production of 5α- and 5β-dihydrotestosterone by isolated human axillary bacteria

Summary

The metabolism of testosterone by coryneform bacteria in vitro has been studied. Metabolites identified after derivatization by capillary gas chromatography and further by combined gas chromatography-mass spectrometry were 17β-hydroxy-5α-androstan-3-one and 17β-hydroxy-5β-androstan-3-one. The mass spectral characteristics of the methyl oxime trimethylsilyl ethers of all the 17-hydroxy-androstan-3-one and 3-hydroxy-androstan-17-one isomers are recorded.

Human, axilla, coryneforms, testosterone, 5α(β)-hydrotesterone, gas chromatography-mass spectrometry

 
• aetiocholanolone

  3α-hydroxy-5β-androstan-17-one

 
• androsterone

  3α-hydroxy-5α-androstan-17-one

 
• 5α,β-DHT

  5α(β)-dihydrotestosterone

 
• epiaetiocholanolone

  3β-hydroxy-5β-androstan-17-one

 
• epiandrosterone

  3β-hydroxy-5α-androstan-17-one

 
• GC

  capillary gas chromatography

 
• 17-hydroxypregnenolone

  3β,17α-dihydroxy-5-pregnen-20-one

 
• MO

  methyl oxime

 
• MS

  mass spectrometry

 
• MU

  methylene units

 
• pregnenolone

  3β-hydroxy-5-pregnen-20-one

 
• testosterone

  17β-hydroxy-4-androsten-3-one

 
• TLC

  thin-layer chromatography

 
• TMS

  trimethylsilyl