Summary

Constitutive expression of the S. typhimurium histidine operon causes multiple phenotypic changes including strong flamentation. However, the SOS regulatory network is not involved in the inhibition of cell division. The possibility of SOS- independent activation of sulA gene transcription has been ruled out using sulA-lacZ fusions. These results suggest that existence of a pathway of division inhibition unrelated to sulA and not regulated by the SOS system.

References

[1]
Ely
B.
(
1984
)
Genetics
 ,
78
,
593
606
.
[2]
Johnston
H.M.
Barnes
W.
Chumley
F.
Bossi
L.
Roth
J.R.
(
1980
),
77
, In
Proc. Natl. Acad. Sci. U.S.A.
 , pp
508
512
.
[3]
Johnston
H.M.
Roth
J.R.
(
1981
)
J. Mol. Biol.
 ,
145
,
735
756
.
[4]
Roth
J.R.
Antón
D.N.
Hartman
P.E.
(
1966
)
J. Mol. Biol.
 ,
22
,
305
323
.
[5]
Antón
D.
(
1968
)
J. Mol. Biol.
 ,
33
,
533
546
.
[6]
Brenner
M.
Ames
B.N.
(
1972
)
J. Biol. Chem.
 ,
247
,
1080
1088
.
[7]
Bossi
L.
(
1983
)
Mol. Gen. Genet.
 ,
192
,
163
170
.
[8]
Fink
G.R.
Klopotowski
T.
Ames
B.N.
(
1967
)
J. Mol. Biol.
 ,
30
,
61
95
.
[9]
Murray
L.
Hartman
P.E.
(
1972
)
Can. J. Microbiol.
 ,
18
,
671
681
.
[10]
Casadesús
J.
Roth
J.R.
(
1989
)
Mol. Gen. Genet.
 ,
216
,
210
216
.
[11]
Schmieger
H.
(
1971
)
Mol. Gen. Genet.
 ,
110
,
378
381
.
[12]
Salles
B.
Weiseman
J.M.
Weinstock
G.M.
(
1987
)
J. Bacteriol.
 ,
169
,
5028
5034
.
[13]
Elledge
S.J.
Walker
G.C.
(
1983
)
J. Mol. Biol.
 ,
164
,
175
192
.
[14]
Schneider
K.
Beck
C.F.
(
1987
)
Meth. Enzymol.
 ,
153
,
452
460
.
[15]
Henning
U.
Royer
H.D.
Teather
R.M.
Hindenach
I.
Hollenberg
C.P.
(
1979
),
76
, In
Proc. Natl. Acad. Sci. U.S.A.
 , pp
4360
4364
.
[16]
Vogel
H.J.
Bonner
D.M.
(
1956
)
J. Biol. Chem.
 ,
218
,
97
106
.
[17]
Davis
R.W.
Botstein
D.
Roth
J.R.
(
1980
)
Advanced bacterial genetics
 
Cold Spring Harbor Laboratory
Cold Spring Harbor, New York
.
[18]
Miller
J.H.
(
1972
)
Experiments in bacterial genetics
 
Cold Spring Harbor Laboratory
Cold Spring Harbor, New York
.
[19]
Birnboim
H.C.
Doly
J.
(
1979
)
Nucl. Acids Res.
 ,
7
,
1513
1519
.
[20]
Barbé
J.
(
1988
)
ISI Atlas of Science: Biochemistry
 ,
1
,
175
181
.
[21]
Elespuru
R.K.
(
1987
)
Environm. Molec. Mutagenesis
 ,
10
,
97
116
.
[22]
Lutkenhaus
J.L.
(
1983
)
J. Bacteriol.
 ,
154
,
1339
1346
.
[23]
Burton
P.
Holland
B.
(
1983
)
Mol. Gen. Genet.
 ,
190
,
309
314
.
[24]
Taschner
P.E.M.
Verest
J.G.J.
Woldringh
C.L.
(
1987
)
J. Bacteriol.
 ,
169
,
19
25
.

Author notes

1
Dept. Genética i Microbiologia, Universitat Autónoma de Barcelona, 08193 Bellaterra, Barcelona, Spain.