Structural organization of the membrane-bound hydrogenase isolated from Alcaligenes eutrophus as revealed by electron microscopy

Summary

Electron microscopy of negatively stained samples of the membrane-bound hydrogenase isolated from Alcaligenes eutrophus was used to obtain enzyme images with an estimated resolution of 2.5 nm. The two subunits with shapes similar to the letter ‘U’ making up the enzyme could be seen to be joined in two planes orthogonal to each other, making contact with their concave sides. In face-on view, the particle exhibited bilateral symmetry.

Membrane-bound hydrogenase, Alcaligenes eutrophus, Electron microscopy