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G.V. Carbonell, B.A.L. Fonseca, L.T.M. Figueiredo, A.L.C. Darini, R.M. Yanaguita; Culture conditions affect cytotoxin production by Serratia marcescens, FEMS Immunology & Medical Microbiology, Volume 16, Issue 3-4, 1 December 1996, Pages 299–307, https://doi.org/10.1111/j.1574-695X.1996.tb00149.x
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Abstract
Cytotoxins have been implicated in the pathogenesis of bacterial infections. In this study, the influence of different culture conditions was evaluated on cytotoxin production by Serratia marcescens. Parameters such as culture media, incubation temperature, starting pH of culture medium, aeration, anaerobiosis, carbon sources, iron concentration in the culture media, and release of cell-bound toxin by polymyxin B were investigated. The data suggest that this cytotoxin is predominantly extracellular and is not induced by iron limitation. Aerobic culture with shaking resulted in higher cytotoxicity than static aerobic or anaerobic culture. Bacteria grown in glucose, sucrose or galactose were more cytotoxic than those grown in inositol or maltose. The culture conditions that were identified as optimal for cytotoxin production by Serratia marcescens were incubation temperature ranging from 30 to 37°C, in medium adjusted to pH 8.5, with shaking. This work will contribute to further studies on the identification of this cytotoxic activity.
References
Bizette
G.A.
Lindberg
J.S.
Figueroa
J.E.
1995
) Serratia marcescens peritonitis in a patient receiving chronic ambulatory peritoneal dialysis complicated by osteomyelitis
. J. Louisiana State Med. Soc.
147
, 64
–67
.Borenfreund
E.
Puerner
J.A.
1984
) A simple quantitative procedure using monolayer cultures for cytotoxicity assays
. J. Tissue Culture Methods
9
, 7
–9
.Bjorn
M.J.
Iglewski
B.H.
Ives
S.K.
Sadoff
J.C.
Vasil
M.L.
1978
) Effect of iron on yields of exotoxin A in cultures of Pseudomonas aeruginosa PA-103
. Infect. Immun.
19
, 785
–791
.Campbell
J.R.
Diacovo
T.
Baker
C.J.
1992
) Serratia marcescens meningites in neonates
. Pediatr. Infect. Dis. J.
11
, 881
–886
.Carbonell
G.V.
Vidotto
M.C.
1992
) Virulence factors in Serratia marcescens: cell-bound hemolysin and aerobactin. Braz.
. J. Med. Biol. Res.
25
, 1
–8
.Carbonell
G.V.
Levy
C.E.
Vidotto
M.C.
1992
) Serratia marcescens: studies on normal human serum resistance, serogrouping and pathogenicity for mice
. Rev. Microbiol.
23
, 72
–75
.Castañeda-Agulló
M.
1956
) Studies on the biosynthesis of extracellular protease by bacteria. I. Serratia marcescens. Synthetic and gelatin media
. J. Gen. Physiol.
39
, 369
–375
.Donohue-Rolfe
A.
Keusch
G.T.
1983
) Shigella dysenteriae 1 cytotoxin: periplasmic protein releasable by polymyxin B and osmotic shock
. Infect. Immun.
39
, 270
–274
.Finlay
B.B.
Falkow
S.
1989
) Common themes in microbial pathogenicity
. Microbiol. Rev.
53
, 210
–230
.Frank
D.W.
Storey
D.G.
1994
) Regulation of expression of Pseudomonas exotoxin A by iron
. Methods Enzymol.
235
, 502
–510
.Frank
D.W.
Storey
D.G.
Hindahl
M.S.
Iglewski
B.H.
1989
) Differential regulation by iron of rega and toxA transcript accumulation in Pseudomonas aeruginosa
. J. Bacteriol.
170
, 4477
–4483
.Gilligan
P.H.
Robertson
D.C.
1979
) Nutritional requirements for synthesis of heat-labile enterotoxin by enterotoxigenic strains of Escherichia coli
. Infect. Immun.
23
, (1)
, 99
–107
.Haslam
S.C.
Ketley
J.M.
Mitchell
T.J.
Stephen
J.
Burdon
D.W.
Candy
D.C.A.
1986
) Growth of Clostridium difficile and production of toxins A and B in complex and defined media
. J. Med. Microbiol.
21
, 293
–297
.Hernández-Delgadillo
R.
Ruiz-Cruz
J.J.
1994
) Actividad proteolítica libre de Serratia marcescens. II. Cinética de producción y mecanismo de inducción
. Rev. Lat.-Am. Microbiol.
36
, 93
–100
.Jepsen
P.K.
Riise
E.
Biedermann
K.
Kristensen
P.C.
Emborg
C.
1987
) Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease
. Appl. Environ. Microbiol.
53
, 2593
–2596
.Karmali
M.A.
Petric
M.
Lim
C.
Cheung
R.
Arbus
G.S.
1985
) Sensitive method for detecting low numbers of verotoxin producing Escherichia coli in mixed cultures by use of colony sweeps and polymyxin extraction of verotoxin
. J. Clin. Microbiol.
22
, 614
–619
.Konowalchuk
J.
Speirs
J.I.
Stravic
S.
1977
) Vero response to a cytotoxin of Escherichia coli
. Infect. Immun.
18
, 775
–779
.Litwin
C.M.
Calderwood
S.B.
1993
) Role of iron in regulation of virulence genes
. Clin. Microbiol. Rev.
6
, 137
–149
.MacLeod
D.L.
Gyles
C.L.
1989
) Effects of culture conditions on yield of Shiga-like toxin-IIv from Escherichia coli
. Can. J. Microbiol.
35
, 623
–629
.Maeda
H.
Morihara
K.
1995
) Serralysin and related bacterial proteinases
. Methods Enzymol.
248
, 395
–413
.Mahony
D.E.
Gilliatt
E.
Dawson
S.
Stockdale
E.
Lee
S.H.S.
1989
) Vero cell assay for rapid detection of Clostridium perfringes enteroxin
. Appl. Environ. Microbiol.
55
, 2141
–2143
.Matsumoto
K.
Maeda
H.
Takada
K.
Kamata
R.
Okamura
R.
1984
) Purification and characterization of four proteases from a clinical isolate of Serratia marcescens
. J. Bacteriol.
157
, 225
–232
.Maurelli
A.T.
Blackmon
B.
Curtis
R.
1984
) Temperature-dependent expression of virulence genes in Shigella species
. Infect. Immun.
43
, 195
–201
.Meltz
D.J.
Grieco
M.H.
1973
) Characteristics of Serratia marcescens pneumonia
. Arch. Intern. Med.
132
, 359
–364
.McNaughton
M.
Mazinke
N.
Thomas
E.
1995
) Newborn conjunctivitis associated with triclosan 0.5% antiseptic intrinsically contaminated with Serratia marcescens
. Can. J. Infect. Control
10
, 7
–8
.Nakashima
A.K.
McCarthy
M.A.
Martone
W.J.
Anderson
R.L.
1987
) Epidemic arthritis caused by Serratia marcescens and associated with a benzalkonium chloride antiseptic
. J. Clin. Microbiol.
25
, 1014
.Okuda
T.
Endo
N.
Osada
Y.
1981
) Outbreak of nosocomial urinary tract infections caused by Serratia marcescens
. J. Clin. Microbiol.
20
, 691
–695
.Parment
P.A.
Svanborg-Ede'n
C.
Chaknis
M.J.
Sawant
A.D.
Hadberg
L.
Wilson
L.A.
Ahearn
D.G.
1992
) Hemagglutination (fimbriae) and hydrophobicity in adherence of Serratia marcescens to urinary tract epithelium and contact lenses
. Curr. Microbiol.
25
, 113
–118
.Poole
K.
Braun
V.
1988
) Influence of growth temperature and lipopolysaccharide on hemolytic activity of Serratia marcescens
. J. Bacteriol.
170
, 5146
–5152
.Repetto
G.
Sanz
P.
1993
) Neutral red uptake, cellular growth and lysosomal function, in vitro effects of 24 metals
. Alternat. Lab. Animals
21
, 501
–507
.Rowe
G.E.
Welch
R.A.
1994
) Assays of hemolytic toxins
. Methods Enzymol.
235
, 657
–667
.Ruan
Y.
Braun
V.
1990
) Hemolysin as a marker for Serratia
. Arch. Microbiol.
154
, 221
–225
.Rydén
A.C.
Hofsten
B.
1968
) Some properties of extracellular proteinase and the cell-bound peptidase of Serratia
. Acta Chem. Scand.
22
, 2803
–2808
.Sekaninova
G.
Kolarova
M.
Semradova
S.
Taborska
D.
Zajicova
V.
1995
) Nosocomial infections caused by selected gram-negative bacteria at the Anaesthesiology and Intensive Care Unit of the Teaching Hospital in Brno
. Central Eur. J. Public Health
3
, 80
–83
.Serafim
M.B.
Jesus Piton
M.A.
Colli
I.A.G.
Castro
A.F.P.
1994
) Enterotoxin of Aeromonas sp., some culture conditions which affect its production
. Rev. Microbiol.
25
, 220
–224
.Simberkoff
M.S.
Ricupero
I.
JR
R.J.J.
1976
) Host resistance to Serratia marcescens infection: serum bactericidal activity and phagocytosis by normal blood leukocytes
. J. Lab. Clin. Med.
87
, 206
–217
.Stephen
M.
Lalitha
M.K.
1993
) An outbreak of Serratia marcescens infection among obstetric patients. Indian
. J. Med. Res.
97
, 202
–205
.Tai
S.P.
Krafft
A.E.
Nootheti
P.
Holmes
R.K.
1990
) Coordinate regulation of siderophore and diphtheria toxin production by iron in Corynebacterium diphtheriae
. Microbiol. Pathol.
9
, 267
–273
.Volkow-Fernandez
P.
Léon-Rosales
S.P.
Sifuentes-Osornio
J.
Calva-Mercado
J.J.
Ruiz-Palacios
G.M.
Cérbon
M.A.
1993
) Epidemia de bacteremias primarias por una cepa endémica de Serratia marcescens en una unidad de terapia intensiva
. Sal. Públ. México
35
, 440
–447
.Weinstein
D.L.
Holmes
R.K.
O'Brien
A.D.
1988
) Effects of iron and temperature on Shiga-like toxin I production by Escherichia coli
. Infect. Immun.
56
, 106
–111
.Yamakawa
K.
Kamiya
S.
Meng
X.Q.
Karasawa
T.
Nakamura
S.
1994
) Toxin production by Clostridium difficile in a defined medium with limited amino acids
. J. Med. Microbiol.
41
, 319
–323
.Yamamoto
T.
Ariyoshi
A.
Amako
K.
1985
) Fimbriamediated adherence of Serratia marcescens US5 strain to human urinary bladder surface
. Microbiol. Immunol.
29
, 677
–681
.© 1996 Federation of European Microbiological Societies.
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