Collection
Microscopy Revolution
Tâm Mignot, Marcelo Nollmann
Microbiology research has been hugely affected by new advances in imaging techniques. This Thematic Issue explores how these advances are helping to further our understanding of the microbial world around us. The articles in this collection cover a range of topics including RNA imaging in bacteria, bacterial translesion synthesis, stress responses, and super-resolution imaging of pathogens.
Editorial
Reviews
Advances in computational image analysis result in new opportunities for the analysis of spatiotemporal processes in bacterial cells and communities, and perhaps in the near for artificial intelligence-based automated adaptive microscopy in microbiology.
Recent advances in RNA labeling make it possible to study transcript abundance, localization and dynamics in bacteria.
Newly characterized simple principles that describe how bacterial cells grow and divide at the cellular scale need to be related with the underlying complex and interwoven molecular mechanisms.
This review focuses on how recent advances in super-resolution imaging have been applied to studies of bacterial pathogens.
Real-time visualization of tetracycline (Tc) and the Tc-specific TetA efflux pump protein reveals the dynamics of drug accumulation and extrusion in live Escherichia coli cells.
Recent developments in cryo-electron microscopy point to a key role for this technique in future efforts to develop antimicrobial drugs that target the ribosome.
In this review we highlight the application of microscopy-based approaches to unravel the in vivo mechanism and regulation of bacterial translesion synthesis.
This review highlights microscopy approaches that visualise dynamics and heterogeneity of stress responses at single-cell resolution.
