Reconstitution by a butyrolactone autoregulator of the parental protein pattern in an asporogenous mutant of Streptomyces griseus

Summary

The asporogenous anthracycline-negative mutant 86 (Amy⁻Ant⁻) of Streptomyces griseus was distinguishable from the parental strain JA5142 by an altered composition of the mycelial proteins. Addition of trans-2-(6′-methylheptanol-1′-yl)-3-hydroxymethyl-4-butanolide (factor I) to submerged cultures caused the adaptation of its protein pattern to that of the parental strain, concomitant with the restoration of anthracycline biosynthesis and morphological changes. In addition to the other effects of factor I on metabolic activity, the observed changes could be accounted for, at least partly, by alterations in intramycelial proteolysis. On the other hand, in the presence of factor I, some protein concentrations remained unaffected, supporting the view that a pleiotropic gene is involved in the synthesis of the autoregulatory effector by the parental strain S. griseus JA5142.

Asporogenous mutant of S. griseus, protein pattern, effect of butyrolactone autoregulators