Expression of Escherichia coli PhoE protein in avirulent Salmonella typhimurium aroA and galE strains

Summary

Escherichia coli K-12 PhoE protein is found to be normally expressed and incorporated into the outer membrane of two avirulent Salmonella typhimurium strains, G30 and SHaroA. A hybrid protein which contains an insertion of an antigenic epitope of VP1 protein of foot-and-mouth disease virus into the PhoE protein, was also normally assembled into the Salmonella outer membranes. In the case of the G30 stain, which carries a galE mutation, the inserted epitope is accessible to antibodies in intact cells. In contrast, the epitope is less accessible in the case of the SHaroA strain, probably due to the shielding effect of the O-antigen in this strain.

Outer membrane protein, Salmonella typhimurium, PhoE protein, Genetic vector, Antigen exposition