- Split View
-
Views
-
CiteCitation
Deborah Shaw, Ian R. Poxton, John R.W. Govan; Biological activity of Burkholderia (Pseudomonas) cepacia lipopolysaccharide, FEMS Immunology & Medical Microbiology, Volume 11, Issue 2, 1 April 1995, Pages 99–106, https://doi.org/10.1111/j.1574-695X.1995.tb00095.x
Download citation file:
© 2018 Oxford University Press
Close -
Share
Abstract
Burkholderia cepacia has emerged as an important multiresistant pathogen in cystic fibrosis (CF), associated in 20% of colonised patients with a rapid and fatal decline in lung function. Although knowledge of B. cepacia epidemiology has improved, the mechanisms involved in pathogenesis remain obscure. In this study, B. cepacia lipopolysaccharide (LPS) was assessed for endotoxic potential and the capacity to induce tumour necrosis factor (TNF). LPS preparations from clinical and environmental isolates of B. cepacia and from the closely related species Burkholderia gladioli exhibited a higher endotoxic activity and more pronounced cytokine response in vitro compared to preparations from the major CF pathogen Pseudomonas aeruginosa. This study may help to explain the vicious host immune response observed during pulmonary exacerbations in CF patients colonised by B. cepacia and lead to therapeutic advances in clinical management.
Burkholderia cepacia, Pseudomonas cepacia, Cystic fibrosis, Lipopolysaccharide, Tumour necrosis factor
References
[1]
Govan
J.R.W.
Nelson
J.W.
1992
) Microbiology of lung infection in cystic fibrosis
. Br. Med. Bull.
48
, 912
–930
.[2]
Prince
A.
1986
) Antibiotic resistance in Pseudomonas species
. J. Pediatr.
108
, 830
–834
.[3]
Lewin
C.
Doherty
C.
Govan
J.
1993
) In vitro activities of meropenem, PD 127391 and PD 131628
. Antimicrob. Agents Chemother.
37
, 123
–125
.[4]
Govan
J.R.W.
Brown
P.H.
Maddison
J.
Doherty
C.J.
Nelson
J.W.
Dodd
M.
Greening
A.P.
Webb
A.K.
1993
) Evidence for transmission of Pseudomonas cepacia by social contact in cystic fibrosis
. Lancet
342
, 15
–19
.[5]
Isles
A.
Maclusky
I.
Corey
M.
Gold
R.
Prober
C.
Fleming
P.
Levison
H.
1984
) Pseudomonas cepacia infection in cystic fibrosis: an emerging problem
. J. Pediatr.
104
, 206
–210
.[6]
Rosenstein
B.J.
Hall
D.E.
1980
) Pneumonia and septicemia due to Pseudomonas cepacia in a patient with cystic fibrosis
. John Hop. Med. J.
147
, 188
–189
.[7]
Nelson
J.W.
Butler
S.L.
Krieg
D.
Govan
J.R.W.
1994
) Virulence factors of Burkholderia cepacia
. FEMS Immunol. Med. Microbiol.
8
, 89
–98
.[8]
Rietschel
E.Th.
Kirikae
T.
Schade
F.U.
Ulmer
A.J.
Holst
O.
Brade
H.
Schmidt
G.
Mamat
U.
Grimmecke
H.
Kusumoto
S.
Zahringer
U.
1993
) The chemical structure of bacterial endotoxin in relation to bioactivity
. Immunobiology
187
, 169
–190
.[9]
Rees
R.C.
1991
) Cytokines: their role in regulating immunity and the response to infection
. Rev. Med. Microbiol.
3
, 9
–14
.[10]
Greally
P.
Hussein
M.J.
Cook
A.J.
Sampson
A.P.
Piper
P.J.
Price
J.F.
1993
) Sputum tumour necrosis factor-α and leukotriene concentrations in cystic fibrosis
. Arch. Dis. Child
68
, 389
–392
.[11]
Wilson
C.B.
Franklin
T.
Smith
A.L.
Casey
S.
Kushmerick
P.
Hedges
D.
Williams-Warren
J.
Sherry
B.L.
Ramsey
B.
1993
) Increased TNF-α and IL-1β production in CF patients with clinical deterioration
. 7th North American Cystic Fibrosis Conference, Dallas, Symposium Session Summaries
, Abstract S8.3
, pp. 119
–120
.[12]
Christenson
J.C.
Welch
D.F.
Mukwaya
G.
Muszynski
M.J.
Weaver
R.E.
Brenner
D.J.
1989
) Recovery of Pseudomonas gladioli from respiratory tract specimens of patients with cystic fibrosis
. J. Clin. Microbiol.
27
, 270
–273
.[13]
Simpson
I.N.
Finlay
J.
Winstanley
D.J.
Dewhurst
N.
Nelson
J.W.
Butler
S.L.
Govan
J.R.W.
1994
) Multiresistance isolates possessing characteristics of both Burkholderia (Pseudomonas) cepacia and Burkholderia gladioli from patients with cystic fibrosis
. J. Antimicrob. Chemother
34
, 353
–361
.[14]
Govan
J.R.W.
Harris
G.
1985
) Typing of Pseudomonas cepacia by bacteriocin susceptibility and production
. J. Clin. Microbiol.
22
, 490
–494
.[15]
Grothes
D.
Koopmann
U.
von DerHardt
H.
Tümmler
B.
1988
) Genome fingerprinting of Pseudomonas aeruginosa indicates colonization of cystic fibrosis siblings with closely related strains
. J. Clin. Microbiol.
26
, 1973
–1977
.[16]
Westphal
O.
Luderitz
O.
1954
) Chemische erforschung von lipopolysacchariden gramnegativer bakterien
. Angew. Chem.
66
, 407
–414
.[17]
Laemmli
U.K.
1970
) Cleavage of structural proteins during the assembly of the head protein of bacteriophage T4
. Nature
227
, 680
–685
.[18]
Tsai
C-M.
Frasch
C.E.
1982
) A sensitive silver stain for detecting lipopolysaccharides in polyacrylamide gels
. Anal. Biochem.
119
, 115
–119
.[19]
Hancock
I.C.
Poxton
I.R.
1988
) Bacterial Cell Surface Techniques
. John Wylie and Sons
, Chichester
.[20]
Towbin
H.
Staehelin
T.
Gordon
J.
1979
) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications
. Proc. Natl. Acad. Sci. USA
76
, 4350
–4354
.[21]
Beckmann
I.
van Eijk
H.G.
Meisel-Mikokijczyk
F.
Wallenburg
H.C.
1989
) Detection of 2-keto-3-deoxyoctonate in endotoxins isolated from six references strains of the Bacteroides fragilis group
. Int. J. Biochem.
21
, 661
–666
.[22]
Lowry
O.H.
Rosebrough
N.J.
Farr
A.L.
Randall
R.J.
1951
) Protein measurement with the Folin Phenol reagent
. J. Biol. Chem.
193
, 265
–275
.[23]
Chen
P.S.
Toribara
T.Y.
Warner
H.
1956
) Microdetermination of phosphorus
. Anal. Chem.
28
, 1756
–1761
.[24]
Dubois
M.
Gilles
K.A.
Hamilton
J.K.
Rebers
P.A.
1956
) Colorimetric method for the determination of sugars and related substances
. Anal. Chem.
28
, 350
–356
.[25]
McKevitt
A.I.
Woods
D.E.
1984
) Characterization of Pseudomonas cepacia isolates from patients with cystic fibrosis
. J. Clin. Microbiol.
19
, 291
–293
.[26]
Gressner
A.R.
Mortensen
J.E.
1990
) Pathogenic factors of Pseudomonas cepacia isolates from patients with cystic fibrosis
. J. Med. Microbiol.
33
, 115
–120
.[27]
Cox
A.D.
Wilkinson
S.G.
1989
) Structures of the O-specific polymers from the lipopolysaccharides of the reference strains for Pseudomonas cepacia serogroups 03 and 05
. Carbohydr. Res.
195
, 123
–129
.[28]
Cox
A.D.
Wilkinson
S.G.
1990
) Structure of the putative O antigen containing 2-amino-2-deoxy-l-glucose in the refernce strain for Pseudomonas cepacia serogroup 01
. Carbohydr. Res.
195
, 295
–301
.[29]
Cox
A.D.
Wilkinson
S.G.
1991
) Ionizing groups in lipopolysaccharides of Pseudomonas cepacia in relation to antibiotic resistance
. Mol. Microbiol.
5
, 641
–646
.[30]
Manthey
C.L.
Vogel
S.N.
1992
) The role of cytokines in host responses to endotoxin
. Rev. Med. Microbiol.
3
, 72
–79
.[31]
Fomsgaard
A.
1990
) Antibodies to LPS in diagnosis
. APMIS
vol 98
, (Suppl. 18
), 24
–27
.[32]
Burkhead
K.D.
Schisler
D.A.
Slininger
P.J.
1994
) Pyrrolnitrin production by biological control agent Pseudomonas cepacia B37w in culture and in colonized wounds of potatoes
. Appl. Environ. Microbiol.
60
, 2031
–2039
.[33]
Janisiewicz
W.J.
Roitman
J.
1988
) Biological control of blue and gray mold on apple and pear with Pseudomonas cepacia
. Phytopath.
78
, 1697
–1700
.[34]
Aoki
M.
Uehara
K.
Koseki
K.
Tsuji
K.
Iijima
M.
Ono
K.
Samejima
T.
1991
) An antimicrobial substance produced by Pseudomonas cepacia B5 against the bacterial wilt disease pathogen, Pseudomonas solanacearum
. Agric. Biol. Chem.
55
, 715
–722
.[35]
Folsom
B.R.
Chapman
P.J.
Pritchard
P.H.
1990
) Phenol and trichloroethylene degradation by Pseudomonas cepacia G4: Kinetics and interactions between substrates
. Appl. Environ. Microbiol.
56
, 1279
–1285
.[36]
Bevivino
A.
Tabacchioni
S.
Chiarini
L.
Carusi
M.V.
Del Gallo
M.
Visca
P.
1994
) Phenotypic comparison between rhizosphere and clinical isolates of Burkholderia cepacia
. Microbiology
140
, 1069
–1077
.[37]
List No. 45
. (1993
) Validation of the publication of new names and new combinations previously effectively published outside the IJSB
. Int. J. Syst. Bacteriol.
43
, 398
–399
.[38]
Manniello
J.M.
Heymann
H.
Adair
F.W.
1979
) Isolation of atypical lipopolysaccharides from purified cell walls of Pseudomonas cepacia
. J. Gen. Microbiol.
112
, 397
–400
.[39]
Strauss
D.C.
Lonon
M.K.
Woods
D.E.
Garner
C.W.
1990
) 3-Deoxy-d-manno-2-octulosonic acid in the lipopolysaccharide of various strains of Pseudomonas cepacia
. J. Med. Microbiol.
33
, 265
–269
.[40]
Anwar
H.
Brown
M.R.W.
Cozens
R.M.
Lambert
P.A.
1983
) Isolation and characterization of the outer and cytoplasmic membranes of Pseudomonas cepacia
. J. Gen. Microbiol.
129
, 499
–507
.[41]
Davidson
D.J.
Dorin
J.R.
McLachlan
G.
Ranaldi
V.
Lamb
D.
Doherty
C.
Govan
J.
Porteous
D.J.
1994
) Lung disease in the cystic fibrosis mouse exposed to bacterial pathogens
. Nature genetics
, in press
.[42]
Elborn
J.S.
Dodd
M.
Maddison
J.
Nixon
L.E.
Nelson
J.
Govan
J.
Webb
A.K.
Shale
D.J.
1994
) Clinical and inflammatory responses in CF patients infected with Pseudomonas aeruginosa (PA) and cepacia (PC)
. 8th North American Cystic Fibrosis Conference
, Florida
, Pediatr. Pulmonol
. Suppl. 10
, pp. 287
.© 1995 Federation of European Microbiological Societies.
Topic:
Issue Section:
Research article
