Summary

The rates of germ tube formation from growing and non-growing yeast cells of Candida albicans were investigated using a protocol for dimorphism regulated by temperature and pH. Stationary-phase cells formed germ tubes less rapidly than yeast cells that were preincubated in fresh growth medium prior to induction of dimorphism by an upshift in temperature or pH. On the basis of experiments using inhibitors of macromolecular biosyntheses it is suggested that the accelerated growth kinetics required de novo RNA and protein biosynthesis, but not DNA synthesis. The results suggest that metabolically active yeast cells are better able to undergo dimorphism than non-growing cells.

REFERENCES

[1]
Odds
F.C.
(
1988
)
Candida and Candidosis
 
Bailliere Tindall
London
.
[2]
Gow
N.A.R.
Gooday
G.W.
(
1987
)
Crit. Rev. Microbiol.
 ,
15
,
73
78
.
[3]
Soll
D.R.
Herman
M.A.
(
1983
)
J. Gen. Microbiol.
 ,
129
,
2809
2824
.
[4]
Soll
D.R.
Bedell
G.W.
(
1978
)
J. Gen. Microbiol.
 ,
108
,
173
180
.
[5]
Mitchell
L.H.
Soll
D.R.
(
1979
)
Exper. Cell Res.
 ,
120
,
167
179
.
[6]
Bell
W.M.
Chaffin
W.L.
(
1980
)
Can. J. Microbiol.
 ,
26
,
102
105
.
[7]
Ahrens
I.C.
Price
M.R.
Daneo-Moore
L.
Buckley
H.R.
(
1983
)
J. Gen. Microbiol.
 ,
129
,
3001
3006
.
[8]
Mattia
E.
Cassone
A.
(
1979
)
J. Gen. Microbiol.
 ,
113
,
439
442
.
[9]
Bell
W.M.
Chaffin
W.L.
(
1983
)
Mycopathologia
 ,
84
,
41
44
.
[10]
Buffo
J.
Herman
M.A.
Soll
D.R.
(
1984
)
Mycopathologia
 ,
85
,
21
30
.
[11]
Lee
K.L.
Buckley
H.R.
Campbell
H.R.
(
1975
)
Sabouraudia
 ,
13
,
148
153
.
[12]
Santiago
T.C.
Purvis
I.J.
Bettany
A.J.E.
Brown
A.J.P.
(
1986
)
Nucleic Acids Res.
 ,
14
,
8347
8360
.
[13]
Chaffin
W.L.
Wheeler
D.E.
(
1981
)
Can. J. Microbiol.
 ,
27
,
131
137
.
[14]
Soll
D.R.
Stasi
M.
Bedell
G.
(
1978
)
Exp. Cell Res.
 ,
116
,
207
215
.
[15]
Lingappa
B.T.
Prasad
M.
Lingappa
Y.
(
1967
)
Science
 ,
163
,
192
194
.
[16]
Hazen
K.C.
Cutler
J.E.
(
1983
)
Exp. Mycol.
 ,
7
,
182
187
.
[17]
Soll
D.R.
Bedell
G.W.
Brummel
M.
(
1981
)
Infect. Immun.
 ,
32
,
1139
1147
.
[18]
Bedell
G.W.
Anderson
R.V.
(
1985
)
Mycopathologia
 ,
92
,
161
167
.
[19]
Yamaguchi
H.
(
1975
)
J. Gen. Microbiol.
 ,
86
,
370
372
.
[20]
Soll
D.R.
(
1985
)
Curr. Top. Med. Mycol.
 ,
1
,
258
285
.
[21]
Anderson
J.M.
Soll
D.R.
(
1984
)
Infect. Immun.
 ,
46
,
13
21
.