Summary

Allelic variations in the chromosomal genome of 120 isolates of motile Aeromonas (A. hydrophila, A. sobria and A. caviae) and eight reference strains with one Plesiomonas shigelloides were assessed by analysis of electrophoretically demonstrable polymorphism in 16 genes encoding metabolic enzymes. The strains were collected from humans (n= 59) and from the aquatic environment (n= 61) in canton Tessin, Switzerland. Clustering of the electrophoretic types (ET) from a matrix of pairwise genetic distances, based on the 16 enzyme loci, confirmed the genetic distinctness of the three species. Furthermore, A. hydrophila and A. sobria were divided in three and two groups respectively. For each species clinical strains were well differentiated from those collected in the environment.

REFERENCES

[1]
Nishikawa
Y.
Kishi
T.
(
1988
)
Epidemiol. Infect.
 ,
101
,
213
223
.
[2]
Knochel
S.
Jeppesen
C.
(
1990
)
Int. J. Food Microbiol.
 ,
10
,
317
322
.
[3]
Altwegg
M.
Geiss
H.K.
(
1989
)
CRC Crit. Rev. Microbiol.
 ,
16
,
253
286
.
[4]
Ljungh
Å.
Wadstrøm
T.
(
1982
)
Pharmacol. Ther.
 ,
15
,
339
354
.
[5]
Cahill
M.M.
(
1990
)
J. Appl. Bacteriol.
 ,
69
,
1
16
.
[6]
Popoff
M.
(
1984
) In
Bergey's Manual of Systematic Bacteriology
 
Krieg
N.R.
Holt
J.G.
, Eds) Vol.
1
, pp
545
548
Williams and Wilkins
,
Baltimore, MD
.
[7]
Popoff
M.
Véron
M.
(
1976
)
J. Gen. Microbiol.
 ,
94
,
11
22
.
[8]
Shaw
D.H.
Hodder
H.J.
(
1978
)
Can. J. Microbiol.
 ,
24
,
864
868
.
[9]
Popoff
M.Y.
Coynault
C.
Kiredjian
M.
Lemelin
M.
(
1981
)
Curr. Microbiol.
 ,
5
,
109
114
.
[10]
Allen
D.A.
Austin
B.
Colwell
R.R.
(
1983
)
Int. J. Syst. Bacteriol.
 ,
33
,
599
604
.
[11]
Hickman Brenner
F.W.
MacDonald
K.L.
Steigerwalt
A.G.
Fanning
G.R.
Brenner
D.J.
Farmer
J.J.
(
1987
)
J. Clin. Microbiol.
 ,
25
,
900
906
.
[12]
Schubert
R.H.
Hegazi
M.
(
1988
)
Zentralbl. Bakteriol. Mikrobiol. Hyg. (A)
 ,
268
,
34
39
.
[13]
Hickman Brenner
F.W.
Fanning
G.R.
Arduino
M.J.
Brenner
D.J.
(
1988
)
J. Clin. Microbiol.
 ,
26
,
1561
1564
.
[14]
De Meuron
P.-A.
Peduzzi
R.
(
1979
)
R. Zbl. Vet. Med. B
 ,
26
,
153
167
.
[15]
Peduzzi
R.
Demarta
A.
(
1986
)
Schweiz. Z. Hydrol.
 ,
48
,
161
170
.
[16]
Selander
R.K.
Caugant
D.A.
Ochman
H.
Musser
J.M.
Gilmour
M.N.
Whittam
T.S.
(
1986
)
Appl. Environ. Microbiol.
 ,
51
,
872
884
.
[17]
Altwegg
M.
Altwegg-Bissig
R.
Demarta
A.
Peduzzi
R.
Reeves
M.W.
Swaminathan
B.
(
1988
)
J. Diarrhoeal Dis. Res.
 ,
6
,
88
94
.
[18]
Picard-Pasquier
N.
Picard
B.
Heeralal
S.
Krishnamoorthy
R.
Goullet
P.
(
1990
)
J. Gen. Microbiol.
 ,
136
,
1655
1666
.
[19]
Arthur
M.
Arbeit
R.D.
Kim
C.
Beltran
P.
Crowe
H.
Steinbach
S.
Campanelli
C.
Wilson
R.A.
Selander
R.K.
Goldstein
R.
(
1990
)
Infect. Immun.
 ,
58
,
471
479
.
[20]
Sneath
P.H.A.
Sokal
R.R.
(
1973
)
Numerical Taxonomy
 
W.H. Freeman
San Francisco, CA
.