Transformation of a plasmid-free, genital tract isolate of Chlamydia trachomatis with a plasmid vector carrying a deletion in CDS6 revealed that this gene regulates inclusion phenotype

The development of a plasmid-based genetic transformation protocol for Chlamydia trachomatis provides the basis for the detailed investigation of the function of the chlamydial plasmid and its individual genes or coding sequences (CDS). In this study we constructed a plasmid vector with CDS6 deleted (pCDS6KO) from the original Escherichia coli/C. trachomatis shuttle vector pGFP::SW2. pCDS6KO was transformed into a clinical isolate of C. trachomatis from Sweden that is plasmid-free (C. trachomatis SWFP–). Penicillin-resistant transformants expressing the green fluorescent protein were selected. These transformants did not stain with iodine, indicating that this property is regulated by CDS6 or its gene product. In addition, mature inclusions of C. trachomatis SWFP– transformed by pCDS6KO displayed an identical morphological phenotype to the untransformed plasmid-free recipient host. In this phenotype the morphology of inclusions was altered with the chlamydiae lining the periphery of the inclusion leaving a ‘hole’ in the centre. These green fluorescent inclusions appear ‘doughnut-shaped’ with an empty centre when examined under blue light, giving rise to a characteristic ‘black hole’ phenotype. Our study demonstrates the power of the new genetic system for investigating chlamydial gene function using gene deletion technology.

Chlamydia trachomatis is a major human pathogen responsible for a variety of important diseases including trachoma and genital tract infections. It is an obligate intracellular pathogen that replicates within a specialized cytoplasmic structure known as an inclusion and has a unique developmental cycle (Ward, 1983). Cell infection is initiated by the elementary body or EB. EBs are taken up into a specialized vesicle that becomes a mature inclusion within which they differentiate into the larger replicative noninfectious form of the microorganism, the reticulate body or RB. RBs undergo 8-10 divisions within the inclusion and then condense to become EBs (Lambden et al., 2006). RBs regulate the inclusion and control of many cellular factors through secretion of proteins from the inclusion into the cell cytoplasm (Kokes & Valdivia, 2012). Chlamydia trachomatis isolates are divided into at least 15 serovars which are associated with the different disease pathologies. Serovars A, B and C are associated with blinding trachoma and serovars D-K are responsible for nondisseminating sexually transmitted infections. Thus, these serovars (A-K) infect epithelial cells and are collectively known as the 'trachoma' biovar. By contrast, C. trachomatis serovars L1, L2 and L3 cause a highly invasive, rare, sexually transmitted disease known as lymphogranuloma venereum (LGV) and thus belong to the LGV biovar.
LGV isolates are a popular choice for laboratory work as they usually grow faster although they do have subtly different, less compact inclusion morphology compared with trachoma biovar isolates. These biological differences are also supported by phylogenetic and genome differences, reinforcing the clinical observations that LGV and trachoma isolates can confidently be split into discrete disease entities (Harris et al., 2012).
Most isolates of C. trachomatis carry a 7.5-kb plasmid (Thomas et al., 1997). The plasmid encodes eight coding sequences (CDS) which are arranged so that all but one of the genes are transcribed in the same direction. Assignment of biological function to the eight plasmid genes has been difficult because of the absence of a simple means to manipulate the chlamydial genome (Rockey, 2011). Bioinformatic analyses have indicated that CDS1-3 are probably involved in plasmid replication as CDS1 and 2 are organized into an iteron-type structure regulating the plasmid origin of replication and both sequences share some homology, and are also distantly related to other bacterial recombinases/ replication enzymes, indicating that these genes are involved in regulating plasmid replication. CDS3 has homology to the Escherichia coli DnaB gene, which encodes an enzyme involved in unwinding DNA during replication (Hatt et al., 1988). CDS7 and 8 display sequence homology with other plasmid partition proteins and thus are likely to be essential for plasmid maintenance. Comparison of the phylogenies of plasmids and chromosomes suggests that plasmid evolution remains closely linked to its cognate host chromosome and thus that plasmids and chromosomes are coinherited (Seth-Smith et al., 2009).
Recently, we developed a simple transformation protocol based on using the chlamydial plasmid as a vector and calcium chloride (CaCl 2 ) treatment of LGV elementary bodies to render them competent to take up plasmid DNA (Wang et al., 2011). LGV isolates were selected as they grow faster than trachoma biovars and are easier to transform. We initially used an LGV plasmid and a matched LGV host but we also used a mismatched trachoma plasmid (from the Swedish new variant)/LGV recipient host pair in this work to prove transformation across the biovars was possible.
Our longer term aim is to define the minimal sequences necessary for a shuttle vector by selected deletion. The ability to selectively delete regions of the plasmid also affords the possibility of dissecting further the properties and traits associated with them. A key part of our experimental design was to perform these transformations with plasmidfree isolates, thereby removing the possibility of complementation of the deletions by the endogenous plasmid. A second and important part of our experimental design was to accurately ascribe and match phenotype to genetics, so we considered it essential to use the correctly paired plasmid/recipient host combination to ensure optimal expression of the plasmid-related phenotype.
Naturally occurring plasmid-free C. trachomatis are exceedingly rare and only four live isolates have been described (Peterson et al., 1990;Persson et al., 1996;Farencena et al., 1997;Stothard et al., 1998). It is possible to cure Chlamydia of its plasmid by treatment with Novobiocin (O'Connell & Nicks, 2006). These naturally occurring plasmid-free and plasmid-cured Chlamydia all display an unusual inclusion morphology and fail to accumulate glycogen. Studies on natural plasmid-free and plasmid-cured C. trachomatis have indicated that the plasmid is a virulence factor and its absence is associated with reduced disease severity (Carlson et al., 2008;Olivares-Zavaleta et al., 2010;Kari et al., 2011;O'Connell et al., 2011).
Transformation of plasmid-free C. trachomatis L2 with complete wild-type coding sequences (as part of the shuttle vector pGFP::SW2) restored both the ability to accumulate glycogen and wild-type inclusion morphology, proving that these traits are plasmid-encoded (Wang et al., 2011). However, our starting point for the current work was to use a genital tract (trachoma biovar) isolate with a matched genital tract plasmid; in this way we planned to optimize expression of the plasmid-associated phenotypes. For this we used a naturally occurring plasmid-free genital tract isolate from Sweden, C. trachomatis SWFP-, as a recipient host (Persson et al., 1996). We transformed C. trachomatis SWFP-using the CaCl 2 -based transformation protocol developed for C. trachomatis L2 with the following modifications: an increased amount of C. trachomatis SWFP-EB for transformation (plasmid-free strains are sensitive to the CaCl 2 treatment) and centrifugation (754 g for 30 min) to initiate cell infection.
As the replication/maintenance proteins encoded by CDS1-3 and 7 and 8 are unlikely to have dual functions, we considered that chlamydial phenotypes would be determined by the other genes. Initially we attempted to delete CDS4 from the shuttle vector pGFP::SW2. However, it has not proven possible to recover C. trachomatis transformants when this gene was deleted from the shuttle vector. Thus it appears that CDS4 may have a role in an as yet unknown essential plasmid function.
As CDS6 is the smallest plasmid gene, we also devised a simple strategy to make a construct, pCDS6KO, with this CDS deleted in its entirety from the original plasmid vector pGFP::SW2. The deleted region in pGFP::SW2 starts after CDS5 stop codon TAA and ends before CDS6 stop codon TAG (Fig. 1a). pCDS6KO was constructed by inserting the 860-bp PacI-SpeI fragment from CDS5 PCR product into the PacI-SpeI sites of pCDS5&6KO, so that CDS5 returned to its original location. The CDS5 PCR was performed by using primers ORF5_F(PacI) (5′-CAAGAATCTATTAATT AATAGCAAGCTTGAAAC-3′) and ORF5_R(SpeI) (5′-AAAAAAACTAGTTTAAGCGTTTGTTTGAGGTATTACC-3′), and pGFP::SW2 as DNA template. As a result, a 375-bp DNA fragment (including CDS6 and its promoter) was deleted from pGFP::SW2, and replaced with a unique SpeI site. The PCR regions and the ligation sites of both pCDS5&6KO and pCDS6KO were verified by sequence analysis. All genetic manipulations and containment work was approved under the UK Health and Safety Executive Genetically Modified Organisms (contained use) regulations 2000 notification no. GM57,10.1 entitled 'Genetic transformation of Chlamydiae'.
We were able reproducibly to recover transformants of C. trachomatis SWFP-with pGFP::SW2 and the two new vectors carrying the CDS6 deletion, pCDS5&6KO and pCDS6KO. The properties of transformants were analysed by phase contrast microscopy, green fluorescence protein (GFP) imaging and iodine staining (Fig. 1b). All three transformants expressed GFP. However, the distribution of green fluorescence was different. In C. trachomatis SWFP-transformed with pCDS5&6KO or pCDS6KO, green fluorescence was concentrated in the perimeter of the inclusions, leaving a 'black hole' in the centre (Fig. 1b). The phase contrast images were identical to the untransformed plasmid-free parental strain SWFP-. Both of the transformants were iodine stain-negative (Fig. 1B). Transformed C. trachomatis SWFP-with pGFP::SW2 displayed normal inclusion morphology and was iodine stain-positive, indicating the accumulation of glycogen (Fig. 1b).
Inclusions of C. trachomatis SWFP-transformed with CDS6 deletion vectors could not be stained with iodine, indicating that glycogen accumulation was dependent on the presence of CDS6; and the inclusion morphology was identical to the untransformed recipient parental C. trachomatis SWFP-proving that CDS6 was essential for maintaining the normal inclusion morphology. Thus, we have conclusively shown that the C.trachomatis plasmid CDS6 is responsible for the plasmid-associated phenotype of glycogen accumulation/biosynthesis. Transcriptional analyses of the CDS6 region have not indicated the presence of highly abundant noncoding RNAs characteristic of other regions of the plasmid (Ricci et al., 1995;Albrecht et al., 2010). Further molecular/genetic analysis of CDS6 is required to elucidate the molecular mechanism(s) by which it or its encoded polypeptide regulates glycogen biosynthesis and influences inclusion morphology. A simple explanation of the 'black hole' inclusion morphology could be that the growth rate of the CDS6 knockout mutant(s) is reduced at the mid stage of the developmental cycle, generating a distinctive inclusion whose membrane grows normally and would yield doughnut-shaped inclusions with replicating RBs at the periphery. This is consistent with the contact-dependent development model of intracellular development (Wilson et al., 2006(Wilson et al., , 2009Peters et al., 2007). It is possible that the protein encoded by CDS6 is also the factor responsible for conferring the virulence properties associated with the C. trachomatis plasmid. Future work will include assessing the properties of the C. trachomatis transformants described here in animal models of infection.